A5102934051- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Lipoproteins and Cardiovascular Health
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Transgenic Plants and Applications
- Advancements in Solid Oxide Fuel Cells
- Diagnosis and treatment of tuberculosis
- Cancer, Lipids, and Metabolism
- Electronic and Structural Properties of Oxides
- Enzyme Structure and Function
- Magnetic and transport properties of perovskites and related materials
- Mitochondrial Function and Pathology
- Lipid metabolism and disorders
- Evasion and Academic Success Factors
- Metabolism and Genetic Disorders
- Education Pedagogy and Practices
- Parasites and Host Interactions
- Science and Science Education
- Plant Genetic and Mutation Studies
- Protein Structure and Dynamics
- Trypanosoma species research and implications
- Nuclear Receptors and Signaling
National Institute of Health Dr. Ricardo Jorge
2013-2022
University of Lisbon
2016-2022
Brazilian Synchrotron Light Laboratory
2001-2008
Universidade de São Paulo
1999-2002
Universidade Brasil
1999
Abstract Mycobacterium tuberculosis drug resistance (DR) challenges effective disease control. Current molecular tests examine limited numbers of mutations, and although whole genome sequencing approaches could fully characterise DR, data complexity has restricted their clinical application. A library (1,325 mutations) predictive DR for 15 anti-tuberculosis drugs was compiled validated 11 them using genomic-phenotypic from 792 strains. rapid online ‘TB-Profiler’ tool developed to report...
Approximately 10% of the Mycobacterium tuberculosis genome is made up two families genes that are poorly characterized due to their high GC content and highly repetitive nature. The PE PPE typified by conserved N-terminal domains incorporate proline-glutamate (PE) proline-proline-glutamate (PPE) signature motifs. They hypothesised be important virulence factors involved with host-pathogen interactions, but genetic variability complexity analysis means they typically disregarded in studies.To...
Nuclear receptors are ligand-inducible transcription factors that share structurally related DNA-binding (DBD) and ligand-binding (LBD) domains. Biochemical structural studies have revealed the modular nature of DBD LBD. Nevertheless, domains function in concert vivo. While high-resolution crystal structures nuclear receptor DBDs LBDs available, there no x-ray proteins containing multiple We report solution human retinoid X DBD-LBD (hRXRalphaDeltaAB) region. obtained ab initio shapes...
Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it an extremely dynamic field. Transgenic plants are commonly used to complete substitute mutants in basic helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception signal transduction. In this review, recent approaches foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, planta...
In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes guianensis we assessed the biological physical parameters affecting plant electroporation protoplasts. Energy input, as combinations of electric field strengths discharged by different capacitors, buffer DNA form were evaluated. Transformation efficiency was assayed in vivo transient reporter gene expression, using GFP-coding mgfp5 driven CaMV 35S constitutive promoter. input strength had critical...
Changes in nominal composition of the perovskite (ABO3) solid solution Ba1-x(Zr,Pr)O3-δ and adjusted firing conditions at very high temperatures were used to induce structural changes involving site redistribution frozen-in point defects, as revealed by Raman photoluminescence spectroscopies. Complementary magnetic measurements allowed quantification reduced content Pr. Weak dependence oxygen stoichiometry with temperature was obtained coulometric titration below 1000 °C, consistent a...
Members of the perovskite solid solution BaZr1–xPrxO3−δ (0.2 ≤ x 0.8) with potential high-temperature electrochemical applications were synthesized via mechanical activation and annealing at 1250 °C. Structural properties examined by Rietveld analysis neutron powder diffraction Raman spectroscopy room temperature, indicating rhombohedral symmetry (space group R3̅c) for members = 0.2 0.4 orthorhombic (Imma) 0.6 0.8. The sequence phase transitions complete from BaZrO3 to BaPrO3 is Pm3̅m → R3̅c...
Hanging-drop vapour-diffusion method was used for the crystallization of extended DNA-binding domain (residues 309-410) E2 protein from Bovine Papillomavirus Type 1. X-ray data collection at 2.0 A resolution performed using synchrotron radiation. The crystal symmetry could be described by space group P3121 and with unit cell parameters a = b 55.3 A, c 203.4 A. structure solved molecular replacement. Keywords: domain, PapillomaVirus type 1