A5062963511- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Vaccine Coverage and Hesitancy
- Malaria Research and Control
- Virology and Viral Diseases
- Zoonotic diseases and public health
- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Hepatitis B Virus Studies
- Biotechnology and Related Fields
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Innovation and Socioeconomic Development
- Vector-borne infectious diseases
- Protein purification and stability
- Rural Development and Agriculture
- CRISPR and Genetic Engineering
Fundação Oswaldo Cruz
2015-2025
Ministério da Saúde
2018
Universidade do Estado do Pará
2017
Instituto de Tecnologia e Pesquisa
2009
Administración Nacional de Laboratorios e Institutos de Salud
2003
Universidade Federal da Bahia
1997
The live attenuated 17DD Yellow Fever vaccine is one of the most successful prophylactic interventions for controlling disease expansion ever designed and utilized in larger scale. However, increase on worldwide demands manufacturing restrictions urge more detailed dose sparing studies. establishment complementary biomarkers addition to PRNT Viremia could support a secure decision-making regarding use YF subdoses. present work aimed at comparing serum chemokine cytokine kinetics triggered by...
Background. The live attenuated yellow fever (YF) vaccines have been available for decades and are considered highly effective one of the safest worldwide. Methods. impact YF-17DD-antigens recall on cytokine profiles YF-17DD-vaccinated children were characterized using short-term cultures whole blood samples single-cell flow cytometry. This study enrolled seroconverters nonseroconverters after primovaccination (PV-PRNT+ PV-PRNT−), revaccination (RV-PRNT+), unvaccinated volunteers (UV-PRNT−)....
A single vaccination of Yellow Fever vaccines is believed to confer life-long protection. In this study, results vaccinees who received a dose 17DD-YF immunization followed over 10 y challenge premise. YF-neutralizing antibodies, subsets memory T and B cells as well cytokine-producing lymphocytes were evaluated in groups adults before (NVday0) after (PVday30-45, PVyear1-4, PVyear5-9, PVyear10-11, PVyear12-13) primary vaccination. antibodies decrease significantly from PVyear1-4 PVyear12-13...
OBJECTIVE: To compare the immunogenicity of three yellow fever vaccines from WHO-17D and Brazilian 17DD substrains (different seed-lots). METHODS: An equivalence trial was carried out involving 1,087 adults in Rio de Janeiro. Vaccines produced by Bio-Manguinhos, Fiocruz (Rio Janeiro, Brazil) were administered following standardized procedures adapted to allow blocked randomized allocation participants coded vaccine types (double-blind). Neutralizing antibody titters compared pre-...
ABSTRACT Yellow fever (YF) vaccines (17D-204 and 17DD) are well tolerated cause very low rates of severe adverse events (YEL-SAE), such as serious allergic reactions, neurotropic diseases (YEL-AND), viscerotropic (YEL-AVD). Viral host factors have been postulated to explain the basis YEL-SAE. However, mechanisms underlying occurrence YEL-SAE remain unknown. The present report provides a detailed immunological analysis 23-year-old female patient. patient developed suspected case YEL-AVD with...
Attenuated yellow fever (YF) virus 17D/17DD vaccines are the only available protection from YF infection, which remains a significant source of morbidity and mortality in tropical areas world. The attenuated vaccine, is used worldwide, generates both long-lasting neutralizing antibodies strong T-cell responses. However, on rare occasions, this vaccine has toxic side effects that can be fatal. This study presents design two non-viral DNA-based antigen formulations characterization their...
ABSTRACT All currently licensed yellow fever (YF) vaccines are propagated in chicken embryos. Recent studies of chick cell-derived measles and mumps show evidence two types retrovirus particles, the endogenous avian (EAV) leukosis virus (ALV-E), which originate from embryonic fibroblast substrates. In this study, we investigated substrate-derived contamination YF produced by three manufacturers (YF-vax [Connaught Laboratories], Stamaril [Aventis], YF-FIOCRUZ [FIOCRUZ-Bio-Manguinhos])....
The smallpox worldwide eradication was the major world public health achievement. binomial - vaccines and immunization continues to demonstrate very high performance in prevention control of other diseases preventable by vaccination. new global initiatives on vaccination, such as GAVI, have made possible introduction important preventing million children deaths poorest countries world. National Immunization Program Brazil is also being strengthened, with several into basic calendar...
OBJECTIVE: To compare the reactogenicity of three yellow fever (YF) vaccines from WHO-17D and Brazilian 17DD substrains (different seed-lots) placebo. METHODS: The study involved 1,087 adults eligible for YF vaccine in Rio de Janeiro, Brazil. Vaccines produced by Bio-Manguinhos, Fiocruz (Rio Brazil) were administered ("day 0") following standardized procedures adapted to allow blinding blocked randomization participants coded types. Adverse events after immunization ascertained an interview...
Age-related seroprevalence studies that have been conducted in Brazil indicated a transition from high to medium endemicity of hepatitis A virus (HAV) infection the population. However, most these focused on urban populations experience lower incidence rates HAV infection. In current study, prevalence anti-HAV antibodies was investigated children with low socioeconomic status (SES) live periphery three capital cities Brazil. total 1,162 dried blood spot samples were collected individuals...
The Yellow Fever (YF) vaccination is recommended for people living in endemic areas and represents the most effective strategy to reduce risk of infection. Previous studies have warned that booster regimens should be considered guarantee long-term persistence 17DD-YF-specific memory components adults with YF-virus circulation. Considering lower seroconversion rates observed children (9-12 month age) as compared adults, this study was designed order access duration immunity single-dose...
Yellow fever (YF) is an acute infectious disease caused by the yellow virus which transmitted mosquitoes. Neotropical primates are susceptible to infection, often presented as epizootic outbreaks. The aim was evaluate and characterize immune response against YF in different species of neotropical from Belo Horizonte Zoo. Vaccine 17DD administered 24 primates, with a single subcutaneous dose. Clinical exams, RNAemia, detection IgG neutralizing antibodies YFV were performed. In addition,...
Summary This work studied the replication sites of hepatitis A virus (HAV) in cynomolgus monkeys ( Macaca fascicularis ) after intravenous inoculation. The were inoculated with Brazilian strain (HAF‐203). Monkeys euthanized on days 15, 30, 45 and 60 postinoculation (pi). Liver samples, submandibular salivary gland, mesenteric lymph node tonsils removed for virological pathological evaluation. Immunofluorescence analyses liver gland sections using confocal laser scanning microscopy revealed...
Background This study aimed to compare the cytokine-mediated immune response in children submitted primary vaccination with YF-17D-213/77 or YF-17DD yellow fever (YF) substrains. Methods A non-probabilistic sample of eighty healthy vaccinated (PV) was selected on basis their previously known humoral YF vaccines. The were categorized according YF-neutralizing antibody titers (PRNT) and referred as seroconverters (PV-PRNT+) nonseroconverters (PV-PRNT−). Following revaccination YF-17DD,...
Vaccination is the most important measure for prevention and control of yellow fever. It recommended by World Health Organization (WHO) residents endemic areas travelers to risk areas. In 2013, WHO discontinued recommendation booster doses every 10 years, indicating a single dose as sufficient lifelong protection.Considering lower immune response YF vaccine in children compared adults, this study was set out assess duration immunity vaccinated first two years life.This cross-sectional...
Flaviviruses cause severe acute febrile and haemorrhagic infections, including dengue yellow fever the pathogenesis of these infections is caused by an exacerbated immune response. Dendritic cells (DCs) are targets for virus (DENV) (YF) replication first cell population to interact with viruses during a natural infection, which leads induction protective immunity in humans. We studied infectivity DENV2 (strain 16681), YF vaccine (YF17DD) chimeric YF17D/DENV2 monocyte-derived DCs vitro regard...
Introduction: The World Health Organization (WHO) recommends one single dose of the Yellow Fever (YF) vaccine based on studies antibody persistency in healthy adults. We assessed prevalence and titers YF virus neutralizing antibodies previously vaccinated persons aged ≥ 60 years, comparison to younger also evaluated correlation between time since vaccination among participants who received dose, seropositivity prior or within past 10 years. Methods: 18 years were included. determined by...
We describe a case of poliomyelitis in 3-year-old Argentinean boy with X-linked hypogammaglobulinemia. The child had no history polio vaccination, but poliovirus isolated from stool sample 97.2% genetic similarity to the Sabin 1 vaccine strain. According WHO definition, this is first reported vaccine-derived infection (iVDPV) recorded continental Latin America. use live oral (OPV) eradication initiative has led elimination indigenous wild and certification Americas as polio-free September...
During recent years, vaccination against hepatitis A has been implemented in several countries. It is expected that the increase mass will eventually result a decreased prevalence of anti-HAV antibodies general population. For this reason, suitable clinical sample for diagnosis must be sufficiently sensitive to enable detection lower titers. In study, feasibility using dried blood spots (DBS) was assessed after natural infection and vaccination. Seventy-four DBS paired plasma samples were...