A5045098466- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Virology and Viral Diseases
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Bacterial Infections and Vaccines
- COVID-19 epidemiological studies
- Long-Term Effects of COVID-19
- Systemic Lupus Erythematosus Research
- Vaccine Coverage and Hesitancy
- Hepatitis C virus research
- Dermatological and COVID-19 studies
- vaccines and immunoinformatics approaches
- Infant Nutrition and Health
- Infectious Encephalopathies and Encephalitis
- Malaria Research and Control
- Immune Response and Inflammation
- Primate Behavior and Ecology
- Diphtheria, Corynebacterium, and Tetanus
- Pneumonia and Respiratory Infections
- Chronic Lymphocytic Leukemia Research
- Parvovirus B19 Infection Studies
Fundação Oswaldo Cruz
2000-2025
Instituto de Tecnologia e Pesquisa
2020-2024
Secretaria Municipal de Saúde
2008
Lactoferrin (Lf) is an important immunomodulator in infections caused by different agents. During SARS-CoV-2 infection, Lf can hinder or prevent virus access to the intracellular environment. Severe cases of COVID-19 are related increased production cytokines, accompanied a weak type 1 interferon response.
In the last decade, Flaviviruses such as yellow fever (YFV) and Zika (ZIKV) have expanded their transmission areas. These viruses originated in Africa, where they exhibit both sylvatic interhuman cycles. Brazil, risk of YFV urbanization has grown, with approaching most densely populated metropolis, while concern about ZIKV spillback to a cycle risen. To investigate these health threats, we carried out extensive collections arbovirus screening 144 free-living, non-human primates (NHPs) 5219...
Zika virus (ZIKV) infections during pregnancy can result in Congenital Syndrome (CZS), a range of severe neurological outcomes fetuses that primarily occur early gestational stages possibly due to placental damage. Although some placentas maintain ZIKV persistence for weeks or months after the initial infection and diagnosis, impact this viral is still unknown. Here, we aimed investigate immunological repercussion term placentas. As such, from 64 pregnant women diagnosed with different...
Abstract Since its emergence in late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been posing a serious threat to public health worldwide as the causative agent of disease 2019 (COVID-19). Now distributed pandemic pattern, this still lacks an effective drug treatment with low toxicity, leading pharmaceutical companies and research labs work against time find candidate molecule efficiently treat affected patients. Due well-known broad-spectrum antimicrobial activity...
Infections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, cellular humoral immunity crucial developing individual protection. Here, we aim investigate hybrid against triggered ChAadOx1 nCoV-19 vaccine in Brazilian cohort. We investigated immune response from vaccination naïve (noCOVID-19) previously infected individuals (COVID-19) analyzing levels...
Host factors that influence Congenital Zika Syndrome (CZS) outcome remain elusive. Interferons have been reported as the main antiviral factor in and other flavivirus infections. Here, we accessed samples from 153 pregnant women (77 without 76 with CZS) 143 newborns 66 exposed to ZIKV conducted a case-control study verify whether interferon alfa receptor 1 (IFNAR1) lambda 2 4 (IFNL2/4) single nucleotide polymorphisms (SNPs) contribute CZS outcome, characterized placenta gene expression...
The yellow fever vaccine (YF17DD) is highly effective with a single injection conferring protection for at least 10 years. YF17DD induces polyvalent responses, TH1/TH2 CD4+ profile, robust T CD8+ and synthesis of interferon-gamma (IFN-γ), culminating in high titers neutralizing antibodies. Furthermore, C-type lectin domain containing 5A (CLEC5A) has been implicated innate outcomes other flaviviral infections. Here, we conducted follow-up study volunteers immunized YF17DD, investigating the...
Abstract The re-emergence of yellow fever (YF) urged new mass vaccination campaigns and, in 2017, the World Health Organization approved use fractional dose (FD) YF vaccine due to stock shortage. In an observational cross-sectional investigation, we have assessed viremia, antibodies, soluble mediators and effector memory T B-cells induced by primary volunteers with FD standard (SD). Similar viremia levels antibodies markers were early after immunization. However, a faster decrease latter was...
A randomized, double-blinded study evaluating the immunogenicity, safety and consistency of production a combined diphtheria-tetanus-pertussis-Haemophilus influenzae type b vaccine entirely produced in Brazil by Bio-Manguinhos Instituto Butantan (DTP/Hib-BM) was undertaken. The reference had same DTP but Hib component using purified materials supplied GlaxoSmithKline (DTP/Hib-GSK), which is registered has Brazilian National Immunization Program for over more than five years. One thousand...
One patient presented hyporexia, asthenia, adynamia, and jaundice two months after acute yellow fever (YF) onset; plus laboratory tests indicating hepatic cytolysis a rebound of alanine aspartate transaminases, total direct bilirubin levels. Laboratory discarded autoimmune hepatitis, inflammatory or metabolic liver disease, new infections caused by hepatotropic agents. Anti-YFV IgM, IgG neutralizing antibodies were detected in different times, but no viremia. A biopsy was collected three YF...
Abstract Background Alouatta spp. are highly susceptible to yellow fever (YF) infection and develop an often fatal disease. The threat posed by outbreak started in 2016 leads us investigate vaccination as a potential tool preventing YF non‐human primates (NHP). Methods Susceptible howler monkeys were immunized with three different concentrations of the human Brazilian commercial YF17DD vaccine. Post‐vaccination viremia/RNAemia, immunogenicity, safety characterized. Results vaccine did not...
Despite the rapid mass vaccination against COVID-19, emergence of new SARS-CoV-2 variants concern, such as omicron, is still a great distress, and therapeutic options are needed. Bovine lactoferrin (bLf), multifunctional iron-binding glycoprotein available in unsaturated (apo-bLf) saturated (holo-bLf) forms, has been shown to exert broad-spectrum antiviral activity many viruses. In this study, we evaluated efficacy both forms bLf at 1 mg/mL infection Vero cells by SARS-CoV-2. As assessed...
Abstract Background Host factors that influence Congenital Zika Syndrome (CZS) outcome remain elusive. Interferons have been reported as the main antiviral factor in and other flavivirus infections. Methods We accessed samples from pregnancies, conducted a case-control study to verify whether interferon alfa receptor 1 ( IFNAR1 ) lambda 2 4 IFNL2/4 single nucleotide polymorphisms (SNPs) contribute CZS newborn we characterized placenta gene expression profile at term. Findings Newborns...
Sustained virologic response (SVR) in chronic hepatitis C (CHC) treatment denotes that the host genetics controls immune and unequivocally contribute to viral clearance or disease severity. In this context, single nucleotide polymorphisms (SNPs) locus of interferon lambda 3 4 genes (
The cellular immune response plays an important role in COVID-19, caused by SARS-CoV-2. This feature makes use of vitro models' useful tools to evaluate vaccines and biopharmaceutical effects. Here, we developed a two-step model the after SARS-CoV-2 infection-induced or spike protein stimulation peripheral blood mononuclear cells (PBMC) from both unexposed COVID-19 (primo-infected) individuals (Step1). Moreover, supernatants these cultures were used its effects on lung cell lines (A549)...
The aim of this study was to compare the PSPT standardized in-house as an alternative MPT for potency assays pertussis component. Statistical analyses have showed similar values when compared MPT. Significant correlation between results obtained by in vivo and vitro also been observed. Results demonstrated reproducibility accuracy control approach has considered promising use at least during steps production.
Objectives: The aim of this study is to evaluate some mechanisms the immune response people infected with SARS-CoV-2 in both acute infection and early late convalescence phases. Methods: This a cohort 70 cases COVID-19, confirmed by RT-PCR, followed up 60 days. Plasma Samples clinical data were. Viral load, blood count, indicators inflammation were parameters evaluated. Cellular was evaluated flow cytometry Luminex immunoassays. Results: In severe group, hypertension only reported...
Assess the level of measles vaccine-induced neutralizing antibodies against D8 genotype and persistence humoral cell-mediated immunity in children who received their first dose measles, mumps, rubella vaccine eight years previously.