A5090487428- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- SARS-CoV-2 detection and testing
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- COVID-19 epidemiological studies
- Liver Disease Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- Vaccine Coverage and Hesitancy
- Mosquito-borne diseases and control
- COVID-19 Impact on Reproduction
- COVID-19 and healthcare impacts
- Parvovirus B19 Infection Studies
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Biochemical and Molecular Research
- Influenza Virus Research Studies
- Venomous Animal Envenomation and Studies
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Animal Virus Infections Studies
- Retinal and Optic Conditions
- Plant Virus Research Studies
Faculdade de Medicina de São José do Rio Preto
2019-2025
Universidade Estadual Paulista (Unesp)
2017-2022
University of Leeds
2019-2020
Institute of Structural and Molecular Biology
2020
The emergence of the Brazilian variant concern, Gamma lineage (P.1), impacted epidemiological profile COVID-19 cases due to its higher transmissibility rate and immune evasion ability.
Zika virus (ZIKV) has been associated with serious health conditions, and an intense search to discover different ways prevent treat ZIKV infection is underway. Berberine emodin possess several pharmacological properties have shown be particularly effective against the entry replication of viruses. We show that berberine trigger a virucidal effect on ZIKV. When was exposed 160 µM berberine, reduction 77.6% in infectivity observed; when used (40 µM), this approximately 83.3%. Dynamic light...
Abstract Background Cellular entry of SARS-CoV-2 has been shown to rely on angiotensin-converting enzyme 2 (ACE2) receptors, whose expression in the testis is among highest body. Additionally, risk mortality seems higher male COVID-19 patients, and though much published since first cases COVID-19, there remain unanswered questions regarding impact testes potential consequences for reproductive health. We investigated testicular alterations non-vaccinated deceased COVID-19-patients, precise...
Hepatitis C virus (HCV) is one of the main causes liver disease and transplantation worldwide. Current therapy expensive, presents additional side effects viral resistance has been described. Therefore, studies for developing more efficient antivirals against HCV are needed. Compounds isolated from animal venoms have shown antiviral activity some viruses such as Dengue virus, Yellow fever Measles virus. In this study, we evaluated effect complex crotoxin (CX) its subunits crotapotin (CP)...
The rapid development of efficacious and safe vaccines against coronavirus disease 2019 (COVID-19) has been instrumental in mitigating the transmission severe acute respiratory syndrome 2 (SARS-CoV-2). Moreover, emergence SARS-CoV-2 variants raised concerns on efficacy these vaccines. Herein, we report two cases breakthrough infections with P1 variant patients vaccinated CoronaVac, which is one authorized for emergency use Brazilian immunization program. Our observations suggest that vaccine...
The emergence of the new SARS-CoV-2 Omicron variant, which is known to have a large number mutations when compared other variants, brought light concern about vaccine escape, especially from neutralization by antibodies induced vaccination.Based on viral microneutralization assays, we evaluated in 90 individuals impact antibody induction, against booster dose BNT162b2 mRNA after CoronaVac primary vaccination scheme.Here show that percentage seroconverted 30 and 60 days scheme was 16.6% 10%,...
Background Effective and safe vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are critical to controlling the COVID-19 pandemic will remain most important tool in limiting spread of virus long after is over. Methods We bring pioneering contributions on maintenance immune response over a year real-life basis study 1,587 individuals (18-90 yrs, median 39 yrs; 1,208 female/379 male) who underwent vaccination with two doses CoronaVac BNT162b2 booster 6-months...
Enteric viruses, such as the Aichi virus (AiV), pose a potential health risk due to their high excretion rates through fecal elimination, limited removal during treatment processes, and prolonged survival, highlighting need assess for exposure disease transmission sanitation systems. This study investigated prevalence of AiV at three key stages sewage in city São José do Rio Preto, Paulo state, Brazil, well its viral concentrations, infectious potential, molecular characterization. The data...
Hepatitis C virus (HCV) affects about 170 million people worldwide. The current treatment has a high cost and variable response rates according to the genotype. Acridones, group of compounds extracted from natural sources, showed potential antiviral actions against HCV. Thus, this study aimed evaluate effect panel 14 synthetic acridones on HCV life cycle. were screened using an Huh7.5 cell line stably harbouring genotype 2a subgenomic replicon SGR-Feo-JFH-1. Cells incubated in presence or...
Abstract High frequency screening of populations has been proposed as a strategy in facilitating control the COVID-19 pandemic. We use computational modeling, coupled with clinical data from rapid antigen tests, to predict impact frequent viral testing on spread and outcomes. Using patient nasal or nasopharyngeal swab specimens, we demonstrate that sensitivity/specificity two tests compared quantitative real-time polymerase chain reaction (qRT-PCR) are 82.0%/100% 84.7%/85.7%, respectively;...
ABSTRACT Although much has been published since the first cases of COVID-19, there remain unanswered questions regarding SARS-CoV-2 impact on testes and potential consequences for reproductive health. We investigated testicular alterations in deceased COVID-19-patients, precise location virus, its replicative activity, molecules involved pathogenesis. found that tropism is higher than previously thought reliable viral detection testis requires sensitive nanosensoring or RT-qPCR using a...
Abstract In recent years, synthetic peptides have been considered promising targets for drug development that possess low side-effects, are cost-effective and susceptible to rational design. Hecate was initially described as a potent bacterial inhibitor subsequently an anticancer with functions related its lipid interaction property. Viruses, such hepatitis C virus (HCV), lipid-dependent life cycle could be affected by in many ways. Here, we assessed modifications on Hecate’s N-terminus...
Abstract High frequency screening of populations has been proposed as a strategy in facilitating control the COVID-19 pandemic. We use computational modeling, coupled with clinical data from rapid antigen tests, to predict impact frequent viral testing on spread and outcomes. Using patient nasal or nasopharyngeal swab specimens, we demonstrate that sensitivity/specificity two tests compared quantitative real-time polymerase chain reaction (qRT-PCR) are 80.0%/91.1% 84.7%/85.7%, respectively;...
The emergence of several SARS-CoV-2 lineages presenting adaptive mutations is a matter concern worldwide due to their potential ability increase transmission and/or evade the immune response. While performing epidemiological and genomic surveillance in samples from Porto Ferreira-São Paulo-Brazil, we identified sequences classified by pangolin as B.1.1.28 harboring Spike L452R mutation, RBD region. Phylogenetic analysis revealed that these grouped into monophyletic branch, with others...
Abstract Introduction: Vaccines are essential for the prevention and control of several diseases, indeed, monitoring immune response generated by vaccines is crucial. The vaccination against SARS-CoV-2 in children adolescents not well defined regarding to intensity medium long-term duration a protective response, which may point out need booster doses might support decisions public health. Objective study aims evaluate immunogenicity safety inactivated vaccine (CoronaVac) two-dose primary...
Hepatitis C virus (HCV) is an important human pathogen causing 400 000 chronic liver disease-related deaths annually. Until recently, the majority of laboratory-based investigations into biology HCV have focused on genotype 2 isolate, JFH-1, involving replicons and infectious cell culture systems. However, one eight major genotypes there great sequence variation among these (>30 % nucleotide divergence). In this regard, 3 second most common accounts for 30 global cases. Further, associated...
Hepatitis C virus (HCV) genotype 3 presents a high level of both baseline and acquired resistance to direct-acting antivirals (DAAs), particularly those targeting the NS5A protein. To understand this we studied cohort Brazilian patients treated with DAA, daclatasvir nucleoside analogue, sofosbuvir. We observed novel substitution at amino acid residue 98 [serine glycine (S98G)] in who relapsed post-treatment. The effect on replication fitness DAAs was evaluated using two subgenomic replicons....
Abstract The emergence of the new Brazilian variant concern, P.1 lineage (Gamma), raised concern about its impact on epidemiological profile COVID-19 cases due to higher transmissibility rate and immune evasion ability. Using 272 whole-genome sequences combined with data, we showed that introduction in São José do Rio Preto, Paulo, Brazil, was followed by displacement eight circulating SARS-CoV-2 variants a rapid increase prevalence two months after first detection. Our findings support is...
The emergence of the new SARS-CoV-2 Omicron variant, which is known to accumulate a huge number mutations when compared other variants, brought light concern about vaccine escape, especially from neutralization by antibodies induced vaccination. In this scenario, we evaluated impact on antibody induction, against booster dose BNT162b2 mRNA after CoronaVac primary vaccination scheme. percentage seroconverted individuals 30 and 60 days scheme was 17% 10%, respectively. After administration,...