A5005373107- Gastrointestinal Bleeding Diagnosis and Treatment
- Gastrointestinal disorders and treatments
- RNA and protein synthesis mechanisms
- Cervical Cancer and HPV Research
- Esophageal and GI Pathology
- Protein Structure and Dynamics
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Screening and Detection
- RNA Research and Splicing
- Virus-based gene therapy research
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Respiratory viral infections research
- Gastrointestinal Tumor Research and Treatment
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- Hepatitis B Virus Studies
- Gastroesophageal reflux and treatments
- Viral gastroenteritis research and epidemiology
- Genetic factors in colorectal cancer
- Ubiquitin and proteasome pathways
- interferon and immune responses
Fundación Instituto Leloir
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2025
Brazilian Center for Research in Energy and Materials
2023
Brazilian Biosciences National Laboratory
2023
Universidade Federal do Rio de Janeiro
1996-2016
W. M. Keck Foundation
2014
Yale University
2014
Hôpitaux Universitaires de Strasbourg
2013
Délégation Centre-Est
2008-2012
Azienda Ospedaliero Universitaria San Giovanni Battista
2012
This study was designed to prospectively compare the diagnostic yield of M2A endoscopic capsule with that video push-enteroscopy in exploring small intestine patients obscure digestive bleeding.Patients either occult or overt bleeding and a negative work-up underwent double intestinal investigation, wireless capsule, performed blindly by separate examiners. The for each technique defined as frequency detection clinically relevant lesions, i. e. those having potential bleeding.60 (27 men, 33...
PillCam colon capsule endoscopy (CCE) is an innovative noninvasive, and painless ingestible technique that allows exploration of the without need for sedation gas insufflation. Although it already available in European other countries, clinical indications CCE as well reporting work-up detected findings have not yet been standardized. The aim this evidence-based consensus-based guideline, commissioned by Society Gastrointestinal Endoscopy (ESGE) to furnish healthcare providers with a...
Background and Study Aims: In patients with obscure digestive bleeding, the bleeding source is frequently located in small bowel. Capsule endoscopy (CE) an effective method of investigating whole bowel such patients. present study, a diagnostic approach was tested which CE included as initial examination when esophagogastroduodenoscopy (EGD) colonoscopy findings were normal. Patients Methods: admitted between October 2000 February 2002 for underwent intestinal investigation, further...
Protein folding in the cell involves action of different molecular chaperones and folding-facilitating enzymes. In endoplasmic reticulum (ER), status glycoproteins is stringently controlled by a glucosyltranferase enzyme (GT) that creates monoglucosylated structures recognized ER resident lectins (calnexin/calreticulin, CNX/CRT). GT serves as sensor because it only glucosylates misfolded or partly folded glycoproteins. Nevertheless, mechanism behind this recognition process remains largely...
The main complication of video capsule endoscopy (VCE) is the blocking by a gastrointestinal stenosis. "patency capsule" self-dissolving that same size as capsule. It contains radiofrequency identification (RFID) tag allows it to be detected scanning device placed on abdominal wall. When its passage blocked stenosis, patency dissolves in 40-80 hours after ingestion. aim this study was evaluate usefulness system patients with suspected intestinal stenosis but also requiring VCE.22 (16 men; 46...
The UDP-Glc:glycoprotein glucosyltransferase (GT), a key player in the endoplasmic reticulum (ER) quality control of glycoprotein folding, only glucosylates glycoproteins displaying non-native conformations. To determine whether GT recognizes folding intermediates or irreparably misfolded species with nearly native structures, we generated and tested as substrates neoglycoprotein fragments derived from chymotrypsin inhibitor 2 (GCI2) bearing 53 to 64 (full-length) amino acids. Fragment...
Correction to: Literature review and recommendations for clinical application of small-bowel capsule endoscopy, based on a panel discussion by international expertsEndoscopy 2007; 39(10): 895-909DOI: 10.1055/s-2007-966930
The long-term outcome for patients with obscure bleeding after capsule endoscopy (CE) is still unclear. In this study, the clinical was used as gold standard to determine sensitivity and specificity of CE push enteroscopy (PE) in diagnosis small-bowel lesions bleeding.Fifty-eight from a previous prospective study (comparing PE CE) were included; contacted 1 year. final diagnosis, status, new gastrointestinal examinations, treatments performed recorded. On basis these data, each case...
A new clinicopathological disorder associated with a paramyxovirus infection in pigs is described. Central nervous system manifestations and corneal opacity are the main features piglets two to 21 days old. Older seem be more resistant only commonly observed. In pregnant sows virus appears responsible for reproductive disturbances such as stillbirth, mummification return oestrus. The changes mainly microscopic characterised by non-suppurative encephalomyelitis, interstitial pneumonia...
A 26-year-old woman was admitted for the investigation of abdominal symptoms related to ileal Crohn's disease. The patient had been diagnosed 3 years previously with systemic sclerosis, and experiencing digestive complaints 6 months. first computed tomography (CT) scan showed intestinal mucosal alterations, associated a sclerolipomatosis suspicion stenosis. An ileocolonoscopy then performed ulcers in terminal ileum nonspecific inflammatory changes found on biopsies, both suggesting diagnosis...
Abstract The equilibrium unfolding reaction of the C‐terminal 80‐amino‐acid dimeric DNA‐binding domain human papillomavirus (HPV) strain 16 E2 protein has been investigated using fluorescence, far‐UV CD, and sedimentation. stability HPV‐16 is concentration‐dependent, well described as a two‐state transition from folded dimer to unfolded monomer. conformational protein, Δ G H2O , was found be 9.8 kcal/mol at pH 5.6, with corresponding unfolding/dissociation constant, K u being 6.5 → 10 −8 M....
The tumor suppressor p53 modulates the transcription of a variety genes constituting protective barrier against anomalous cellular proliferation. High frequency hot-spot mutations result in loss- of-function by formation amyloid-like aggregates that correlate with cancerous progression. We show full-length undergoes spontaneous homotypic condensation at sub-micromolar concentrations and absence crowders, to yield dynamic coacervates are stoichiometrically dissolved DNA. These fuse evolve...
We have prepared a family of peptide fragments the 64-residue chymotrypsin inhibitor 2, corresponding to its progressive elongation from N terminus. The growing polypeptide chain has little tendency form stable structure until it is largely synthesized, and what structures are formed nonnative lack, in particular, native secondary structural elements alpha-helix beta-sheet. These then develop as sufficient tertiary interactions made nearly full-length chain. growth small module highly...
The HPV16 E7 oncoprotein is an extended dimer, with a stable and cooperative fold, but that displays properties of "natively unfolded" proteins. Two regions conserved sequence are found in proteins, where the N-terminus (1-40) includes retinoblastoma tumor suppressor binding casein kinase II phosphorylation sites. A fragment containing highly acidic N-terminal half shows apparently disordered conformation by far-UV-circular dichroism (CD) at neutral pH, its hydrodynamic radius much larger...
The endoscopes that were developed for double-balloon enteroscopy have been successfully used in cases of failed colonoscopy. This study was a pilot series which new colonoscope tested utilized this principle.A total 29 patients (5 men, 24 women; mean age 54 years) whom conventional colonoscopy had included study. Both the and procedures performed under general anesthesia, usual practice France. A prototype instrument (working length 152 cm, diameter 9.4 mm) designed to incorporate...
DNA tumor viruses ensure genome amplification by hijacking the cellular replication machinery and forcing infected cells to enter S phase. The retinoblastoma (Rb) protein controls G1/S checkpoint, is targeted several viral oncoproteins, among these E7 from human papillomaviruses (HPVs). A quantitative investigation of interaction mechanism between HPV16 RbAB domain in solution revealed that 90% binding energy determined LxCxE motif, with an additional determinant (1.0 kcal·mol −1 ) located...