A5081554162- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Heat shock proteins research
- Protein Hydrolysis and Bioactive Peptides
- Prion Diseases and Protein Misfolding
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- RNA Research and Splicing
- vaccines and immunoinformatics approaches
- Toxin Mechanisms and Immunotoxins
- Lipid Membrane Structure and Behavior
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Immune Response and Inflammation
- Science and Education Research
- Enzyme Structure and Function
- Genetics, Bioinformatics, and Biomedical Research
- Probiotics and Fermented Foods
- ATP Synthase and ATPases Research
- HIV Research and Treatment
- Invertebrate Immune Response Mechanisms
- Polydiacetylene-based materials and applications
- Bacteriophages and microbial interactions
- thermodynamics and calorimetric analyses
- Aquaculture disease management and microbiota
Universidade Estadual de Campinas (UNICAMP)
2022-2024
Universidade Federal de Goiás
2015-2020
Universidade Federal do Rio de Janeiro
2019
Abstract Plants are extensively used in traditional medicine, and several plant antimicrobial peptides have been described as potential alternatives to conventional antibiotics. However, after more than four decades of research no peptide is currently for treating bacterial infections, due their length, post-translational modifications or high dose requirement a therapeutic effect . Here we report the design derived from guava glycine-rich using genetic algorithm. This approach yields...
Attempts to isolate novel antimicrobial peptides from microbial sources have been on the rise recently, despite their low efficacy in therapeutic applications. Here, we report identification and characterization of a new efficient peptide bacterial strain designated A3 that exhibited highest identity with Paenibacillus ehimensis. Upon purification subsequent molecular peptide, referred as penisin, found be bacteriocin-like peptide. Consistent these results, RAST analysis entire genome...
Structural conversion of cellular prion protein (PrPC) into scrapie PrP (PrPSc) and subsequent aggregation are key events associated with the onset transmissible spongiform encephalopathies (TSEs). Experimental evidence supports role nucleic acids (NAs) in assisting this conversion. Here, we asked whether undergoes liquid-liquid phase separation (LLPS) if process is modulated by NAs. To end, two 25-mer DNA aptamers, A1 A2, were selected against globular domain recombinant murine (rPrP90-231)...
Due to the growing concern about antibiotic-resistant microbial infections, increasing support has been given new drug discovery programs. A promising alternative counter bacterial infections includes antimicrobial peptides (AMPs), which have emerged as model molecules for rational design strategies. Here we focused on study of Pa-MAP 1.9, a rationally designed AMP derived from polar fish Pleuronectes americanus. 1.9 was active against Gram-negative planktonic bacteria and biofilms, without...
Multifunctional and promiscuous antimicrobial peptides (AMPs) can be used as an efficient strategy to control pathogens. However, little is known about the structural properties of plant AMPs without disulfide bonds. CD NMR were elucidate structure peptide Cn ‐AMP1, a disulfide‐free isolated from green coconut water. Data here reported shows that transitory could different according micro‐environment. In this regard, ‐AMP1 showed random coil in water environment α‐helical presence SDS‐d 25...
ABSTRACT Structural conversion of cellular prion protein (PrP C ) into scrapie PrP Sc and subsequent aggregation are key events for the onset Transmissible Spongiform Encephalopathies (TSEs). Experimental evidences support role nucleic acids (NAs) in assisting process. Here, we used SELEX methodology to identify two 25-mer DNA aptamers against globular domain recombinant murine (rPrP 90-231 ), namely A1 A2. High-affinity binding A2 rPrP was verified by ITC. Aptamers structure characterized...
Antimicrobial peptides are recognized candidates with pharmaceutical potential against epidemic emerging multi‐drug resistant bacteria. In this study, we use nuclear magnetic resonance spectroscopy and molecular dynamics simulations to determine the unknown structure evaluate interaction dodecylphosphatidylcholine (DPC) sodium dodecylsulphate (SDS) micelles three W 6 ‐Hylin‐a1 analogs antimicrobial (HyAc, HyK, HyD). The HyAc, HyD bound DPC all formed by a unique α ‐helix structure. Moreover,...
HSP70 chaperones play pivotal roles in facilitating protein folding, refolding, and disaggregation through their binding releasing activities. This intricate process is further supported by J-domain proteins (JDPs), also known as DNAJs or HSP40s, which can be categorized into classes A B. In yeast, these are represented Ydj1 Sis1, respectively. While both stimulate the ATPase activity of Ssa1 (yeast HSP70) J-domain, only class B JDPs possess unique ability to efficiently processes. The...
Abstract We employed NMR spectroscopy to investigate the structure and dynamics of class B J domain protein (JDP) S. cerevisiae (Sis1) complexed with an EEVD peptide HSP70. It is widely recognized that interactions between motif Sis1 play a crucial role in chaperone activity. Notably, deletion impairs ability bind HSP70, while leaving interaction A JDP Ydj1 HSP70 unaffected. Leveraging advantages NMR, which particularly suitable for studying transient interactions, we provide compelling...