A5073952230- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Malaria Research and Control
- Hemoglobinopathies and Related Disorders
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Inflammasome and immune disorders
- Blood groups and transfusion
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Virology and Viral Diseases
- Neonatal Health and Biochemistry
- Leprosy Research and Treatment
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Complement system in diseases
- Immunodeficiency and Autoimmune Disorders
- Parasites and Host Interactions
- Iron Metabolism and Disorders
- Eosinophilic Disorders and Syndromes
- Autoimmune and Inflammatory Disorders Research
- Hepatitis B Virus Studies
Universidade Nilton Lins
2015-2024
Fundação de Medicina Tropical
2014-2023
Universidade Federal do Amazonas
2014-2023
Universidade do Estado do Amazonas
2014-2023
Amazon (Germany)
2021
Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas
2019-2020
Complexo Hospitalar Universitário Professor Edgard Santos
2008-2019
Universidade Federal da Bahia
2008-2019
Inserm
1992-2018
Assistance Publique – Hôpitaux de Paris
2002-2018
The pathogenesis of Chagas disease cardiomyopathy (CCC) is not well understood. Since studies show that myocarditis more frequent during the advanced stages disease, and prognosis CCC worse than other dilated cardiomyopathies non-inflammatory aetiology, which suggest inflammatory infiltrate plays a major role in myocardial damage. In last decade, increasing evidence has shown cytokines chemokines play generation tissue patients have an increased peripheral production Th1 IFN-gamma TNF-alpha...
Malaria is one of the most significant infectious diseases in world and responsible for a large proportion infant deaths. Toll-like receptors (TLRs), key components innate immunity, are central to countering infection. Variants TLR-signaling pathway associated with susceptibility diseases.We genotyped single nucleotide polymorphisms (SNPs) genes patients mild malaria individuals asymptomatic Plasmodium infections by means polymerase chain reaction.Genotype distributions TLR-1 I602S differed...
Oropouche fever is the second most frequent arboviral infection in Brazil, surpassed only by dengue. virus (OROV) causes large and explosive outbreaks of acute febrile illness cities villages Amazon Central-Plateau regions. Cerebrospinal fluid (CSF) samples from 110 meningoencephalitis patients were analyzed. The RNA extracted was submitted to reverse transcription-polymerase chain reaction sequencing identify OROV. Three CSF showed presence OROV causing central nervous system (CNS). These...
Acute infections of the central nervous system (CNS) can be caused by various pathogens. In this study, presence herpesviruses (HHV), enteroviruses (EVs), and arboviruses were investigated in CSF samples from 165 patients with suspected CNS viral infection through polymerase chain reaction (PCR) reverse transcriptase PCR. The genomes one or more agents detected 29.7% (49/165) samples. EVs predominant (16/49; 32.6%) followed Epstein-Barr virus (EBV) (22.4%), Varicella-Zoster (VZV) (20.4%),...
Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about immunopathogenesis.To define the immunologic biomarkers that correlate acute ZIKV infection.We characterized levels circulating cytokines, chemokines, growth factors 54 infected patients both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison 100 age-matched controls was...
N-Acetylglucosamine (GlcNAc) is the major immunoepitope of group A streptococcal cell wall carbohydrates. Antistreptococcal antibodies cross-reactive with anti-GlcNAc and laminin are present in sera patients rheumatic fever. The cross-reactivity these human heart valvular endothelium underlying basement membrane has been suggested to be a possible cause immune-mediated valve lesion. Mannose-binding lectin (MBL) encoded by MBL2 gene, soluble pathogen recognition receptor, high affinity for...
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi. Among T. cruzi-infected individuals, only a subgroup develops severe chronic cardiomyopathy (CCC); majority remain asymptomatic. cruzi displays numerous ligands for Toll-like receptors (TLRs), which are an important component of innate immunity that lead to transcription proinflammatory cytokines nuclear factor-kappaB. Because play role in CCC, we hypothesized single-nucleotide polymorphisms (SNPs) genes encode proteins...
CYP2D6 pathway mediates the activation of primaquine into active metabolite(s) in hepatocytes. is highly polymorphic, encoding isoforms with normal, reduced, null or increased activity. It hypothesized that Plasmodium vivax malaria patients defective function would be at risk for failure to prevent recurrence. The aim this study was investigate association polymorphisms and inferred phenotypes recurrence from Western Brazilian Amazon, following chloroquine/primaquine combined therapy....
Cutaneous leishmaniasis (CL) is characterized by high production of pro-inflammatory cytokines and development pathology. Individuals with subclinical L. braziliensis infection (SC) have a positive skin test to leishmania, but do not develop disease. We evaluated whether the downregulation inflammatory response in SC mediated IL-10 IL-27 IL-17 associated control infection. Participants include individuals, patients CL healthy subjects. Cytokines protein mRNA were detected ELISA real-time...
Dengue is a vector-borne disease in the tropical and subtropical region of world transmitted by mosquito Aedes aegypti. In state Amazonas, Brazil during 2011 outbreak dengue all four virus (DENV) serotypes circulating simultaneously were observed. The aim study was to describe clinical epidemiology Manaus, capital city where DENV co-circulating simultaneously.Patients with acute febrile illness dengue, enrolled at Fundação de Medicina Tropical Dr. Heitor Viera Dourado (FMT-HVD), referral...
The resistance of Plasmodium vivax to chloroquine has become an obstacle control strategies based on the use anti-malarials. current study investigated association between P. CQ-resistance in vivo with copy number variation and mutations promoter region pvcrt-o pvmdr1 genes. included patients that received supervised treatment primaquine. Recurrences were actively recorded during this period. Among 60 vivax, 25 CQ-resistant 35 CQ-susceptible. A frequency 7.1% multi-copy was observed...
Only a subset of individuals infected with Trypanosoma cruzi develop chronic Chagas cardiomyopathy (CCC). Familial aggregation CCC in areas endemicity indicates that susceptibility may be genetic, which plausible explanation for why only one-third T. cruzi-infected CCC. The monocyte chemoattractant protein-1 (CCL2/MCP-1) has been shown to enhance the uptake murine macrophages and up-regulate inducible nitric oxide synthase/nitric system, consequent increased production controls replication...
INTRODUCTION: Manaus, the capital city of state Amazon with nearly 2 million inhabitants, is located in middle rain forest and has suffered dengue outbreaks since 1998. METHODS: In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying virus serotypes. RESULTS: Acute phase sera from 432 patients tested for presence virus. Out patients, 137 (31.3%) found to be positive. All four serotypes observed. CONCLUSIONS: The...
Zika virus is an emerging arbovirus of the family Flaviviridae and genus Flavivirus that until 2007 was restricted to a few cases mild illness in Africa Asia.We report case atrial fibrillation disclosed during acute infection 49-year-old man. Different biological samples were analyzed for molecular diagnosis by real-time PCR, however only saliva specimen positive. The patient's wife tested positive serum sample, although she asymptomatic carrier. Moreover, complete overview biomarkers,...
Introduction The clinical outcome to Leishmania-infection is determined by the individual adaptive immune T helper cell responses and their interactions with parasitized host cells. An early development of a proinflammatory response (Th1 response) necessary for resolution. Toll-interacting protein (TOLLIP) regulates human Toll-like receptors signaling pathways down regulating cytokines interleukin-6 (IL-6) tumor necrosis factor alpha (TNF-α) inducing ant-inflammatory cytokine interleukin-10...
Immunogenetic host factors are associated with susceptibility or protection to tuberculosis (TB). Strong associations of HLA class II genes TB reported. We analyzed the HLA-DRB1*04 alleles identify subtypes pulmonary and their interaction risk such as alcohol, smoking, gender in 316 patients 306 healthy individuals from Brazilian Amazon. The was prevalent (p<0.0001; OR = 2.94; 95% CI 2.12 4.08). Direct nucleotide sequencing DRB1 exon 2 identified nine HLA-DRB1*04. subtype HLA-DRB1*04:11:01...
It is not understood why only a subset of individuals infected with Trypanosoma cruzi develop chronic Chagas cardiomyopathy (CCC). Patients CCC display high levels circulating proinflammatory cytokines. Heart-infiltrating lymphocytes from patients also express cytokines (tumor necrosis factor- alpha and interferon- gamma ) that are detectable in biopsy samples surgical heart-tissue samples. BAT1, putative anti-inflammatory gene, presents functional polymorphisms its promoter region influence...
ABSTRACT American tegumentary leishmaniasis (ATL) (also known as cutaneous [CL]) is caused by various species of protozoa the genus Leishmania . The diagnosis achieved on a clinical, epidemiological, and pathological basis, supported positive parasitological exams demonstration leishmanin delayed-type hypersensitivity. Serological assays are not routinely used in because many considered to have low sensitivity particular causing disease can lead variable performance. In present study, we...
Chagas disease, caused by Trypanosoma cruzi infection, displays clinical heterogeneity and may be attributable to differential genetic susceptibility. Chronic cardiomyopathy (CCC) develops only in a subset of T. cruzi-infected individuals lead heart failure that has worse course leads reduced life expectancy, compared with other etiologies. Proinflammatory cytokines play key role the development CCC. Clinical, genetic, epidemiological studies have linked lymphotoxin-alpha (LTA),...