Manuel Criollo-Orozco
A5080645530- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Computational Drug Discovery Methods
- Vector-Borne Animal Diseases
- Animal Genetics and Reproduction
- Herpesvirus Infections and Treatments
- Transgenic Plants and Applications
- Animal Disease Management and Epidemiology
- Plant biochemistry and biosynthesis
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Pharmacological Effects of Natural Compounds
- COVID-19 Clinical Research Studies
Farvet (Peru)
2018-2024
University of Maryland, Baltimore County
2021
Abstract SARS-CoV-2 main protease is a common target for inhibition assays due to its high conservation among coronaviruses. Since flavonoids show antiviral activity, several in silico works have proposed them as potential inhibitors. Nonetheless, there reason doubt certain results given the lack of consideration flavonoid promiscuity or plasticity, usage short library sizes, absence control molecules and/or limitation methodology single site. Here, we report virtual screening study where...
In this study, we developed a new recombinant virus rHVT-F using Turkey herpesvirus (HVT) vector, expressing the fusion (F) protein of genotype XII Newcastle disease (NDV) circulating in Peru. We evaluated viral shedding and efficacy against NDV challenge specific pathogen-free (SPF) chickens. The F expression cassette was inserted unique long (UL) UL45-UL46 intergenic locus HVT genome by utilizing clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 gene-editing technology...
COVID-19 pandemic has accelerated the development of vaccines against its etiologic agent, SARS-CoV-2. However, emergence new variants virus lead to generation alternatives improve current sub-unit in development. In present report, immunogenicity Spike RBD SARS-CoV-2 formulated with an oil-in-water emulsion and a water-in-oil squalene was evaluated mice hamsters. The protein expressed insect cells purified by chromatography until >95% purity. shown have appropriate folding as determined...
Background and Aim: Gumboro disease is an economically crucial veterinary condition in chickens. It caused by the infectious bursal virus (IBDV). This consists of two serotype groups, which I strain pathogenic to For many years, development molecular techniques for either diagnostic purposes or surveillance appearance new strains has mainly focused on targeting VP2 genomic region. However, due constant necessity discrimination between already prevalent vaccine this virus, it becomes...
Abstract The coronavirus disease-19 (COVID-19) pandemic has already claimed millions of lives and remains one the major catastrophes in recorded history. While mitigation control strategies provide short term solutions, vaccines play critical roles long disease. Recent emergence potentially vaccine-resistant novel variants necessitated testing deployment technologies that are safe, effective, stable, easy to administer, inexpensive produce. Here we developed three recombinant Newcastle...
ABSTRACT The COVID-19 pandemic has claimed the lives of millions people worldwide and threatens to become an endemic problem, therefore need for as many types vaccines possible is high importance. Because doses required, it desirable that are not only safe effective, but also easy administer, store, inexpensive produce. Newcastle Disease Virus (NDV) responsible a respiratory disease in chickens. It no pathogenic homologue humans. NDV recognized oncolytic virus, its use humans oncological...
Abstract COVID-19 pandemic has accelerated the development of vaccines against its etiologic agent, SARS-CoV-2. However, emergence new variants virus requires immunization strategies in addition to current approved for human administration. In present report, immunological and safety evaluation mice hamsters a subunit vaccine based on RBD sub-domain with two adjuvants oil origin is described. The protein was expressed insect cells purified by chromatography until >95% purity. shown have...
El estudio tuvo por objetivo desarrollar una plataforma molecular para la cuantificación del virus de enfermedad Newcastle (NDV) a partir un sistema cultivo en huevos embrionados SPF. Primero se evaluaron cuatro pares cebadores que amplifican diferentes regiones genoma viral NDV codifican proteína nucleocápside (NP), matriz (M), fusión (F) y ARN polimerasa dependiente (L), con finalidad seleccionar el más conservado cual desarrolló basada transcripción reversa - reacción cadena convencional...
<title>Abstract</title> The COVID-19 pandemic has claimed the lives of millions people. Vaccination is a critical tool for control transmission; however, recent emergence potentially vaccine-resistant variants renders it important to have range vaccines types. It desirable that are safe, effective, easy administer and store, inexpensive produce. Newcastle disease virus (NDV), responsible respiratory in chickens, no pathogenic homologue humans. We developed two types NDV-vectored candidate...