A5018625719- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Cytomegalovirus and herpesvirus research
- Long-Term Effects of COVID-19
- Immunotherapy and Immune Responses
- Cardiovascular and exercise physiology
- Exercise and Physiological Responses
- Parasites and Host Interactions
- Autoimmune and Inflammatory Disorders Research
- Malaria Research and Control
- Respiratory viral infections research
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Immune responses and vaccinations
- Vaccine Coverage and Hesitancy
- Vibrio bacteria research studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neurotransmitter Receptor Influence on Behavior
Fundação Oswaldo Cruz
2015-2025
Centro Universitário de Belo Horizonte
2011
Universidade Federal de Minas Gerais
2002-2003
The live attenuated 17DD Yellow Fever vaccine is one of the most successful prophylactic interventions for controlling disease expansion ever designed and utilized in larger scale. However, increase on worldwide demands manufacturing restrictions urge more detailed dose sparing studies. establishment complementary biomarkers addition to PRNT Viremia could support a secure decision-making regarding use YF subdoses. present work aimed at comparing serum chemokine cytokine kinetics triggered by...
Background. The live attenuated yellow fever (YF) vaccines have been available for decades and are considered highly effective one of the safest worldwide. Methods. impact YF-17DD-antigens recall on cytokine profiles YF-17DD-vaccinated children were characterized using short-term cultures whole blood samples single-cell flow cytometry. This study enrolled seroconverters nonseroconverters after primovaccination (PV-PRNT+ PV-PRNT−), revaccination (RV-PRNT+), unvaccinated volunteers (UV-PRNT−)....
Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about immunopathogenesis.To define the immunologic biomarkers that correlate acute ZIKV infection.We characterized levels circulating cytokines, chemokines, growth factors 54 infected patients both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison 100 age-matched controls was...
A single vaccination of Yellow Fever vaccines is believed to confer life-long protection. In this study, results vaccinees who received a dose 17DD-YF immunization followed over 10 y challenge premise. YF-neutralizing antibodies, subsets memory T and B cells as well cytokine-producing lymphocytes were evaluated in groups adults before (NVday0) after (PVday30-45, PVyear1-4, PVyear5-9, PVyear10-11, PVyear12-13) primary vaccination. antibodies decrease significantly from PVyear1-4 PVyear12-13...
Massive vaccination positively impacted the SARS-CoV-2 pandemic, being a strategy to increase titers of neutralizing antibodies (NAbs) in population. Assessing NAb levels and understanding kinetics responses is critical for evaluating immune protection. In this study, we optimized validated PRNT50 assay assess 50% virus neutralization evaluated its accuracy measure NAbs original strain or variant SARS-CoV-2. The optimal settings were selected, such as cell (2 × 105 cells/well) CMC (1.5%)...
Summary We describe how hookworms interact with their human hosts by comparing lymphocyte phenotyping, proliferative responses, and cytokine chemokine secretion patterns in adults who are either mono‐infected Necator americanus or egg‐negative controls resident an area of high transmission Brazil. Cellular immune responses against crude hookworm antigen extracts from different developmental stages were evaluated simultaneously. Principal component analysis (PCA) was used to reduce the...
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed evaluate cytokine profile IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at 81 IE patients, compared 34 healthy subjects 30 patients non-IE infections, matched age gender. Mean was 47±17 years (range,...
Differential serological diagnosis of Chagas disease and leishmaniasis is difficult owing to cross-reactivity resulting from the fact that parasites cause these pathologies share antigenic epitopes. Even with optimized assays use parasite-specific recombinant antigens, inconclusive test results continue be a problem. Therefore, new tests high sensitivity specificity are needed. In present work, we developed evaluated performance flow cytometric method, referred as FC-TRIPLEX Chagas/Leish...
Zika virus is an emerging arbovirus of the family Flaviviridae and genus Flavivirus that until 2007 was restricted to a few cases mild illness in Africa Asia.We report case atrial fibrillation disclosed during acute infection 49-year-old man. Different biological samples were analyzed for molecular diagnosis by real-time PCR, however only saliva specimen positive. The patient's wife tested positive serum sample, although she asymptomatic carrier. Moreover, complete overview biomarkers,...
Background Effective and safe vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are critical to controlling the COVID-19 pandemic will remain most important tool in limiting spread of virus long after is over. Methods We bring pioneering contributions on maintenance immune response over a year real-life basis study 1,587 individuals (18-90 yrs, median 39 yrs; 1,208 female/379 male) who underwent vaccination with two doses CoronaVac BNT162b2 booster 6-months...
Aging is associated with several physiological changes, including a remarkable remodeling of the immune system. Herein, rhythms and shifts in serum mediators were characterized decade lifespan as longitudinal community-based prospective investigation from Bambuí Health Study. The study population included paired samples 713 subjects survivors original BHAS cohort at 10-years Follow-up, categorized into 5-years age range intervals (60-64Yrs towards 90 + Yrs). Quantification soluble carried...
In 2013, the World Health Organization (WHO) withdrew recommendation of booster doses yellow fever (YF) vaccine, based on retrospective and cross-sectional studies that showed lifelong protective immunity from a single dose. Currently, transmission in Brazil occurs only through jungle (sylvatic) cycle. However, high vector density Aedes aegypti , which transmits other orthoflaviviruses, is concern for expansion YF regions country. We conducted cohort study to assess duration vaccine-induced...
Yellow Fever (YF) vaccination is suggested to induce a large number of adverse events (AE) and suboptimal responses in patients with autoimmune diseases (AID); however, there have been no studies on 17DD-YF primary performance AID. This prospective non-interventional study conducted between March July, 2017 assessed the safety immunogenicity planned Adult AID (both sexes) were enrolled, along healthy controls (HC), at single hospital (Vitória, Brazil). Included referred for by...
The Yellow Fever (YF) vaccination is recommended for people living in endemic areas and represents the most effective strategy to reduce risk of infection. Previous studies have warned that booster regimens should be considered guarantee long-term persistence 17DD-YF-specific memory components adults with YF-virus circulation. Considering lower seroconversion rates observed children (9-12 month age) as compared adults, this study was designed order access duration immunity single-dose...
Background This study aimed to compare the cytokine-mediated immune response in children submitted primary vaccination with YF-17D-213/77 or YF-17DD yellow fever (YF) substrains. Methods A non-probabilistic sample of eighty healthy vaccinated (PV) was selected on basis their previously known humoral YF vaccines. The were categorized according YF-neutralizing antibody titers (PRNT) and referred as seroconverters (PV-PRNT+) nonseroconverters (PV-PRNT−). Following revaccination YF-17DD,...
Vaccination is the most important measure for prevention and control of yellow fever. It recommended by World Health Organization (WHO) residents endemic areas travelers to risk areas. In 2013, WHO discontinued recommendation booster doses every 10 years, indicating a single dose as sufficient lifelong protection.Considering lower immune response YF vaccine in children compared adults, this study was set out assess duration immunity vaccinated first two years life.This cross-sectional...