A5067868520- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Analytical Chemistry and Chromatography
- Pesticide Residue Analysis and Safety
- SARS-CoV-2 detection and testing
- Radioactive Decay and Measurement Techniques
- SARS-CoV-2 and COVID-19 Research
- GABA and Rice Research
- Plant Physiology and Cultivation Studies
- Metabolomics and Mass Spectrometry Studies
- Analytical Methods in Pharmaceuticals
- Legume Nitrogen Fixing Symbiosis
- Leaf Properties and Growth Measurement
- Muscle metabolism and nutrition
- Mass Spectrometry Techniques and Applications
- Mycotoxins in Agriculture and Food
- Extraction and Separation Processes
- Spectroscopy and Chemometric Analyses
- Soil Carbon and Nitrogen Dynamics
- Plant Stress Responses and Tolerance
- Greenhouse Technology and Climate Control
- Glycogen Storage Diseases and Myoclonus
- Chemical and Physical Properties in Aqueous Solutions
- Soil and Water Nutrient Dynamics
- Membrane-based Ion Separation Techniques
Bureau international des poids et mesures
2020-2024
Soil Agro and Hydrosystems Spatialization
2017-2023
Institut Agro Rennes-Angers
2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2022
AgroParisTech
2011-2020
Institut Jean-Pierre Bourgin
2007-2020
Université Paris-Saclay
2017-2020
Laboratoire Ingénierie, Procédés, Aliments
2017-2018
Institut National de la Recherche Agronomique
2005-2013
Département Santé des Plantes et Environnement
2005-2006
ABSTRACT Nitrate is an important nitrogen source for plants, but also a signal molecule that controls various aspects of plant development. In the present study role nitrate on seed dormancy in Arabidopsis was investigated. The effects either mutations affecting reductase genes or different regimes mother plants seeds produced were analysed. Altogether, data show conditions favouring accumulation and lead to lower with little other morphological biochemical differences. Analysis germination...
Abstract Here, we report that SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER (SWEET16) from Arabidopsis (Arabidopsis thaliana) is a vacuole-located carrier, transporting glucose (Glc), fructose (Fru), and sucrose (Suc) after heterologous expression in Xenopus laevis oocytes. The SWEET16 gene, similar to the homologs gene SWEET17, mainly expressed vascular parenchyma cells. Application of Glc, Fru, or Suc, as well cold, osmotic stress, low nitrogen, provoke down-regulation messenger RNA...
In higher plants the glutamate dehydrogenase (GDH) enzyme catalyzes reversible amination of 2-oxoglutarate to form glutamate, using ammonium as a substrate. For better understanding physiological function GDH either in assimilation or supply 2-oxoglutarate, we used transgenic tobacco (Nicotiana tabacum L.) overexpressing two genes encoding enzyme. An vivo real time (15)N-nuclear magnetic resonance (NMR) spectroscopy approach allowed demonstration that, when were overexpressed individually...
Our understanding of plant growth in response to nitrogen (N) supply is mainly based on studies mutants and transformants. This study explored the natural variability Arabidopsis thaliana first find out its global N availability secondly characterize plasticity for metabolism among 23 genetically distant accessions under normal (N+), limited (N–), starved (N0) supplies. Plant was estimated by eight morphological traits characterizing shoot root 10 metabolic parameters that represented carbon...
Glutamate dehydrogenase (GDH; EC 1.4.1.2) is able to carry out the deamination of glutamate in higher plants. In order obtain a better understanding physiological function GDH leaves, transgenic tobacco (Nicotiana tabacum L.) plants were constructed that overexpress two genes from Nicotiana plumbaginifolia (GDHA and GDHB under control Cauliflower mosiac virus 35S promoter), which encode α- β-subunits individually or simultaneously. plants, protein accumulated mitochondria mesophyll cells...
Main text The CCQM-K148.b comparison, undertaken with a parallel pilot study CCQM-P187.b, was coordinated by the BIPM and UME on behalf of Consultative Committee for Amount Substance: Metrology in Chemistry Biology (CCQM) Working Group Organic Analysis (OAWG). It National Measurement Institutes (NMIs) Designated (DIs) which provide measurement services organic analysis under CIPM Mutual Recognition Arrangement (MRA) designated Track A comparison within OAWG implementation CCQM Strategy...
Main text The CCQM-K179 comparison, undertaken with a parallel pilot study CCQM-P224, was coordinated by the BIPM and UME on behalf of Consultative Committee for Amount Substance: Metrology in Chemistry Biology (CCQM) Working Group Organic Analysis (OAWG). It National Measurement Institutes (NMIs) Designated (DIs) which provide measurement services organic analysis under CIPM Mutual Recognition Arrangement (MRA) designated Track C comparison within OAWG implementation CCQM Strategy Programme...
Although the physiological role of enzyme glutamate dehydrogenase which catalyses in vitro reversible amination 2-oxoglutarate to remains be elucidated, it is now well established that higher plants preferentially occurs mitochondria phloem companion cells. The Nicotiana plumbaginifolia and Arabidopis thaliana encoded by two distinct genes encoding either an alpha- or a beta-subunit. Using antisense mutants impaired expression genes, we showed leaves stems both beta-subunits are targeted In...
In eukaryotes, autophagy, a catabolic mechanism for macromolecule and protein recycling, allows the maintenance of amino acid pools nutrient remobilization. For better understanding relationship between autophagy nitrogen metabolism, we studied transcriptional plasticity genes (ATG) in nine Arabidopsis accessions grown under normal nitrate starvation conditions. The status N metabolism was monitored by measuring relative expression 11 related to rosette leaves. variation coding enzymes...
A reference material for virus-like particles traceable to the International System of Units (Système d'Unités - SI) is reported. The addresses need developing standards benchmark gene delivery systems and help harmonize measurement approaches characterization testing. a major component synthetic polypeptide produced by state-of-the-art analytical chemistry methods used generate systems. purity profile evaluated highest metrological order demonstrating traceability SI. adds emerging toolkit...
A population of 50 independent transgenic lettuces transformed with a nitrate reductase coding sequence under the control 35S promoter was studied. None them showed significantly lower levels when compared untransformed plants, despite presence (NR) activity that derives from transgene in at least four transformants. No repercussion on total NR (endogenous+transgenic) detected these plants. Nevertheless, 28% transformants phenotypes characteristic general silencing genes as already described...
Abstract BACKGROUND Bioconversion broths are complex media with microorganisms that convert substrates into products in the presence of salts and nitrogen sources may release biomolecules. This paper deals impact bioconversion broth components on membrane‐based reactive extraction 3‐hydroxypropionic acid (3‐HP) by tri‐ n ‐octylamine (TOA) ‐decanol preparation for an situ product recovery. The focus here is influence 3‐HP concentration (0.5–10 g L −1 ), initial pH solution (3–5) proteins...
Main text Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P216, was coordinated by Chinese National Institute Metrology (NIM), Research Council Canada (NRC) and Bureau International des Poids et Mesures (BIPM). The global coronavirus pandemic has also led to increased focus on antibody quantitation methods. IgG are among immunoglobulins produced immune system provide protection against SARS-CoV-2....
Mineral nutrient availability and in particular nitrogen abundance has a huge impact on plant fitness yield, so that plants have developed sophisticated adaptive mechanisms to cope with environmental fluctuations. The vast natural variation existing among the individuals of single species constitutes great potential decipher complex traits such as use efficiency. By using accessions Arabidopsis thaliana differ for their pattern adaptation stress, we investigated response nitrate supplies...
Main text Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P216, was coordinated by Chinese National Institute Metrology (NIM), Research Council Canada (NRC) and Bureau International des Poids et Mesures (BIPM). Eleven Institutes or Designated BIPM participated in first phase study (Part 1). The purpose this to develop measurement capabilities for larger proteins using recombinant humanized IgG monoclonal...
The year 2020 will be remembered for the most pernicious epidemic of modern history. SARS-CoV-2 coronavirus has affected millions people worldwide and triggered an unprecedented race development diagnostic tests therapeutical vaccines. These technologies require befitting measurements that underpin reliability patient safety. level blood-borne antibodies against is correlated initially with extent infection subsequently degree immunity attained. This renders their accurate measurement...
Main text The CCQM-K154.b comparison was coordinated by the Bureau International des Poids et Mesures (BIPM) and Chinese National Institute of Metrology (NIM) on behalf Organic Analysis Working Group (OAWG) Comité Consultatif pour la Quantité de Matière (CCQM) for Measurement Institutes (NMIs) Designated (DIs) which provide measurement services in organic analysis under 'Comité Mesures' Mutual Recognition Arrangement (CIPM MRA) and/or have participated BIPM's Mycotoxin Capacity Building...
Main text The original CCQM-K154.b comparison was coordinated by the Bureau International des Poids et Mesures (BIPM) and Chinese National Institute of Metrology (NIM) on behalf Organic Analysis Working Group (OAWG) Comité Consultatif pour la Quantité de Matière (CCQM) for Measurement Institutes (NMIs) Designated (DIs) which provide measurement services in organic analysis under 'Comité Mesures' Mutual Recognition Arrangement (CIPM MRA) and/or have participated BIPM's Mycotoxin Capacity...