A5089404192- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Protein purification and stability
- Analytical chemistry methods development
- Biosensors and Analytical Detection
- Analytical Chemistry and Chromatography
- Atmospheric aerosols and clouds
- Microfluidic and Capillary Electrophoresis Applications
- Atmospheric Ozone and Climate
- Atmospheric chemistry and aerosols
- Chemical Synthesis and Analysis
- Analytical Chemistry and Sensors
- Advanced Proteomics Techniques and Applications
- Allergic Rhinitis and Sensitization
- Luminescence and Fluorescent Materials
- Advanced Chemical Sensor Technologies
- Pharmaceutical and Antibiotic Environmental Impacts
- Calibration and Measurement Techniques
- Photonic and Optical Devices
- Aquatic Ecosystems and Phytoplankton Dynamics
- Trace Elements in Health
- Marine and coastal ecosystems
- Crystallization and Solubility Studies
Federal Institute For Materials Research and Testing
2016-2025
Humboldt-Universität zu Berlin
2024-2025
Reutlingen University
2021
Leibniz Institute for High Performance Microelectronics
2021
Technical University of Applied Sciences Wildau
2021
Richard Wolf (Germany)
2012
Technical University of Munich
1998-2008
German Meteorological Service
1985-2008
Nanjing University
2008
BOKU University
2005
Air pollution and climate change are potential drivers for the increasing burden of allergic diseases. The molecular mechanisms by which air pollutants parameters may influence diseases, however, complex elusive. This article provides an overview physical, chemical biological interactions between pollution, change, allergens, adjuvants immune system, addressing how these promote development allergies. We reviewed synthesized key findings from atmospheric, climate, biomedical research....
Introduction Atherosclerosis is the underlying cause of multiple cardiovascular pathologies. The present-day clinical imaging modalities do not offer sufficient information on plaque composition or rupture risk. A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) a strongly upregulated proteoglycan-cleaving enzyme that specific to diseases, inter alia, atherosclerosis. Materials Methods Male apolipoprotein E-deficient mice received high-fat diet for 2 (n = 11) months...
A parallel affinity sensor array (PASA) for the rapid automated analysis of 10 antibiotics in milk is presented, using multianalyte immunoassays with an indirect competitive ELISA format. Microscope glass slides modified (3-glycidyloxypropyl)trimethoxysilane were used preparation hapten microarrays. Protein conjugates haptens immobilized as spots on disposable chips, which processed a flow cell. Monoclonal antibodies against penicillin G, cloxacillin, cephapirin, sulfadiazine,...
The rapid temperature increase of 1°C over mainland Europe since 1980 is considerably larger than the rise expected from anthropogenic greenhouse gas increases. Here we present aerosol optical depth measurements six specific locations and surface irradiance a large number radiation sites in Northern Germany Switzerland. show decline concentration up to 60%, which have led statistically significant solar under cloud‐free skies 1980s. confirm brightening that direct effect had an approximately...
The effects of air pollution on allergic diseases are not yet well-understood. Here, we show that proteins, in particular birch pollen proteins including the allergen Bet v 1, efficiently nitrated by polluted air. This posttranslational modification is likely to trigger immune reactions and provides a molecular rationale for promotion allergies traffic-related pollution. Enzyme immunoassays have been used determine equivalent degrees nitration (EDN) protein samples exposed urban outdoor...
An automated optical biosensor system based on fluorescence excitation and detection in the evanescent field of a quartz fiber was used to detect 16-mer oligonucleotides DNA hybridization assays. A biotinylated capture probe immobilized surface via avidin or streptavidin. The with fluorescein-labeled complementary strands monitored real time by detection. double formed could be dissociated chemical thermal regeneration, allowing one perform hundreds assay cycles same fiber. signal loss...
A new class of boron−dipyrromethene (BDP or BODIPY) dyes was obtained by phenanthrene fusion to the β-pyrrole positions, absorbing in wavelength range important laser sources. Despite a 'propeller-like' distorted structure crystalline state, chromophore absorbs (log ε ≥ 5) and fluoresces (Φf 0.8) strongly can be easily turned into fluorescence light-up probe. Incorporation latex beads produces bright photostable single-dye Förster Resonance Energy Transfer (FRET) particles for microscopy...
Hydroxyl radical-induced oxidation of proteins and peptides can lead to the cleavage peptide, leading a release fragments. Here, we used high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) pre-column online ortho-phthalaldehyde (OPA) derivatization-based amino acid analysis by HPLC with diode array detection fluorescence identify quantify free acids released upon hydroxyl radicals. Bovine serum albumin (BSA), ovalbumin (OVA) as model proteins, synthetic tripeptides...
Fast and accurate determination of the protein content a sample is an important non-trivial task many biochemical, biomedical, food chemical, pharmaceutical, environmental research activities. Different methods total are used for wide range proteins with highly variable properties in complex matrices. These usually work reasonably well under controlled conditions, but results non-standard samples often questionable. Here, we compare new well-established methods, including traditional amino...
Abstract Monoclonal antibodies are biotechnologically produced proteins with various applications in research, therapeutics and diagnostics. Their ability to recognize bind specific molecule structures makes them essential research tools therapeutic agents. Sequence information of is helpful for understanding antibody–antigen interactions ensuring their affinity specificity. De novo protein sequencing based on mass spectrometry a valuable method obtain the amino acid sequence peptides...
<i>Background:</i> Recent investigations have shown that proteins, including Bet v 1a, are nitrated by exposure to polluted urban air. We investigated immunogenic and allergenic properties of in vitro allergens vivo models. <i>Methods:</i> Untreated samples ovalbumin or 1a were compared for their ability stimulate proliferation cytokine secretion splenocytes from DO11.10 sensitized BALB/c mice, induce specific immunoglobulin (Ig)G1, IgG2a IgE mice. Additionally, sera...
Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in field diagnostics. In order to enable rapid and accurate measurement cTnI with potential online measurements, a chemiluminescence-based immunosensor is presented proof concept. A flow cell was designed combined sensitive CMOS camera allowing optical readout. addition,...
Homochirality is an obvious feature of life on Earth. On the other hand, extraterrestrial samples contain largely racemic compounds. The same true for any common organic synthesis. Therefore, it has been a perplexing puzzle decades how these racemates could have formed enantiomerically enriched fractions as basis origin homochiral forms. Numerous hypotheses put forward to preferentially molecules and accumulated In this article, shown that homochirality abiotic pool at time formation first...
The described in vitro test system for allergy diagnosis is based on microscope glass slides activated with (3-glycidyloxypropyl)trimethoxysilane. Allergen solutions are immobilized as small droplets (approximately 10 nL) the a piezoelectric arrayer. In contrast to other tests specific IgE, such Pharmacia CAP FEIA, AlaSTAT, or FAST, only 25-microL serum sample needed screening of allergen-specific IgE against multitude allergens and can be performed less than 1 h. Compared multiallergen...
Environmental pollutants like fine particulate matter can cause adverse health effects through oxidative stress and inflammation. Reactive oxygen nitrogen species (ROS/RNS) such as peroxynitrite chemically modify proteins, but the of modifications on immune system human are not well understood. In course inflammatory processes, Toll-like receptor 4 (TLR4) sense damage-associated molecular patterns (DAMPs). Here, we investigate how TLR4 response pro-inflammatory potential proteinous DAMPs...
Peptide pools are important research tools in different biomedical fields. They consist of a complex mixture defined peptides, which places high demands on the production and quality control these products. Previously it has been shown that combination UHPLC with high-resolution mass-spectrometry (HRMS) is fast powerful method to confirm relative concentration structural identity all peptides expected be pool. In this work, additional information contained UV chromatograms mass spectra used...