A5072410858- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Parasitic infections in humans and animals
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Parasitic Infections and Diagnostics
- Biosensors and Analytical Detection
- Biochemical and Molecular Research
- Congenital Anomalies and Fetal Surgery
- Pneumocystis jirovecii pneumonia detection and treatment
- Digital Imaging for Blood Diseases
- Infectious Diseases and Tuberculosis
- Pneumonia and Respiratory Infections
- Cancer therapeutics and mechanisms
- Advanced biosensing and bioanalysis techniques
- Diagnosis and treatment of tuberculosis
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- vaccines and immunoinformatics approaches
- Synthesis and biological activity
- Autism Spectrum Disorder Research
- Viral gastroenteritis research and epidemiology
- Probiotics and Fermented Foods
- Metal Extraction and Bioleaching
- Amoebic Infections and Treatments
Universidad Peruana Cayetano Heredia
2015-2024
Farvet (Peru)
2022
St George's, University of London
2021
Centro de Investigación y Desarrollo
2019
Groote Schuur Hospital
2018
University of Cape Town
2018
Johns Hopkins University
1998-2009
National University of San Marcos
2009
Prisma
1995-2008
University of Concepción
2008
Objective: Lactobacillus GG (L-GG), an acid- and bile-resistant strain that colonizes the intestinal mucosa, has been used to manage diarrhea in children. Our objective was evaluate prophylactic use of L-GG prevent children at high risk from a developing country randomized, placebo-controlled trial. Study design: Two hundred four undernourished 6 24 months old indigent peri-urban Peruvian town received either or placebo flavored gelatin once daily, days week, for 15 months. Episodes were...
ABSTRACT Inexpensive, rapid, and reliable methods of detecting infection by drug susceptibility Mycobacterium tuberculosis (MTB) are crucial to the control tuberculosis. The novel microscopic observation broth-drug assay (MODS) detects early growth MTB in liquid medium, allowing more timely diagnosis testing. Sputum samples from hospitalized patients Peru were analyzed using stains, culture, PCR. Sensitivity MODS (92%) compared favorably with most sensitive other culture (93%). positive for...
Background Institutional tuberculosis (TB) transmission is an important public health problem highlighted by the HIV/AIDS pandemic and emergence of multidrug- extensively drug-resistant TB. Effective TB infection control measures are urgently needed. We evaluated efficacy upper-room ultraviolet (UV) lights negative air ionization for preventing airborne using a guinea pig air-sampling model to measure infectiousness ward air. Methods Findings For 535 consecutive days, exhaust from HIV-TB in...
The current understanding of airborne tuberculosis (TB) transmission is based on classic 1950s studies in which guinea pigs were exposed to air from a ward. Recently we recreated this model Lima, Perú, and paper report the use molecular fingerprinting investigate patient infectiousness era HIV infection multidrug-resistant (MDR) TB.
Approximately 10% of the Mycobacterium tuberculosis genome is made up two families genes that are poorly characterized due to their high GC content and highly repetitive nature. The PE PPE typified by conserved N-terminal domains incorporate proline-glutamate (PE) proline-proline-glutamate (PPE) signature motifs. They hypothesised be important virulence factors involved with host-pathogen interactions, but genetic variability complexity analysis means they typically disregarded in studies.To...
Background.Nosocomial transmission of tuberculosis remains an important public health problem. We created in vivo air sampling model to study airborne from patients coinfected with human immunodeficiency virus (HIV) and evaluate environmental control measures.
Beijing family strains of Mycobacterium tuberculosis have attracted worldwide attention because their wide geographical distribution and global emergence. Peru, which has a historical relationship with East Asia, is considered to be hotspot for in South America. We aimed unveil the genetic diversity transmission characteristics Peru. A total 200 were identified from 2140 M. isolates obtained Lima, between December 2008 January 2010. Of them, 198 classified into sublineages, on basis 10 sets...
Congregate settings may serve as institutional amplifiers of tuberculosis (TB) and multidrug-resistant (MDR-TB). We analyze spatial, epidemiological, pathogen genetic data prospectively collected from neighborhoods surrounding a prison in Lima, Peru, where inmates experience high risk MDR-TB, to investigate the spillover into community.Using hierarchical Bayesian statistical modeling, we address three questions regarding MDR-TB risk: (i) Does excess observed among prisoners also extend...
Tuberculosis is an infectious disease that causes ill health and death in millions of people each year worldwide. Timely diagnosis treatment key to full patient recovery. The Microscopic Observed Drug Susceptibility (MODS) a test diagnose TB infection drug susceptibility directly from sputum sample 7–10 days with low cost high sensitivity specificity, based on the visual recognition specific growth cording patterns M. broth culture. Despite its advantages, MODS still limited remote, resource...
Abstract SARS-CoV-2 main protease is a common target for inhibition assays due to its high conservation among coronaviruses. Since flavonoids show antiviral activity, several in silico works have proposed them as potential inhibitors. Nonetheless, there reason doubt certain results given the lack of consideration flavonoid promiscuity or plasticity, usage short library sizes, absence control molecules and/or limitation methodology single site. Here, we report virtual screening study where...
Objective. We evaluated the clinical and epidemiologic characteristics of Peruvian children presenting with pulmonary tuberculosis (PTB) to determine whether features predictive confirmed PTB could be identified. Study Design. This was a cross-sectional study 135 (mean age: 6.8 years) Hospital del Niño in Lima, Peru, presumptive diagnosis PTB. Clinical, epidemiologic, laboratory findings were compared between 3 groups pediatric patients PTB: those positive Mycobacterium (MTB) cultures,...
A novel heminested IS6110 polymerase chain reaction (PCR) assay was evaluated as a tool for diagnosing tuberculosis in 222 children. In an analysis of 392 specimens (gastric aspirates, nasopharyngeal and sputum samples), results PCR were compared with those 3 culture methods, acid-fast bacillus (AFB) staining, clinical assessment by the Stegen-Toledo score. The sensitivity (67%) comparable to that 3-culture method (71%) significantly higher than Löwenstein-Jensen (54%) or AFB stain (42%)...
Tuberculosis (TB) pleural disease is complicated by extensive tissue destruction. Matrix metalloproteinase (MMP)-1 and -9 are implicated in immunopathology of pulmonary central nervous system TB. There few data on MMP activity TB pleurisy. The present study investigated MMP-1, -2 their specific inhibitors (tissue inhibitor (TIMP)-1 -2) tuberculous effusions, correlated these with clinical histopathological features. Clinical data, routine blood tests, fluid/biopsy material were obtained from...
Increasing awareness of the environmental risks posed by heavy metal accumulation in environment—due to their toxicity and persistence biological systems—has driven development more efficient accessible detection methods. Conventional techniques, despite accuracy, are often expensive, time-consuming, reliant on non-portable specialized equipment. This study presents a novel, low-cost electrochemical sensor using fluorine-doped tin oxide (FTO) electrode modified with Nafion-stabilized silver...
Background There is limited available data on the strain diversity of M tuberculosis in Peru, though there may be interesting lessons to learn from a setting where multidrug resistant TB has emerged as major problem despite an apparently well-functioning DOTS control programme. Methods Spoligotyping was undertaken 794 strains collected between 1999 and 2005 553 community-based patients 241 hospital-based HIV co-infected with pulmonary Lima, Peru. Phylogenetic epidemiologic analyses permitted...
Campylobacter jejuni is a major cause of diarrhea among children in developing countries. Since free-ranging chickens are source infections, we hypothesized that corralling these would result decreased rates infections and Campylobacter-related diarrhea. We tested this hypothesis Peruvian families periruban shantytown with randomized by household using (corralling) intervention versus control study design. Samples from participants were cultured for at the start surveillance, samples less...
Pediatric pulmonary tuberculosis diagnosis is difficult because young children are unable to expectorate sputum samples. Testing stool for DNA from swallowed may diagnose tuberculosis. Hospitalized with suspected had stool, nasopharyngeal, and gastric aspirates cultured that confirmed in 16/236 patients. Twenty-eight stored stools these 16 were used evaluate polymerase chain reaction (PCR) compared 28 samples 23 healthy control children. Two extraction techniques used: fast-DNA mechanical...
Pulmonary tuberculosis diagnosis is difficult when patients cannot produce sputum. Most sputum swallowed, and DNA can survive intestinal transit. We therefore evaluated molecular testing of stool specimens for detecting originating from the lungs. Paired samples (n=159) were collected 89 with pulmonary tuberculosis. Control (n=47) without symptoms. Two techniques extraction compared, diagnostic accuracy PCR in was compared by PCR, microscopy, culture. A heminested IS6110-PCR used detection,...
Tuberculosis (TB) is a major global health problem and drug resistance compromises the efforts to control this disease. Pyrazinamide (PZA) an important used in both first second line treatment regimes. However, its complete mechanism of action remains unclear.
Background The comparison of Mycobacterium tuberculosis bacterial genotypes with phenotypic, demographic, geospatial and clinical data improves our understanding how strain lineage influences the development drug-resistance spread tuberculosis. Methods To investigate association genotype drug-resistance. Drug susceptibility testing together genotyping using both 15-loci MIRU-typing spoligotyping, was performed on 2,139 culture positive isolates, each from a different patient in Lima, Peru....