A5070678967- Mycotoxins in Agriculture and Food
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Phytochemical Studies and Bioactivities
- Plant tissue culture and regeneration
- Fungal Plant Pathogen Control
- Fungal and yeast genetics research
- Carcinogens and Genotoxicity Assessment
- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Genetically Modified Organisms Research
- Microbial Metabolic Engineering and Bioproduction
- Fermentation and Sensory Analysis
- Photosynthetic Processes and Mechanisms
- Heat shock proteins research
- Plant Disease Resistance and Genetics
- Potato Plant Research
- Insect and Pesticide Research
- Plant Stress Responses and Tolerance
- thermodynamics and calorimetric analyses
- Indoor Air Quality and Microbial Exposure
University of Parma
2012-2023
Consorzio Interuniversitario Reattività Chimica e Catalisi
2017
In higher plants the glutamate dehydrogenase (GDH) enzyme catalyzes reversible amination of 2-oxoglutarate to form glutamate, using ammonium as a substrate. For better understanding physiological function GDH either in assimilation or supply 2-oxoglutarate, we used transgenic tobacco (Nicotiana tabacum L.) overexpressing two genes encoding enzyme. An vivo real time (15)N-nuclear magnetic resonance (NMR) spectroscopy approach allowed demonstration that, when were overexpressed individually...
To develop a multiplex reverse transciption-polymerase chain reaction (RT-PCR) protocol to discriminate aflatoxin-producing from aflatoxin-nonproducing strains of Aspergillus flavus.The was first optimized on set obtained laboratory collections and then validated A. flavus isolated corn grains collected in the fields Po Valley (Italy). Five genes aflatoxin gene cluster flavus, two regulatory (aflR aflS) three structural (aflD, aflO aflQ), were targeted with specific primers highlight their...
Glutamate dehydrogenase (GDH; EC 1.4.1.2) is able to carry out the deamination of glutamate in higher plants. In order obtain a better understanding physiological function GDH leaves, transgenic tobacco (Nicotiana tabacum L.) plants were constructed that overexpress two genes from Nicotiana plumbaginifolia (GDHA and GDHB under control Cauliflower mosiac virus 35S promoter), which encode α- β-subunits individually or simultaneously. plants, protein accumulated mitochondria mesophyll cells...
NAD-dependent glutamate dehydrogenase (NAD-GDH) of higher plants has a central position at the interface between carbon and nitrogen metabolism due to its ability carry out deamination glutamate. In order obtain better understanding physiological function NAD-GDH under salt stress conditions, transgenic tobacco ( Nicotiana tabacum L.) that overexpress two genes from plumbaginifolia individually GDHA GDHB ) or simultaneously GDHA/B were grown in presence 50 mM NaCl. different GDH...
Abstract The issue of food contamination by aflatoxins presently constitutes a social emergency, since they represent severe risk for human and animal health. On the other hand, use pesticides has to be contained, this generates long term residues in environment. Here we present synthesis series chelating ligands based on thiosemicarbazone scaffold, evaluated their antifungal antiaflatoxigenic effects. Starting from molecules natural origin known properties, introduced thio- group then...
Urban airborne particulate is a complex mixture of air pollutants, many which have not been identified. However, short-term mutagenesis tests together with chemicophysical parameter analysis are able to better assess quality and genotoxic load. The findings continuous monitoring (January 1991-August 1998) urban genotoxicity Po Valley town (Italy) on Salmonella typhimurium Saccharomyces cerevisiae reported. During this period, various measures (catalytic devices, unleaded fuels, annual...
Although the physiological role of enzyme glutamate dehydrogenase which catalyses in vitro reversible amination 2-oxoglutarate to remains be elucidated, it is now well established that higher plants preferentially occurs mitochondria phloem companion cells. The Nicotiana plumbaginifolia and Arabidopis thaliana encoded by two distinct genes encoding either an alpha- or a beta-subunit. Using antisense mutants impaired expression genes, we showed leaves stems both beta-subunits are targeted In...
To develop a simple, high-throughput and inexpensive procedure to detect quantify aflatoxins into the culture media of growing mycelia.Fungal conidia (Aspergillus flavus) were inoculated wells microplate containing 200 μl different formulations coconut-derived liquid medium. Time-dependent production in was evaluated by relying on UV-induced fluorescence emission toxin, using reader. These data validated comparison with outputs conventional HPLC-based procedure. Determinations aflatoxin...
Many fungal genera such as Aspergillus, Penicillium, Fusarium and Alternaria are able to produce, among many other metabolites, the aflatoxins, a group of toxic carcinogenic compounds. To reduce their formation, synthetic fungicides used an effective way intervention. However, extensive use molecules generates long-term residues into food environment. The need new antifungal molecules, with high specificity low off-target toxicity is worth. aim this study was evaluate: i) genotoxicity newly...
Great are the expectations for a new generation of antimicrobials, and strenuous research efforts towards exploration diverse molecular scaffolds-possibly natural origin - aimed at synthesis compounds against spread hazardous fungi. Also high but winding paths leading to definition biological targets specifically fitting drug's structural characteristics. The present study is addressed inspect differential behaviours cinnamaldehyde benzaldehyde thiosemicarbazone scaffolds, exploiting...
A Penicillium nordicum strain previously assessed for its atoxigenicity was tested against a toxigenic of the same species on salami, in order to assess effectiveness as biocontrol agent OTA containment. Sixty salami were inoculated with different combinations P. OTA−/OTA+ suspensions and ripened under controlled thermohygrometric conditions. After 7, 18, 29, 40 days, both fungal counts chemical analyses carried out casings. never found used control, while it occasionally detected traces...