A5051348760- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Inflammasome and immune disorders
- Cholesterol and Lipid Metabolism
- Pneumocystis jirovecii pneumonia detection and treatment
- Plant and Fungal Interactions Research
- RNA Interference and Gene Delivery
- Pharmacological Receptor Mechanisms and Effects
- Lipid Membrane Structure and Behavior
- Animal Virus Infections Studies
- Lipid metabolism and biosynthesis
- Nanoparticles: synthesis and applications
- Nanoplatforms for cancer theranostics
- Drug-Induced Hepatotoxicity and Protection
- Advanced Nanomaterials in Catalysis
- Phagocytosis and Immune Regulation
Fundação Oswaldo Cruz
2018-2025
Viruses are obligate intracellular parasites that make use of the host metabolic machineries to meet their biosynthetic needs. Thus, identifying pathways essential for virus replication may lead potential targets therapeutic intervention. The mechanisms and explored by SARS-CoV-2 support its within cells not fully known. Lipid droplets (LD) organelles with major functions in lipid metabolism, energy homeostasis transport, have multiple roles infections inflammation. Here we described...
An unprecedented global outbreak caused by the monkeypox virus (MPXV) prompted World Health Organization to declare a public health emergency of international concern on July 23, 2022. Therapeutics and vaccines for MPXV are not widely available, necessitating further studies, particularly in drug repurposing area. To this end, standardization vitro infection systems is essential. The most robust studies poxviruses Vaccinia virus, there significant gaps understanding replicative cycle MPXV....
SARS-CoV-2 induces major cellular lipid rearrangements, exploiting the host's metabolic pathways to replicate. Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that control metabolism. SREBP1 is associated with regulation fatty acids, whereas SREBP2 controls cholesterol metabolism, and both isoforms droplet (LD) biogenesis. Here, we evaluated effect SREBP in SARS-CoV-2-infected lung epithelial cell line (Calu-3). We showed infection induced activation...
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted public health and world economy fueled a worldwide race to approve therapeutic prophylactic agents, but so far there are no specific antiviral drugs. Understanding biology of virus is first step in structuring strategies combat it, this context several studies have been conducted with aim understanding replication mechanism SARS-CoV-2 vitro systems. In work, using transmission scanning...
Subgenomic RNAs (sgRNAs) are potential markers of active SARS-CoV-2 replication, serving as templates for the synthesis structural and accessory proteins in infectious viral particles. This study aimed to use RT-qPCR quantify sgRNA negative RNA intermediates, assessing replication virus samples inactivated by β-propiolactone (βPL). Inactivated viruses subjected five blind serial passages (BSs) were amplified using primers target envelope (ENV) nucleoproteins (N1 N2) genomic genes, subgenomic...
Ilhéus Virus (ILHV) was first detected in 1944 Ilhéus, state of Bahia, northeast Brazil. During cellular infection, orthoflaviviruses induce changes related both to the replication process, formation complexes, and structures resulting from damage. Although more detailed data are available literature for other orthoflaviviruses, relationship between ILHV, these structures, its cycle, remains unknown. One main objectives this study is characterize primary ultrastructural green monkey kidney...
The Oropouche virus (OROV) is a segmented negative-sense RNA arbovirus member of the Peribunyaviridae family, associated with recurring epidemics fever in Central and South America. Since its identification 1955, OROV has been responsible for outbreaks both rural urban areas, transmission involving sylvatic cycles. This study focuses on characterization an isolate from human clinical sample collected state Rio de Janeiro, non-endemic region Brazil, highlighting ultrastructural morphological...
Despite extensive efforts, no highly effective antiviral molecule exists for treating moderate and severe COVID-19. Nanotechnology has emerged as a promising approach developing novel drug delivery systems to enhance efficacy. Among these, polymeric nanomicelles improve the solubility, bioavailability, cellular uptake of therapeutic agents. In this study, Pluronic F127-based were developed evaluated their activity against SARS-CoV-2. The nanomicelles, formulated using direct dissolution...
Despite the development of specific therapies against severe acute respiratory coronavirus 2 (SARS-CoV-2), continuous investigation mechanism action clinically approved drugs could provide new information on druggable steps virus–host interaction. For example, chloroquine (CQ)/hydroxychloroquine (HCQ) lacks in vitro activity SARS-CoV-2 TMPRSS2-expressing cells, such as human pneumocyte cell line Calu-3, and likewise, failed to show clinical benefit Solidarity Recovery trials. Another...
In the current COVID-19 pandemic, next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent spread this virus within community. Herein, we report synthesis chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined estimating minimum inhibitory concentration (MIC) minimal bactericidal/fungicidal...
Successful SARS-CoV-2 inactivation allows its safe use in Biosafety Level 2 facilities, and the of whole viral particle helps development analytical methods a more reliable immune response, contributing to improvement vitro vivo assays. In order obtain functional product, we evaluated several protocols observed that 0.03% beta-propiolactone for 24 h was best condition tested, as it promoted above 99.99% no cytopathic effect visualized after five serial passages. Moreover, RT-qPCR...
Despite the rapid mass vaccination against COVID-19, emergence of new SARS-CoV-2 variants concern, such as omicron, is still a great distress, and therapeutic options are needed. Bovine lactoferrin (bLf), multifunctional iron-binding glycoprotein available in unsaturated (apo-bLf) saturated (holo-bLf) forms, has been shown to exert broad-spectrum antiviral activity many viruses. In this study, we evaluated efficacy both forms bLf at 1 mg/mL infection Vero cells by SARS-CoV-2. As assessed...
According to the last 2023 Monkeypox (Mpox) Outbreak Global Map from Centres for Disease Control and Prevention (CDC), more than 100 countries with no Mpox infection report cases. Brazil stands out in this group is second country highest number of cases outbreak.To contribute knowledge virus effects a cellular model, which important diagnosis infections not yet included provider´s differential developing viral inhibition strategies.We describe isolation protocol human clinical sample patient...
Dengue, considered the most important arthropod-borne viral disease affecting humans, is transmitted by bite of mosquitoes genus Aedes and caused one four distinct serotypes dengue virus (DENV-1, -2, -3 -4). Infection with provides lifelong homotypic immunity. However, immunity against heterologous transient. As a consequence, secondary infection may lead to severer manifestations due cross-reactivity antibodies T-cells. Over 500,000 people are hospitalized every year around 2,5 million,...
Dengue virus (DENV) infection by one of the four serotypes (DENV-1 to 4) may result in a wide spectrum clinical manifestations, with unpredictable evolution and organ involvement. Due its association severe epidemics DENV-2 has been substantially investigated. In fact, first emergence new lineage Asian/American genotype Brazil (Lineage II) 2008 was associated cases increased mortality related A major challenge for dengue pathogenesis studies suitable animal model, but use immune-competent...
Dengue virus type 2 (DENV-2) is, traditionally, the most studied serotype due to its association with explosive outbreaks and severe cases. In Brazil, almost 20 years after first introduction in 1990s, a new lineage (Lineage II) of DENV-2 Asian/American genotype emerged caused an epidemic cases hospitalizations. Severe dengue includes multiple organ failure, renal involvement can be potentially related increased mortality. order better understand role DENV infection injury, here we aimed...
The lack of an experimental animal model for the study dengue pathogenesis is a limiting factor development vaccines and drugs. In previous studies, our group demonstrated susceptibility BALB/c mice to infection by virus (DENV) 1 2, was successfully isolated in several organs. this study, were experimentally infected intravenously with DENV-4, samples their saliva collected. Viral RNA extracted from subjected qRT-PCR, detection limit 0.002 PFU/mL. presence DENV-4 viral detected two mice,...
Dengue (DEN) is the most prevalent arbovirus among humans, and four billion people live at risk of infection. The clinical manifestations DEN are variable, disease may present subclinically or asymptomatically. A quarter patients develop classical dengue (CD) severe (SD), which potentially lethal involves vascular permeability changes, hemorrhage organ damage. involvement liver a fairly common feature in DEN, alterations range from asymptomatic elevation transaminases to acute failure. Since...
Abstract The coronaviruses (CoVs) called the attention of world for causing outbreaks severe acute respiratory syndrome (SARS-CoV), in Asia 2002-03, and disease Middle East (MERS-CoV), 2012. In December 2019, yet again a new coronavirus (SARS-CoV-2) first identified Wuhan, China, was associated with infection, known today as COVID-19. This virus is highly transmissible, quickly spread throughout China 30 additional countries. As result, World Health Organization (WHO) elevated status...
Ever since its brief introduction in the Brazilian territory 1981, dengue virus serotype 4 (DENV-4) remained absent from national epidemiological scenario for almost 25 years. The emergence of DENV-4 2010 resulted epidemics most states. DENV-4, however, remains one least studied among four DENV serotypes. Despite being known as a mild serotype, is associated with severe cases and deaths deserves to be investigated; lack suitable experimental animal models limiting factor pathogenesis...
Dengue is a disease caused by dengue virus (DENV-1 through -4). Among the four serotypes, DENV-4 remains least studied. Acute kidney injury potential complication of generally associated with severe infection.The goal this study was to investigate alterations experimental infection in adult BALB/c mice.In study, mice were infected intravenous route strain, isolated from human patient. The kidneys procured and subject histopathological ultrastructural analysis.The presence viral antigen...
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants concern (VOCs) isolated in Brazil 2021, hypothesizing a correlation between cellular alterations and mortality viral load transmissibility. For this purpose, reference isolates Alpha, Gamma, Zeta, Delta were inoculated into monolayers Vero-E6 cells. Viral RNA was quantified cell supernatants by RT‒PCR, infected cells analyzed Transmission Electron Microscopy (TEM) for qualitative quantitative evaluation...
Objective: To report a case of SARS-CoV-2/HIV coinfection in patient with history recent lung infections who had cardiopulmonary arrest on the day hospitalization for COVID-19 treatment, dying some days after multiple organ failure. Case detail: A 33-year-old immunosuppressed male (abandoning treatment HIV) was admitted to emergency department respiratory symptoms and tested positive SARS-CoV-2. During hospitalization, cardiorespiratory presented robust inflammatory profile, fungal...