A5029917690- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Diet, Metabolism, and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Synthesis and biological activity
- Endoplasmic Reticulum Stress and Disease
- Diet and metabolism studies
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- Liver Disease Diagnosis and Treatment
- Endometriosis Research and Treatment
- Click Chemistry and Applications
- Calcium signaling and nucleotide metabolism
- Reproductive System and Pregnancy
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Cancer therapeutics and mechanisms
- Plant tissue culture and regeneration
- Toxin Mechanisms and Immunotoxins
- Uterine Myomas and Treatments
- Proteoglycans and glycosaminoglycans research
- Growth Hormone and Insulin-like Growth Factors
Universidade Federal do Rio de Janeiro
2016-2025
Université Laval
2022
Pontificia Universidad Católica de Chile
2020
National Council for Scientific and Technological Development
2000-2004
Financiadora de Estudos e Projetos
2000-2004
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2000-2004
Hospital Universitário Clementino Fraga Filho
2000
Cancer cells are highly dependent on glycolysis to supply the energy and intermediates required for cell growth proliferation. The enzyme 6-phosphofructo-1-kinase (PFK) is critical glycolysis, its activity directly correlated with cellular glucose consumption. Resveratrol a potential anti-tumoral drug that decreases metabolism viability in cancer cells. However, mechanism involved resveratrol-mediated anti-tumor not entirely clear. In this work, it demonstrated resveratrol viability,...
Although produced by several types of tumours, the role serotonin on cancer biology is yet to be understood.The effects (5-HT) human breast cells proliferation, signalling pathways and metabolic profile were evaluated cytometry, western blotting, qPCR, enzymology confocal microscopy.Our results revealed that incubation MCF-7 with 10 µM 5-HT increased cell growth rate 28%, an effect was prevented 5-HTR2A/C antagonist, ketanserin. Conversely, increasing concentrations promoted glucose...
For a long period lactate was considered as dead-end product of glycolysis in many cells and its accumulation correlated with acidosis cellular tissue damage. At present, the role several physiological processes has been investigated based on properties an energy source, signalling molecule essential for repair. It is noteworthy that alters glycolytic flux independently from medium acidification, thereby this compound can regulate glucose metabolism within cells. PFK...
Background Clotrimazole is an azole derivative with promising anti-cancer effects. This drug interferes the activity of glycolytic enzymes altering their cellular distribution and inhibiting activities. The aim present study was to analyze effects clotrimazole on growth pattern breast cancer cells correlating metabolic profiles. Methodology/Principal Findings Three cell lines derived from human tissue (MCF10A, MCF-7 MDA-MB-231) that increasingly aggressive profiles were used. induces a...
Abstract The Western diet (high in fat and sucrose) consumption is a highly prevalent feature the whole world, mainly due to increasing of ultra-processed foods (UPF), which are cheaper easier-to-eat, as compared fresh nutritive meals. Epidemiological studies have associated UPF with development obesity, non-alcoholic liver disease (NAFLD) insulin resistance. For molecular studies, mice fed diets been used characterize signaling pathways involved these diet-induced pathologies. However,...
Abstract Melanoma is the most aggressive and fatal type of skin cancer due to being highly proliferative. Acetylsalicylic acid (ASA; Aspirin) salicylic (SA) are ancient drugs with multiple applications in medicine. Here, we showed that ASA SA present anticancer effects against a murine model implanted melanoma. These were also validated 3D- 2D-cultured melanoma B16F10 cells, where promoted pro-apoptotic effects. In both vivo vitro models, triggered endoplasmic reticulum (ER) stress, which...
Metabolic alterations are related to tumorigenesis and other age-related diseases that accelerated by "Westernized" diets. In fact, hypercaloric nutrition is associated with an increased incidence of cancers faster aging. Conversely, lifespan-extending strategies, such as caloric restriction, impose beneficial effects on both processes. Here, we investigated the metabolic consequences hypercaloric-induced aging tumor growth in female mice. Our findings indicate a high-fat high-sucrose diet...
Glycolytic enzymes reversibly associate with the human erythrocyte membrane (EM) as part of their regulatory mechanism. The site for this association has been described amino terminus band 3, a transmembrane anion transporter. Binding glycolytic to is recognized inhibit glycolysis, since binding inhibits catalytic activity these enzymes, including rate-limiting enzyme 6-phosphofructo-1-kinase (PFK). However, existence putative stimulatory within EM proposed. PFK able other proteins, such...
The present work describes the effects of metformin on hexokinase (HK) and phosphofructokinase (PFK) activities localization in different tissues from streptozotocin-induced diabetic mice. Diabetic mice lower HK PFK (50%) skeletal muscle, liver adipose tissue, as compared with control (P<0.05). Treatment 250 mg/kg reverses this pattern enzyme inhibition concomitant reversal hyperglycemia hypolactacidemia. Furthermore, treatment increases cytoskeleton-associated activity muscle; has been...
Abstract Diabetes mellitus is characterized by hyperglycemia and its associated complications, including cardiomyopathy. Metformin, in addition to lowering blood glucose levels, provides cardioprotection for diabetic subjects. Glycolysis essential cardiac metabolism reduction may contribute Hexokinase (HK) phosphofructokinase (PFK), rate‐limiting enzymes of glycolysis, are downregulated muscle from subjects, playing a central role on the decreased utilization heart Thus, aim this study was...
// Raquel G. Coelho 1, 2, 5 , Isadora C. Calaça 1 Deborah M. Celestrini Ana Helena P. Correia-Carneiro 3 Mauricio Costa Patricia Zancan 4 Mauro Sola-Penna Laboratório de Enzimologia e Controle do Metabolismo (LabECoM), Departamento Biotecnologia Farmacêutica, Faculdade Farmácia, Universidade Federal Rio Janeiro, Brasil 2 Instituto Bioquímica Médica Leopoldo De Meis, Hospital Universitário Clementino Fraga Filho, Oncobiologia Molecular...
The aim of this study was to evaluate the antidiabetic potential a leaf extract and flavonoids from Sedum dendroideum (SD). Additionally, our goals were establish possible structure/activity relationship between these assess most active flavonoid on glycolytic enzyme 6-phosphofructo-1-kinase (PFK). SD juice (LJ), flavonoid-rich fraction (BF), separately five evaluated intraperitoneally for their acute hypoglycemic activity in normal streptozotocin-induced diabetic mice. First, major...
Among the principal causative factors for development of complications related to aging is a diet rich in fats and sugars, also known as Western diet. This advocates numerous changes that might increase susceptibility initiate cancer and/or create tissue microenvironment more conducive growth malignant cells, thus favoring progression metastasis. Hypercaloric diets general lead oxidative stress generating reactive oxygen species induce endoplasmic reticulum stress. Our results demonstrate...
Fucosylated chondroitin sulfate is a glycosaminoglycan from sea cucumber, made up of alternating β-d-glucuronic acid and N-acetyl-β-d-galactosamine units, like mammalian sulfate. But the residues have branches sulfated fucose, which confer high anticoagulant antithrombotic properties on this compound. We now compared anticoagulant, bleeding effects fucosylated its carboxyl-reduced derivative. Both compounds similar action, mostly due to acceleration thrombin inhibition in presence heparin...
Influenza viruses cause highly contagious acute respiratory illnesses with significant mortality, especially among young children, elderly people, and individuals serious medical conditions. This encourages the development of new treatments for human flu. Biotherapies are diluted solutions prepared from biological products compounded following homeopathic procedures. To develop a biotherapy infectious influenza A virus (A/Aichi/2/68 H3N2) to verify its in vitro response. The ultradiluted...