A5021718033- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Synthesis and Biological Evaluation
- Phytochemical compounds biological activities
- Parasites and Host Interactions
- Extracellular vesicles in disease
- Parasitic Infections and Diagnostics
- Insect Pest Control Strategies
- Toxoplasma gondii Research Studies
- Papaya Research and Applications
- Antifungal resistance and susceptibility
- Venomous Animal Envenomation and Studies
- Nanoparticles: synthesis and applications
- Cancer therapeutics and mechanisms
- X-ray Diffraction in Crystallography
- Enzyme function and inhibition
- Peptidase Inhibition and Analysis
- Crystallization and Solubility Studies
- Synthesis and biological activity
- COVID-19 Impact on Reproduction
- Metal complexes synthesis and properties
- Autophagy in Disease and Therapy
- Chemical synthesis and pharmacological studies
- Coccidia and coccidiosis research
- Synthesis and Characterization of Heterocyclic Compounds
Universidade Federal do Rio de Janeiro
2014-2024
University of Rio Grande and Rio Grande Community College
2023
National Institute of Science and Technology for Structural Biology and Bioimaging
2011-2022
Northwestern University
2020
Instituto de Biofísica Carlos Chagas Filho
2008-2018
Fundação Carlos Chagas
2018
Instituto Nacional de Metrologia, Qualidade e Tecnologia
2012-2015
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2008-2015
University of Georgia
2007-2011
Instituto de Parasitología y Biomedicina "López - Neyra"
2003
Sterols are constituents of the cellular membranes that essential for their normal structure and function. In mammalian cells, cholesterol is main sterol found in various membranes. However, other sterols predominate eukaryotic microorganisms such as fungi protozoa. It now well established an important metabolic pathway members Trypanosomatidae family one produces a special class sterols, including ergosterol, 24-methyl which required parasitic growth viability, but absent from host cells....
Toxoplasma gondii belongs to the phylum Apicomplexa and is an important cause of congenital disease infection in immunocompromised patients. Like most apicomplexans, T. possesses several plant-like features, such as chloroplast-like organelle, apicoplast. We describe characterize a novel organelle tachyzoites, which visible by light microscopy broad similarity plant vacuole. Electron tomography shows interaction this vacuole with other organelles. The presence vacuolar proton pyrophosphatase...
Leishmaniasis, caused by protozoan parasites of the Leishmania genus, is one most prevalent neglected tropical diseases. It endemic in 98 countries, causing considerable morbidity and mortality. Pentavalent antimonials are first line treatment for leishmaniasis except India. In resistant cases, miltefosine, amphotericin B pentamidine used. These treatments unsatisfactory due to toxicity, limited efficacy, high cost difficult administration. Thus, there an urgent need develop drugs that...
ABSTRACT We report on the antiproliferative effects and ultrastructural biochemical alterations induced in vitro by 22,26-azasterol, a sterol Δ 24(25) -methyltransferase (24-SMT) inhibitor, Leishmania amazonensis . When promastigotes amastigotes were exposed to 100 nM complete growth arrest cell lysis ensued after 72 (promastigotes) or 120 (amastigotes) h. Exposure of parasites this azasterol led depletion parasite endogenous sterols (episterol 5-dehydroepisterol) their replacement...
This paper describes the synthesis of some novel azasterols based on (20R,22ξ)-5α-pregnan-20-(piperidin-2-yl)-3β,20-diol. These compounds are potential inhibitors enzyme sterol 24-methyltransferase (24-SMT), which is a vital in biosynthesis ergosterol and related 24-alkyl sterols. Structure−activity studies were undertaken to understand important features for activity against enzyme, with aim increasing selectivity. The evaluated inhibition recombinant Leishmania major 24-SMT effect...
ABSTRACT This paper describes the design and evaluation of novel azasterols as potential compounds for treatment leishmaniasis other diseases caused by trypanosomatid parasites. Azasterols are a known class ( S )-adenosyl- l -methionine: Δ 24 -sterol methyltransferase(24-SMT) inhibitors in fungi, plants, some parasitic protozoa. The prepared showed activity at micromolar nanomolar concentrations when tested against Leishmania spp. Trypanosoma enzymatic sterol composition studies indicated...
ER-119884 and E5700, novel arylquinuclidine derivatives developed as cholesterol-lowering agents, were potent in vitro growth inhibitors of both proliferative stages Leishmania amazonensis, the main causative agent cutaneous leishmaniasis South America, with 50% inhibitory concentrations (IC(50)s) being low-nanomolar to subnanomolar range. The compounds very noncompetitive native L. amazonensis squalene synthase (SQS), inhibition constants also nanomolar Growth was strictly associated...
Parasitic protozoa are important agents of human and animal diseases in Brazil around the world. Protozoan parasites Leishmania genus causative agent leishmaniasis, one most neglected tropical as designated by World Health Organization (WHO). Leishmaniasis affects about 12 million people worldwide can be divided into following three main clinical manifestations: cutaneous, mucocutaneous, visceral leishmaniasis [1].
Background The neglected human diseases caused by trypanosomatids are currently treated with toxic therapy limited efficacy. In search for novel anti-trypanosomatid agents, we showed previously that the Crotalus viridis (Cvv) snake venom was active against infective forms of Trypanosoma cruzi. Here, describe purification crovirin, a cysteine-rich secretory protein (CRISP) from Cvv promising activity trypanosomes and Leishmania. Methodology/Principal Findings Crude extract loaded onto reverse...
Amiodarone (AMIO), the most frequently antiarrhythmic drug used for symptomatic treatment of chronic Chagas' disease patients with cardiac compromise, has recently been shown to have also specific activity against fungi, Trypanosoma cruzi and Leishmania . In this work, we characterized effects AMIO on proliferation, mitochondrial physiology, ultrastructure amazonensis promastigotes intracellular amastigotes. The IC 50 values were 4.21 0.46 <mml:math...
Autophagy is a central process behind the cellular remodeling that occurs during differentiation of Leishmania, yet cargo protozoan parasite's autophagosome unknown. We have identified glycosomes, peroxisome-like organelles uniquely compartmentalize glycolytic and other metabolic enzymes in Leishmania kinetoplastid parasitic protozoa, as cargo. It has been proposed number glycosomes their content change life cycle key adaptation to different environments encountered. Quantification...
ABSTRACT Leishmaniases comprise a spectrum of diseases caused by protozoan parasites the Leishmania genus. Treatments available have limited safety and efficacy, high costs, difficult administration. Thus, there is an urgent need for safer more-effective therapies. Most trypanosomatids essential requirement ergosterol other 24-alkyl sterols, which are absent in mammalian cells. In previous studies, we showed that amazonensis highly susceptible to aryl-quinuclidines, such as E5700, inhibit...
Leishmaniasis, one of the most significant neglected diseases around world, is caused by protozoan parasites Leishmania genus. Nowadays, available aetiological treatments for leishmaniasis have variable effectiveness and several problems such as serious side effects, toxicity, high cost an increasing number resistance cases. Thus, there urgent need safe, oral cost-effective drugs leishmaniases. Previously, our group has shown effect ergosterol biosynthesis inhibitors on amazonensis. Herein,...
SUMMARY Leishmaniasis is a set of clinically distinct infectious diseases caused by Leishmania , genus flagellated protozoan parasites, that affects ∼12 million people worldwide, with ∼2 new infections annually. Plants are known to produce substances defend themselves against pathogens and predators. In the Lycopersicon which includes tomato, L. esculentum main antimicrobial compound steroidal glycoalkaloid α -tomatine. The loss saccharide side-chain tomatine yields aglycone tomatidine....
Acidocalcisomes are acidic calcium stores found in diverse organisms, being conserved from bacteria to humans. They possess an matrix that contains several cations bound phosphates, which mainly present the form of short and long polyphosphate chains. Their is acidified through action proton pumps such as a vacuolar ATPase pyrophosphatase. Calcium uptake occurs Ca2+/H+ countertransporting located membrane organelle. have been identified variety microorganisms, including Apicomplexan...
There is an urgent need for the development of new drugs treatment tropical parasitic diseases such as Chagas' disease and leishmaniasis. One potential drug target in organisms that cause these sterol biosynthesis. This paper describes design synthesis quinuclidine derivatives inhibitors a key enzyme biosynthesis, squalene synthase (SQS). A number compounds were recombinant Leishmania major SQS at submicromolar concentrations discovered. Some also selective parasite rather than homologous...
Abstract Background Although Candida species are commensal microorganisms, they can cause many invasive fungal infections. In addition, antifungal resistance contribute to failure of treatment. The purpose this study was evaluate the activity inhibitors Δ 24(25) -sterol methyltransferase (24-SMTI), 20-piperidin-2-yl-5α-pregnan-3β-20(R)-diol (AZA), and 24(R,S),25-epiminolanosterol (EIL), against clinical isolates spp., analysing ultrastructural changes. Results AZA EIL were found be potent...