A5067182517- Mesenchymal stem cell research
- Liver physiology and pathology
- Parasites and Host Interactions
- Liver Disease Diagnosis and Treatment
- Marine Sponges and Natural Products
- Research on Leishmaniasis Studies
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Glycosylation and Glycoproteins Research
- Periodontal Regeneration and Treatments
- Macrophage Migration Inhibitory Factor
- Marine Biology and Ecology Research
- Proteoglycans and glycosaminoglycans research
- Bone Tissue Engineering Materials
- Transplantation: Methods and Outcomes
- Galectins and Cancer Biology
- Antioxidant Activity and Oxidative Stress
- Marine Biology and Environmental Chemistry
- Organ Transplantation Techniques and Outcomes
- Trypanosoma species research and implications
- Alkaline Phosphatase Research Studies
- Retinoids in leukemia and cellular processes
- Pancreatic function and diabetes
- Wound Healing and Treatments
- Adenosine and Purinergic Signaling
Faculdade de Medicina de Petrópolis
2014-2024
Bank of Brazil
2024
Universidade Federal do Rio de Janeiro
2011-2023
University of Verona
2016-2020
Zero to Three
2020
Center of Regenerative Medicine in Barcelona
2017-2019
Faculty (United Kingdom)
2019
Institute of Biomedical Science
2016-2017
Nanomed (Brazil)
2016-2017
Universidade Estadual Paulista (Unesp)
2017
Background— This study evaluated the hypothesis that transendocardial injections of autologous mononuclear bone marrow cells in patients with end-stage ischemic heart disease could safely promote neovascularization and improve perfusion myocardial contractility. Methods Results— Twenty-one were enrolled this prospective, nonrandomized, open-label (first 14 patients, treatment; last 7 control). Baseline evaluations included complete clinical laboratory evaluations, exercise stress (ramp...
Platelet-rich plasma (PRP) is nowadays widely applied in different clinical scenarios, such as orthopedics, ophthalmology and healing therapies, a growth factor pool for improving tissue regeneration. Studies into its efficiency are not conclusive one of the main reasons this that PRP preparations used, eliciting responses cannot be compared. Platelet quantification content definition must defined order to understand molecular mechanisms behind regenerative strength. Standardization thus...
Background— We recently reported the safety and feasibility of autologous bone marrow mononuclear cell (ABMMNC) injection into areas ischemic myocardium in patients with end-stage cardiomyopathy. The present study evaluated efficacy this therapy at 6- 12-month follow-up. Methods Results— Twenty follow-up (11 treated subjects; 9 controls) were enrolled prospective, nonrandomized, open-label study. Complete clinical laboratory evaluations as well exercise stress (ramp treadmill), 2-dimensional...
Different mesenchymal stromal cells (MSC) have been successfully isolated and expanded in vitro nowadays they are tested clinical trials for a wide variety of diseases. Whether all MSC express the same cell surface markers or similar secretion profile is still controversial, making it difficult to decide which may be better particular application.We human from bone marrow (BM), adipose tissue (AT) Wharton's jelly (WJ) cultured them fetal bovine serum supplemented media. We evaluated...
The regenerative property of fat grafting has been described. However, it is not clear whether the clinical results are attributable to stem cells or linked other components adipose tissue. This work aimed at analysis histologic and ultrastructural changes aged facial skin after injection graft in addition its stromal vascular fraction, obtained by centrifugation, compare with those expanded adipose-derived mesenchymal cells.This study was performed six consecutive patients who were...
Lycopene, a major carotenoid component of tomato, has potential anticancer activity in many types cancer. Epidemiological and clinical trials rarely provide evidence for mechanisms the compound's action, studies on its effect cancer different cell origins are now being done. The aim present study was to determine lycopene cycle viability eight human lines. Human lines were treated with (1–5 μM) 48 96 h. Cell monitored using method MTT. analyzed by flow cytometry, apoptotic cells identified...
In a previous study, the authors demonstrated that treatment with expanded adipose-derived stem cells or stromal vascular fraction (SVF)-enriched fat modify pattern of dermis in human beings, representing skin rejuvenation effect. Considering require cell factor, wanted to assess similar results by replacing them platelet-rich plasma (PRP), which is easier obtain and for an empirical regenerative effect has been already described. To determine if PRP injection could replace cutaneous cells....
Abstract Dentistry and orthopedics are undergoing a revolution in order to provide more reliable, comfortable long-lasting implants patients. Titanium (Ti) titanium alloys have been used dental total hip arthroplasty due their excellent biocompatibility. However, Ti-based human body suffer surface degradation (corrosion wear) resulting the release of metallic ions solid wear debris (mainly dioxide) leading peri-implant inflammatory reactions. Unfortunately, our current understanding...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 178:205-219 (1999) - doi:10.3354/meps178205 Establishment of a primary cell culture from sponge: primmorphs Suberites domuncula Werner E. G. Müller1,*, Matthias Wiens1, Renato Batel2, Renate Steffen1, Heinz C. Schröder1, Radovan Borojevic3, Marcio Reis Custodio1,3 1Institut für Physiologische Chemie,...
Centrifugation is one of the preferred methods fat processing. Although it has been promoted for nearly three decades to separate adipose tissue components before grafting, there remain many controversies regarding results obtained with centrifuged tissue.The authors demonstrate effects centrifugation on cellular aspirated fat.Fat harvested from lower abdomen 10 female patients undergoing liposuction was divided in two equal parts, then processed by decantation or and sent laboratory. Each...
Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, subendosteal niche is its cellular composition role each cell population in fate have not been established. In vivo models involve subtle species-specific differences, while bidimensional cultures do reflect 3D tissue organization. The aim this study was to investigate vitro human...
F. R. Pereira Lopes, B. C. G. Lisboa, Frattini, M. Almeida, A. Tomaz, P. K. Matsumoto, Langone, S. Lora, Melo, Borojevic, W. Han and Martinez (2011) Neuropathology Applied Neurobiology37, 600–612 Enhancement of sciatic nerve regeneration after vascular endothelial growth factor (VEGF) gene therapy Aims: Recent studies have emphasized the beneficial effects on neurone survival Schwann cell proliferation. VEGF is a potent angiogenic factor, angiogenesis has long been recognized as an important...
Background Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during vitro expansion. The effect supplementation changes on growth and protein production not fully characterized. Methods Human mesenchymal stromal from bone marrow, adipose tissue Wharton's Jelly were isolated cultured PRP-supplemented media. Proliferation, differentiation, expression surface markers, mRNA key genes secretion...
Abstract Introduction Stem cell therapy with bone marrow-derived mononuclear cells (BMMCs) is an option for improving joint function in osteonecrosis of the femoral head (ONFH). Bone mesenchymal stromal (MSC) numbers and their osteogenic differentiation are decreased patients ONFH. However, whether this decrease also extends to early stages ONFH sickle disease (SCD) still unclear. Methods We conducted a phase I/II, non-controlled study determine efficacy safety BMMC implantation using...
Background/Objectives The pathological condition of obesity is accompanied by a dysfunctional adipose tissue. We postulate that subcutaneous, preperitoneal and visceral obese abdominal white tissue depots could have stromal vascular fractions (SVF) with distinct composition stem cells (ASC) would differentially account for the pathogenesis obesity. Methods In order to evaluate distribution SVF subpopulations, samples tissues from morbidly women (n = 12, BMI: 46.2±5.1 kg/m2) were collected...
Abstract Introduction Subcutaneous adipose tissue is an interesting source of autologous stem cells with a fundamental role in the pathophysiology obesity, metabolic syndromes and insulin resistance. We hypothesize that obesity could alter stromal-vascular fraction (SVF) cell (ASCs) functions, which compromise its regenerative behavior. Furthermore, we aimed to evaluate whether ASCs derived from post bariatric surgery ex-obese women maintain their functions similar fashion as do those...
Amphoriscus is a widespread genus with 17 species. A new species was found in SE Brazil and it represents the second of this Brazilian waters. pedunculatus sp. nov. has special structure, peduncle, to attach substrate. Special attachment structures are not very common class Calcarea but third peduncle. Besides another structure some root-tuft, an composed diactines anchor-like triactines or tetractines. The evolution these known they have also been out genus, suggesting that appeared several...