A5064597342- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Leptospirosis research and findings
- Eosinophilic Disorders and Syndromes
- Phytochemistry Medicinal Plant Applications
- Parasites and Host Interactions
- Clinical Laboratory Practices and Quality Control
- Hepatitis Viruses Studies and Epidemiology
- History of Medicine and Tropical Health
- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- Pesticide Exposure and Toxicity
- Diverticular Disease and Complications
- Gastrointestinal disorders and treatments
- Chemistry Education and Research
- Heat shock proteins research
- Viral gastroenteritis research and epidemiology
- Toxin Mechanisms and Immunotoxins
- Cystic Fibrosis Research Advances
- Protein purification and stability
- Hemophilia Treatment and Research
- Evasion and Academic Success Factors
- Mosquito-borne diseases and control
Instituto de Medicina Tropical
1988-2025
Universidade de São Paulo
2009-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2023-2024
Instituto de Medicina Avançada
1984-2016
Universidade Brasil
1988
Areas where mucocutaneous leishmaniasis, kala-azar and Chagas' disease are prevalent often overlap in Latin America. Due to a variable degree of cross-reactivity between the three etiological agents antibodies synthesized during course disease, test able distinguish them is greatly needed. Saline-extracted antigen from live Trypanosoma cruzi epimastigotes behaved towards sera immunoenzymatic assays (ELISA) with same specificity sensitivity as used routinely for such tests. In tests using...
Routine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of Leishmania infantum rHsp83 compared with L. major-like total promastigote antigen diagnosis cutaneous (CL), mucosal (ML), and leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA-L. when considering either CL/ML (P = 0.041) or all leishmaniasis patients 0.013). When...
ABSTRACT American tegumentary leishmaniasis (ATL) (also known as cutaneous [CL]) is caused by various species of protozoa the genus Leishmania . The diagnosis achieved on a clinical, epidemiological, and pathological basis, supported positive parasitological exams demonstration leishmanin delayed-type hypersensitivity. Serological assays are not routinely used in because many considered to have low sensitivity particular causing disease can lead variable performance. In present study, we...
Background The development of rK39-based immunochromatographic rapid diagnostic tests represents an important advance for serodiagnosis visceral leishmaniasis, being cheap and easy to use at the point care (POC). Although rK39 have considerably improved sensitivity specificity serological compared with total antigens, great variability in was reported. This study aimed evaluation "Kalazar Detect™ Rapid Test, Whole Blood" (Kalazar Detect RDT) Visceral Leishmaniasis (VL) diagnosis using oral...
Abstract Background Immunosuppression increases the risk of primary cutaneous leishmaniasis (CL) infection and its reactivation. This systematic review examined CL reactivation in immunosuppressed patients, including those with autoimmune diseases solid organ transplant recipients. Methods A comprehensive literature search was conducted across multiple databases for studies published between May 1990 2024. Results Nine cases were selected, involving associated immunosuppressive therapy...
Abstract Background Recently, there has been an increase in the incidence of cutaneous leishmaniasis (CL), which represents important health problem. This may be related to epidemiologic expansion infective agent and tourism tropical areas. The difficulty clinical diagnosis, mainly areas CL is not first consideration local physicians, intensified efforts describe diagnostic tests, should specific, sensitive, practical. Amongst new tests described are those including nucleic acid...
The serologic assay is an important tool in the diagnosis of leishmaniasis. One most commonly used tests enzyme-linked immunosorbent (ELISA). Since total Leishmania promastigotes are as antigen routine assay, false-positive reactions frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, that determines less cross-reactivity has been pursued for serodiagnosis In present study we analyzed use recombinant infantum heat shock protein (Hsp) 83 ELISA...
American tegumentary leishmaniasis (ATL) diagnosis is an open question, and the search for a solution urgent. The available tests that detect etiological agent of infection are specific ATL diagnosis. However, they present disadvantages, such as low sensitivity need invasive procedures to obtain samples. Immunological methods (leishmanin skin test anti- Leishmania antibodies) good alternatives ATL. Presently, we face problems with disease confirmation due discontinuity in production...
A dot enzyme-linked immunosorbent assay (dot ELISA) was evaluated and compared with a standard microplate ELISA (immunoglobulin G [IgG] for the serological diagnosis of mucocutaneous leishmaniasis. The two assays were used to test 113 serum specimens from following groups: normal individuals patients deep mycoses, toxoplasmosis, leishmaniasis, visceral Chagas' disease, malaria, schistosomiasis. Both tests exhibited cross-reactivity when testing cases leishmaniasis disease. proved be...
In the Americas, visceral leishmaniasis (VL) is caused by protozoan Leishmania infantum, leading to death if not promptly diagnosed and treated. Brazil, disease reaches all regions, in 2020, 1,933 VL cases were reported with 9.5% lethality. Thus, an accurate diagnosis essential provide appropriate treatment. Serological based mainly on immunochromatographic tests, but their performance may vary location, evaluation of diagnostic alternatives necessary. this study, we aimed evaluate ELISA...
Abstract Background There is little information about the frequency of Leishmania infection in asymptomatic people living with HIV (PLWH) and performance laboratory diagnostic methods coinfected patients Latin America. The main objective this study to evaluate spp. HIV-infected an urban area Brazil. Methods To detect infection, tests were performed anti- antibodies (ELISA using Leptomonas seymouri antigens; ELISA rK39 rK28 indirect fluorescent-antibody test (IFAT); direct agglutination...
The performance of an antigen L. major-like promastigotes for the serological diagnosis mucocutaneous leishmaniasis in IgG-immunofluorescent test was compared to that braziliensis braziliensis. Each used two hundred and twenty-four sera etiologies such as leishmaniasis, deep mycoses, toxoplasmosis, malaria, Chagas' disease, visceral anti-nuclear factor, schistosomaiasis, rheumatoid factor normal controls. Agreement between responses each high: 77.2% leishmaniases agreed on a positive or...
Leishmania braziliensis braziliensis(MHOM/BR/75/M2903) was grown in Schneider's Drosophila medium. In one set of experiments promastigotes were already adapted to the medium by means serial passages whereas second cells a biphasic and transfered liquid. Growth more abundant for culture cells; degenerate small numbers as well dead ones present from day 5 liquid 3 newly cells. Synthesis surface antigens differed according length cell assessed titer five mucocutaneous leishmaniasis sera on...
Visceral leishmaniasis is common in Brazil and caused by Leishmania (Leishmania) infantum/chagasi. Post-kala-azar dermal frequently follows visceral L. donovani, para-kala-azar refers to an uncommon presentation wherein it occurs simultaneously along with leishmaniasis. While post-kala-azar only occasionally infantum/chagasi infections, patients concomitant immunosuppression (HIV co-infection). Here, we describe the first case of Brazil. It important raise awareness post- infantum endemic...
The immunosuppressive effect of methotrexate has rarely been associated with reactivation cutaneous leishmaniasis. Here we present a case leishmaniasis patient atypical clinical symptoms without splenomegaly but manifestations after treatment rheumatoid arthritis and blood recovery the parasite. Next-generation sequencing was used to identify Leishmania infantum chagasi in patient's sample.
A 31-year-old male patient developed an ulcer on the glans penis that evolved for three months without healing. We diagnosed it as leishmaniasis using polymerase chain reaction. No immunosuppression or associated diseases were observed. The was treated with meglumine antimoniate cured lesion in a month post-treatment. Here, we report this case of cutaneous at unusual location immunocompetent individual. likely due to bite vector, highlighting need considering among differential diagnosis...
The Authors describe an anti-Leishmania IgA-ELISA assay in mucocutaneous leishmaniasis. Increased titers were found leishmaniasis patients, mainly the first and second year of infection deep mycoses patients showing either mucosal involvement or widespread disease.
This paper deals with the analysis of 10 batches L.major-like and L.(V.) braziliensis antigens added or not a proteases inhibitor evaluated by means an IgG-ELISA on three consecutive days using positive standard sera from patients diagnosis American Leishmaniasis previously tested for presence IgG antibodies ELISA. The statistical showed that L. (V.) PMSF-containing antigen did show any difference among testing; without PMSF significance differences two-way ANOVA significant between...