A5087992989- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Peptidase Inhibition and Analysis
- Skin and Cellular Biology Research
- Blood Coagulation and Thrombosis Mechanisms
- Autoimmune Bullous Skin Diseases
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Amyotrophic Lateral Sclerosis Research
- Pesticide Exposure and Toxicity
- Cancer-related gene regulation
- Cholinesterase and Neurodegenerative Diseases
- MicroRNA in disease regulation
- Contact Dermatitis and Allergies
- Supramolecular Self-Assembly in Materials
- Monoclonal and Polyclonal Antibodies Research
- Origins and Evolution of Life
- Wnt/β-catenin signaling in development and cancer
- Plant Reproductive Biology
- Histone Deacetylase Inhibitors Research
- Phytochemical compounds biological activities
- Protease and Inhibitor Mechanisms
- Neurogenetic and Muscular Disorders Research
- Nail Diseases and Treatments
- Hedgehog Signaling Pathway Studies
- Biotin and Related Studies
University of Patras
2014-2025
Aristotle University of Thessaloniki
2018-2024
RMIT University
2006-2010
University of Toronto
2009
Human kallikrein 5 (KLK5) is a member of the human gene family serine proteases. Preliminary results indicate that protein, hK5, may be potential serological marker for breast and ovarian cancer. Other studies implicate hK5 with skin desquamation diseases. To gain further insights on physiological functions, we studied its substrate specificity, regulation activity by various inhibitors, identified candidate substrates. After producing purifying recombinant in yeast, determined k(cat)/K(m)...
Die Mittagspause reicht: Ein elektronischer Chip mit nanostrukturierten Mikroelektroden (NMEs) ermöglicht die Analyse der Expressionsprofile von mikroRNAs in nur 30 min – und das ganz ohne enzymatische Verstärkung oder Sequenzmarkierung! Der detektiert Hybridisierung mikroRNA-Analyten an NME-Oberflächen erzeugt eine starke elektrokatalytische Signalverstärkung durch Verwendung eines ultraempfindlichen Redoxreportersystems (siehe Bild).
A novel liposome/hydrogel soft nanocomposite was explored as a controlled drug delivery system. P2VP-PAA-PnBMA biocompatible, pH-responsive triblock terpolymer used an injectable gelator, entrapping PC/Chol liposomes loaded with calcein hydrophilic model drug. The composite hydrogel formed in vitro through pH-induced sol–gel transition by dialysis against buffer under physiological conditions and at polymer concentration low 1 wt %. Excellent control of the release achieved just adjusting...
Netherton Syndrome (NS) is a rare and severe autosomal recessive skin disease which can be life-threatening in infants. The characterized by extensive desquamation, inflammation, allergic manifestations hair shaft defects. NS caused loss-of-function mutations SPINK5 encoding the LEKTI serine protease inhibitor. deficiency results unopposed activities of kallikrein-related peptidases (KLKs) aberrantly increased proteolysis epidermis. Spink5⁻/⁻ mice recapitulate phenotype, display enhanced...
Human tissue kallikreins (hKs) are a family of fifteen serine proteases. Several lines evidence suggest that hKs participate in proteolytic cascade pathways. kallikrein 5 (hK5) has trypsinlike activity, is able to self-activate, and co-expressed various tissues with other hKs. In this study, we examined the ability hK5 activate By using synthetic heptapeptides encompass activation site each recombinant pro-hKs, demonstrated pro-hK2 pro-hK3. We then showed that, following their activation,...
The analysis of panels nucleic acid biomarkers offers valuable diagnostic and prognostic information for cancer management. A cost-effective, highly sensitive electronic chip would offer an ideal platform clinical biomarker readout have maximal utility if it was (i) multiplexed, enabling on-chip assays multiple biomarkers, (ii) able to perform direct (PCR-free) disease-related genes. Here we report a onto which integrate novel nanostructured microelectrodes with directly detect in...
Human kallikrein-related peptidase 6 (KLK6) was cloned as a putative class II tumor suppressor based on its inactivated expression in metastatic breast cancer. Here, we investigated the mechanism(s) underlying silencing of KLK6 gene cancer and implications for progression. We present evidence that tumor-specific loss is due to hypermethylation specific CpGs located proximal promoter. Methylation-dependent binding methyl CpG-binding protein 2 formation repressive chromatin mediated by...
Zyme/protease M/neurosin/human kallikrein 6 (hK6) is a member of the human family trypsin-like serine proteinases and was originally identified as being down-regulated in metastatic breast ovarian tumors when compared with corresponding primary tumors. Recent evidence suggests that hK6 may serve circulating tumor marker cancers. In addition, it described brain Parkinson's disease Alzheimer's patients, where implicated amyloid precursor protein processing. It thus biomarker for these...
The gene family of human kallikrein-related peptidases (KLKs) encodes proteins with diverse and pleiotropic functions in normal physiology as well disease states. Currently, the most widely known KLK is KLK3 or prostate-specific antigen (PSA) that has applications clinical diagnosis monitoring prostate cancer. encompasses largest contiguous cluster serine proteases humans which not interrupted by non-KLK genes. This exceptional unique characteristic KLKs makes them ideal for evolutionary...
KLK6 is a serine protease highly expressed in the nervous system. In synucleinopathies, including Parkinson disease, levels of inversely correlate with α-synuclein CSF. Recently, we suggested that recombinant mediates degradation extracellular directly and via proteolytic cascade involves unidentified metalloproteinase(s). Here, show naturally secreted can readily cleave fibrils have potential for cell-to-cell propagation "a prion-like mechanism". Importantly, KLK6-deficient primary cortical...
Objective: The human kallikrein 10 (KLK10)/normal epithelial cell-specific-1 (NES1) gene is highly expressed in normal mammary, ovary and prostate cells, but its expression dramatically decreased cancer cell lines. Recently, it has been shown that CpG island hypermethylation of the KLK10 responsible for tumor-specific loss certain breast Method: We examined role breast, ovarian cancers. treated cells with demethylating agent 5-aza-2′-deoxycytidine (dC) monitored changes mRNA by RT-PCR...
Netrin-1 (NTN1) is functionally important for the development of nervous system.Interestingly, few recent studies showed that NTN1 may also promote cancer by increasing survival and resistance lung breast cells to apoptosis. Our purpose was investigate whether its receptor DCC be involved in ovarian cancer. The mRNAs were quantified real-time RTPCR normal (10), benign (8) (17) tissues. ALAS1 TBP housekeeping genes used normalization. found overexpressed 76% specimens (13/17) as compared...
Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease certain types of cancer. Antibodies, either polyclonal or monoclonal, exhibit specificity for distinct members the extended kallikrein family, KLK6, were developed. With exception KLK3/PSA, identification generation aptamers, potential new tools with improved characteristics demanded therapeutic...
// Georgios Pampalakis 1 , Osahon Obasuyi Olga Papadodima 2 Aristotelis Chatziioannou Vassileios Zoumpourlis Georgia Sotiropoulou Department of Pharmacy, University Patras, Rion-Patras 26500 National Hellenic Research Foundation, Athens 11635, Greece Correspondence: Sotiropoulou, email: Keywords : Kallikrein-related peptidase 5 (KLK5), breast cancer, mevalonate pathway, oncogenic signaling Received August 1, 2013 Accepted September Published 3, Abstract (KLK5) displays aberrant expression in...
Kallikrein-related peptidase 5 (KLK5) displays aberrant expression in cancer.Recently, we showed KLK5 reconstitution breast cancer cell lines suppresses malignancy.Present study aims to investigate the functional mediated miRNA network on progression, molecular subtype and survival.28 miRNAs were up-regulated 62 down-regulated upon expression.Extracellular matrix (ECM) molecules cell-adhesion pathways most significant KLK5-induced miRNA-mediated regulatory targets.Validation from The Cancer...