A5056362500- Blood Coagulation and Thrombosis Mechanisms
- Venomous Animal Envenomation and Studies
- Protease and Inhibitor Mechanisms
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Venous Thromboembolism Diagnosis and Management
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Toxin Mechanisms and Immunotoxins
- Platelet Disorders and Treatments
- Blood properties and coagulation
- Complement system in diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Malaria Research and Control
- Research on Leishmaniasis Studies
- COVID-19 Clinical Research Studies
- Signaling Pathways in Disease
- SARS-CoV-2 and COVID-19 Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Trypanosoma species research and implications
- Immune cells in cancer
- Invertebrate Immune Response Mechanisms
- Bioactive Natural Diterpenoids Research
- Viral Infectious Diseases and Gene Expression in Insects
Universidade Federal do Rio de Janeiro
2015-2024
Centro Hospitalar do Baixo Vouga
2019-2022
Sanofi (Brazil)
2019
National Institute of Allergy and Infectious Diseases
2011-2012
National Institutes of Health
2011-2012
University of Lausanne
2012
Czech Academy of Sciences, Biology Centre
2012
Institute of Parasitology
2012
Universidade de São Paulo
2012
Universidade Federal do Triângulo Mineiro
2011
Abstract Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release tumor-derived extracellular vesicles and activation host vascular cells. It was that neutrophil traps (NETs) contribute prothrombotic phenotype in cancer. In this study, we evaluated possible cooperation between exosomes NETs thrombosis. Female BALB/c mice were orthotopically injected with 4T1 breast...
Exposure of phosphatidylserine (PS) on cellular membranes and membrane-derived microvesicles stimulates a number anti-inflammatory responses involved in malignant processes. Herein we show that B16F10 cells, highly metastatic melanoma cell line, produce large quantities PS-containing vitro. Tumor increased TGF-beta(1) production by cultured macrophages and, vivo, enhanced the potential cells C57BL/6 mice, both effects being reversed annexin V. Most strikingly, induced metastasis BALB/c which...
Neutrophil extracellular traps (NETs) have been associated with several steps of tumor progression, including primary growth and metastasis. One the key features for acquisition metastatic ability is epithelial-mesenchymal transition (EMT), a complex cellular program. In this study, we evaluated isolated NETs in modulating pro-metastatic phenotype human breast cancer cells. Tumor cells were treated then samples generated cell migration, quantitative RT-PCR, western blotting,...
RNA aptamers are synthetic oligonucleotide-based affinity molecules that utilize unique three-dimensional structures for their and specificity to a target such as protein. They hold the promise of numerous advantages over biologically produced antibodies; however, binding often insufficient successful implementation in diagnostic assays or therapeutic agents. Strong is important improve downstream applications. We report here use phosphorodithioate (PS2) substitution on single nucleotide...
Cancer patients are at increased risk of developing thrombosis, comorbidity that has been associated with neutrophil counts and the formation extracellular traps (NETs). Interleukin-1β (IL-1β) modulates expression granulocyte colony-stimulating factor (G-CSF), a cytokine promotes cancer-associated neutrophilia NET generation. Herein, we combined murine breast cancer model flow-restriction thrombosis to evaluate whether IL-1β blockade could interfere thrombosis. Mice bearing metastatic 4T1...
Accumulating evidence into the pathogenesis of COVID-19 highlights a hypercoagulability state with high risk life-threatening thromboembolic complications. However, mechanisms and their link to hyperinflammation remain poorly understood. Here, we investigate functions platelet activation platelet-monocyte interactions in inflammatory amplification during SARS-CoV-2 infection. We used combination immunophenotyping, single-cell analysis, functional assays, pharmacological approaches gain...
Blood-sucking arthropods' salivary glands contain a remarkable diversity of antihemostatics. The aim the present study was to identify unique anticoagulant sand fly Lutzomyia longipalpis, which remained elusive for decades.Several L. longipalpis proteins were expressed in human embryonic kidney 293 cells and screened inhibition blood coagulation. A novel 32.4-kDa molecule, named Lufaxin, identified as slow, tight, noncompetitive, reversible inhibitor factor Xa (FXa). Notably, Lufaxin's...
The identity of vampire bat saliva anticoagulant remained elusive for almost a century. Sequencing the salivary gland genes from Desmodus rotundus identified Desmolaris as novel 21.5-kDa naturally deleted (Kunitz 1-domainless) form tissue factor pathway inhibitor. Recombinant was expressed in HEK293 cells and characterized slow, tight, noncompetitive inhibitor (F) XIa by mechanism modulated heparin. also inhibits FXa with lower affinity, independently protein S. In addition, binds kallikrein...
Atazanavir (ATV) has already been considered as a potential repurposing drug to 2019 coronavirus disease (COVID-19); however, there are controversial reports on its mechanism of action and effectiveness anti-severe acute respiratory syndrome 2 (SARS-CoV-2). Through the pre-clinical chain experiments: enzymatic, molecular docking, cell-based in vivo assays, it is demonstrated here that both SARS-CoV-2 B.1 lineage variant concern gamma susceptible this antiretroviral. Enzymatic assays docking...
Summary Ixolaris is a two-Kunitz tick salivary gland protein identified in Ixodes scapularis that presents extensive sequence homology t TFPI.It binds to FXa or FX as scaffolds and inhibits tissue factor/ FVIIa complex (extrinsic Xnase). Differently from TFPI, ixolaris does not bind the active site cleft of FXa. Instead, comple formation mediated by heparin-binding exosite,which may also results decreased activity into prothrombi nase complex.In this report,we show recombinant 125I-ixo laris...
Summary Fucosylated chondroitin sulfate is a glycosaminoglycan from sea cucumber composed of sulfate-like core with branches sulfated fucose. This has high anticoagulant and antithrombotic activities. Its serpin-dependent activity mostly due to activating thrombin inhibition by heparin cofactor II. Here, we evaluated the fucosylated using antithrom-bin- II-free plasmas. In contrast mammalian heparin, invertebrate still able prolong coagulation time delay factor Xa generation in serpin-free...
Summary Shedding of microvesicles (MVs) by cancer cells is implicated in a variety biological effects, including the establishment cancer-associated hypercoagulable states. However, mechanisms underlying malignant transformation and acquisition procoagulant properties tumour-derived MVs are poorly understood. Here we investigated prothrombotic produced melanocyte-derived cell line (melan-a) as compared to its tumourigenic melanoma counterpart Tm1. Tumour exhibit two-fold higher rate...
// Vitor Hugo de Almeida 1, 2 , Isabella dos Santos Guimarães Lucas R. Almendra 1 Araci M.R. Rondon Tatiana M. Tilli 3 Andréia C. Melo Cinthya Sternberg 2, 4 and Robson Q. Monteiro Instituto Bioquímica Médica Leopoldo De Meis, Universidade Federal do Rio Janeiro, RJ, Brazil Divisão Pesquisa Clínica e Desenvolvimento Tecnológico, Nacional Câncer, Centro Tecnológico em Saúde, Fundação Oswaldo Cruz, Present...