A5079873268- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- interferon and immune responses
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- MicroRNA in disease regulation
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- RNA regulation and disease
- T-cell and Retrovirus Studies
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Vector-borne infectious diseases
- RNA and protein synthesis mechanisms
- Poxvirus research and outbreaks
- RNA Interference and Gene Delivery
- Microbial infections and disease research
- Circular RNAs in diseases
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
The Pirbright Institute
2016-2025
University of Oxford
1992-2024
Jenner Institute
2009-2024
Henan Academy of Agricultural Sciences
2021
Global Hospitals
2012-2020
Universidad de Antioquia
2019
Universidad Nacional de Colombia
2019
Biotechnology and Biological Sciences Research Council
2014-2016
Harbin Veterinary Research Institute
2016
Chinese Academy of Agricultural Sciences
2016
Could some vaccines drive the evolution of more virulent pathogens? Conventional wisdom is that natural selection will remove highly lethal pathogens if host death greatly reduces transmission. Vaccines keep hosts alive but still allow transmission could thus very strains to circulate in a population. Here we show experimentally immunization chickens against Marek's disease virus enhances fitness strains, making it possible for hyperpathogenic transmit. Immunity elicited by direct...
Notwithstanding the well-characterised roles of a number oncogenes in neoplastic transformation, microRNAs (miRNAs) are increasingly implicated several human cancers. Discovery miRNAs oncogenic herpesviruses such as KSHV has further highlighted potential virus-encoded to contribute their capabilities. Nevertheless, despite identification possible cancer-related genes targets, direct vivo role diseases those induced by is difficult demonstrate absence suitable models. However, excellent...
ABSTRACT Kaposi's sarcoma-associated herpesvirus-encoded microRNA (miRNA) MiR-K12-11 was recently shown to be a functional ortholog of miR-155, miRNA that plays major role in lymphoid malignancies and the modulation immune responses. Here we show miR-M4, encoded by highly oncogenic Marek's disease virus chickens, shares common targets with miR-155 thus is also first one identified an alphaherpesvirus. The observation two distinct herpesviruses associated types lymphomas different species...
Highly polymorphic major histocompatibility complex (MHC) molecules are at the heart of adaptive immune responses, playing crucial roles in many kinds disease and vaccination. We report that breadth peptide presentation level cell surface expression class I inversely correlated both chickens humans. This relationship correlates with protective responses against infectious pathogens including Marek's virus leading to lethal tumours human immunodeficiency infection progressing AIDS propose...
Research over the last few years has demonstrated increasing role of microRNAs (miRNAs) as major regulators gene expression in diverse cellular processes and diseases. Several viruses, particularly herpesviruses, also use miRNA pathway regulation by encoding their own miRNAs. Marek's disease (MD) is a widespread lymphomatous neoplastic poultry caused highly contagious virus type 1 (MDV-1). Recent studies using virus-infected chicken embryo fibroblasts have identified at least eight miRNAs...
Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones highly RB-1B strain by inserting mini-F vector sequences into U(S)2 locus. MDV reconstituted from two BAC induced rapid-onset similar to those wild-type virus. Virus another clone showed a 7.7-kbp deletion internal and terminal unique long repeat regions was nononcogenic, suggesting deleted region may be associated...
Marek’s disease virus (MDV) is an oncogenic herpesvirus that induces fatal T cell lymphomas in chickens. With more than 20 billion doses of vaccine used annually, vaccination constitutes the cornerstone control. Despite success vaccination, evolution virulence among MDV strains continues to threaten effectiveness current vaccines. MDV-encoded protein MEQ (MDV EcoRI Q) probably acts as a transcription factor and considered be major oncoprotein. sequence shows Pro-Leu-Asp-Leu-Ser (PLDLS) motif...
Telomerase is a ribonucleoprotein complex consisting of two essential core components: reverse transcriptase and an RNA subunit (telomerase [TR]). Dysregulation telomerase has been associated with cell immortalization oncogenesis. Marek's disease herpesvirus (MDV) induces malignant T lymphoma in chickens harbors its genome identical copies viral TR (vTR) 88% sequence identity to chicken TR. MDV mutants lacking both vTR were significantly impaired their ability induce lymphomas, although...
ABSTRACT Marek's disease virus (MDV) causes a general malaise in chickens that is mostly characterized by the development of lymphoblastoid tumors multiple organs. The use bacterial artificial chromosomes (BACs) for cloning and manipulation MDV genome has facilitated characterization specific genes genomic regions. most BACs, including pRB-1B-5, derived from very virulent strain, involved replacement U S 2 gene with mini-F vector sequences. However, when reconstituted viruses based on pRB-1B...
ABSTRACT MicroRNAs (miRNAs) are increasingly being recognized as major regulators of gene expression in many organisms, including viruses. Among viruses, members the family Herpesviridae account for majority currently known virus-encoded miRNAs. The highly oncogenic Marek's disease virus type 1 (MDV-1), an avian herpesvirus, has recently been shown to encode eight miRNAs clustered MEQ and LAT regions viral genome. genus Mardivirus , which MDV-1 belongs, also includes nononcogenic but...
ABSTRACT Marek's disease virus (MDV) is a highly contagious oncogenic alphaherpesvirus that causes both cancer model and continuing threat to the world's poultry industry. This comprehensive gene expression study analyzes host response infection in resistant susceptible lines of chickens inherent differences between two following host. A novel pathogenicity mechanism, involving downregulation genes containing HIC1 transcription factor binding sites as early 4 days postinfection, was...
Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely a vector platform generation recombinant vaccines number avian diseases such infectious bursal (IBD), Newcastle (ND) and influenza (AI) using conventional recombination methods recombineering tools on cloned viral genomes. In the present study, we describe application CRISPR/Cas9-based...