A5012134871- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Bioactive Natural Diterpenoids Research
- SARS-CoV-2 and COVID-19 Research
- Hemoglobin structure and function
- Protein Structure and Dynamics
- Phytochemistry and Bioactive Compounds
- Viral Infections and Outbreaks Research
- Influenza Virus Research Studies
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Phytochemistry and Bioactivity Studies
- Animal Virus Infections Studies
Universidade Federal do Rio de Janeiro
2014-2023
National Education and Research Network
2021-2022
National Institute of Science and Technology for Structural Biology and Bioimaging
2021
Dengue virus (DENV) infection affects millions of people and is becoming a major global disease for which there no specific available treatment. pep14-23 recently designed peptide, based on conserved segment DENV capsid (C) protein. It inhibits the interaction C with host intracellular lipid droplets (LDs), crucial viral replication. Combining bioinformatics biophysics, here, we analyzed structure ability to bind different phospholipids, relating that information full-length C. We show...
The latex of Euphorbia tirucalli L. (Aveloz) is popularly used to treat cancers from diverse sources such as: breast, prostate, lung and kidney. Furthermore, high dilutions this (latex-HDs) have been successfully in the treatment tumors, although mechanisms involved antitumoral activity are not yet known. present study aimed investigate cytotoxic effects latex-HDs 2 human cell lines, including non-tumoral melanocytes (MelanA line) breast cancer cells (MCF7 line). Latex-HDs were obtained...
Nucleocapsid (NC) assembly is an essential step of the virus replication cycle. It ensures genome protection and transmission among hosts. Flaviviruses are human viruses for which envelope structure well known, whereas no information on NC organization available. Here we designed a dengue capsid protein (DENVC) mutant in highly positive spot conferred by arginine 85 α4-helix was replaced cysteine residue, simultaneously removing charge restricting intermolecular motion through formation...
Abstract Understanding protein structure and dynamics, which govern key cellular processes, is crucial for basic applied research. Intrinsically disordered (IDP) regions display multifunctionality via alternative transient conformations, being players in disease mechanisms. IDP are abundant, namely small viruses, allowing a large number of functions out proteome. The relation between function thus now seen from different perspective: as enable structural arrangements, each conformer can play...
Zika virus (ZIKV) became an important public health concern because infection was correlated to the development of microcephaly and other neurological disorders. Although structure virion has been determined by cryo-electron microscopy, information about nucleocapsid is lacking. We used nuclear magnetic resonance determine solution dynamics full length ZIKV capsid protein (ZIKVC). most structured as described for proteins Dengue West Nile viruses truncated ZIKVC (residues 23–98), here we...
Dengue virus (DENV) causes a major arthropod-borne viral disease, with 2.5 billion people living in risk areas. DENV consists 50 nm-diameter enveloped particle which the surface proteins are arranged icosahedral symmetry, while information about nucleocapsid (NC) structural organization is lacking. NC composed of genome, positive-sense single-stranded RNA, packaged by capsid (C) protein. Here, we established conditions for reproducible vitro assembly nucleocapsid-like particles (NCLPs) using...
ABSTRACT The SARS-CoV-2 nucleocapsid protein (N) is a multifunctional promiscuous nucleic acid-binding protein, which plays major role in assembly and discontinuous RNA transcription, facilitating the template switch of transcriptional regulatory sequences (TRS). Here, we dissect structural features N N-terminal domain (N-NTD), either with or without SR-rich motif (SR), upon binding to single double-stranded TRS DNA, as well their activities for dsTRS melting TRS-induced liquid-liquid phase...