A5034061803- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Mosquito-borne diseases and control
- Radiopharmaceutical Chemistry and Applications
- Cancer therapeutics and mechanisms
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Proteoglycans and glycosaminoglycans research
- Computational Drug Discovery Methods
- Viral Infections and Vectors
- Nanoparticle-Based Drug Delivery
- Bacteriophages and microbial interactions
- Virology and Viral Diseases
- Synthesis and Biological Evaluation
- Click Chemistry and Applications
- Biosensors and Analytical Detection
- Molecular Junctions and Nanostructures
- COVID-19 Impact on Reproduction
- Protein purification and stability
- Carbohydrate Chemistry and Synthesis
- Bioactive Compounds and Antitumor Agents
- Protein Structure and Dynamics
Fundação Oswaldo Cruz
2014-2024
Instituto de Tecnologia e Pesquisa
2019-2023
National Institutes of Health
2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
Universidade do Estado do Rio de Janeiro
2015-2019
Instituto Vital Brazil (Brazil)
2016
Universidade Estadual da Zona Oeste
2016
Universidade Federal Rural do Rio de Janeiro
2016
Agents able to bind tightly and selectively disease markers can greatly benefit diagnosis therapy. Aptamers are functional molecules, usually DNA or RNA oligonucleotides, with the appropriate sequence structure form a complex target molecule. MUC1 is well-known tumour marker present in variety of malignant tumours it has been interest for many years. In this work we report selection aptamers that high affinity selectivity peptides. Combinatorial chemistry techniques based on SELEX...
The majority of cancers arise from malignant epithelial cells. We report the design synthetic oligonucleotides (aptamers) that are only internalized by cancer cells and can be precisely activated light to kill such Specifically, phototoxic DNA aptamers were selected bind unique short O-glycan-peptide signatures on surface breast, colon, lung, ovarian pancreatic These antigens not present normal but routed through endosomal Golgi compartments cells, thus providing a focused mechanism for...
Targeted radiopharmaceuticals offer the possibility of improved tumor imaging and radiotherapy, with reduced side effects. A variety monoclonal antibodies antibody fragments have previously been successfully radiolabeled used in diagnostic targeted radiotherapy cancer. Many such shown to recognize well-characterized MUC1 marker recently clinical trials. Furthermore, a number chelators synthesized are currently as for therapy. We now report synthesis novel, cyclen-based ligand...
Aptamers are characterized by a rapid renal clearance leading to short in vivo circulating half-life. In order use aptamers as anticancer therapeutic agents, their exposure time the tumor has be enhanced via increasing residency bloodstream. A way achieve this goal is conjugating aptamer poly(ethylene glycol) (PEG). Herein, we present conjugation of bifunctionalized anti-MUC1 (NH(2)-AptA-SR) with (99m)Tc coordinating moiety MAG2 and either conventional branched PEG or comb-shaped PolyPEG...
Structural conversion of cellular prion protein (PrPC) into scrapie PrP (PrPSc) and subsequent aggregation are key events associated with the onset transmissible spongiform encephalopathies (TSEs). Experimental evidence supports role nucleic acids (NAs) in assisting this conversion. Here, we asked whether undergoes liquid-liquid phase separation (LLPS) if process is modulated by NAs. To end, two 25-mer DNA aptamers, A1 A2, were selected against globular domain recombinant murine (rPrP90-231)...
The critical role of inappropriate inflammation is becoming accepted in many diseases that affect man, including cardiovascular diseases, inflammatory and autoimmune disorders, neurodegenerative conditions, infection cancer.This review proposes cancer up-regulates the angiotensin II type 1 (AT1) receptor through systemic oxidative stress hypoxia mechanisms, thereby triggering chronic processes to remodel surrounding tissue subdue immune system. Based on current literature clinical studies...
The growth-inhibitory activities of an extensive series quaternized quino[4,3,2-kl]acridinium salts against tumor cell lines in vitro have been measured and their biological properties interpreted the light differential binding to different DNA isoforms. Selectivity for quadruplex stabilization by compounds were explored through array methods: UV absorption fluorescence emission spectroscopy, surface plasmon resonance, competition dialysis. Quadruplex interaction was further characterized...
The early and specific detection of tumors remains a barrier in oncology, especially cases such as the triple-negative breast cancer (TNBC). To address this gap, aptamers have found an important application recognition tumor biomarkers mucin 1 (MUC1). However, there are still some difficulties use aptamer, their rapid biological clearance makes drugs limited. In study, anti-MUC1 aptamer was used drug delivery system (DDS) for radioactive polymeric nanoparticle (NP) imaging TNBCs. Thus,...
Aptamers are known for their short in vivo circulating half-life and rapid renal clearance. Their conjugation to poly(ethylene glycol) (PEG) is a way improve residence the body. Two aptamers (AptD AptF), having disulfide protected thiol modification on 3′ end, have been conjugated maleimide activated PEGs of various molecular weights structures (linear PEG20; branched PEG20 40; PolyPEG17, 40, 60 kDa). The high yield coupling (70−80% most cases) could be achieved using immobilized...
Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease certain types of cancer. Antibodies, either polyclonal or monoclonal, exhibit specificity for distinct members the extended kallikrein family, KLK6, were developed. With exception KLK3/PSA, identification generation aptamers, potential new tools with improved characteristics demanded therapeutic...
Abstract Molecular modeling studies carried out with experimental DNA models the sequence d[AG 3 (T 2 AG ) ] suggest that introduction of a net positive charge onto side chain series fluorenone carboxamides can improve G‐quadruplex binding. The terminal morpholino moiety was replaced novel N ‐methylmorpholinium cation starting from two 4‐carboxamide compounds. A different substitution on ring also investigated and submitted to same quaternarization process. All compounds were analyzed for...
Abstract Since its emergence in late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been posing a serious threat to public health worldwide as the causative agent of disease 2019 (COVID-19). Now distributed pandemic pattern, this still lacks an effective drug treatment with low toxicity, leading pharmaceutical companies and research labs work against time find candidate molecule efficiently treat affected patients. Due well-known broad-spectrum antimicrobial activity...
Infections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, cellular humoral immunity crucial developing individual protection. Here, we aim investigate hybrid against triggered ChAadOx1 nCoV-19 vaccine in Brazilian cohort. We investigated immune response from vaccination naïve (noCOVID-19) previously infected individuals (COVID-19) analyzing levels...
Aptamers, short, single stranded oligonucleotide entities, have been developed in the past 15 years against a plethora of targets and for variety applications. These range from inhibition receptors enzymes to identification small molecules sensor applications, development targeted therapeutic design novel diagnostic imaging agents. Furthermore, aptamers designed that cover wide diseases, HIV tropical cancer inflammation. Their easy flexibility use manipulation, offers further potential. In...