A5089292980- Diphtheria, Corynebacterium, and Tetanus
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Gut microbiota and health
- Soybean genetics and cultivation
- Molecular Biology Techniques and Applications
- Transgenic Plants and Applications
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Salmonella and Campylobacter epidemiology
- Bioinformatics and Genomic Networks
- Veterinary medicine and infectious diseases
- Listeria monocytogenes in Food Safety
- Microbial infections and disease research
- Genetically Modified Organisms Research
- Microbial Natural Products and Biosynthesis
- Machine Learning in Bioinformatics
- Microbial Metabolic Engineering and Bioproduction
- Antimicrobial Peptides and Activities
- Fish biology, ecology, and behavior
- Insect-Plant Interactions and Control
- Microscopic Colitis
- Immune Response and Inflammation
- Gastrointestinal motility and disorders
Universidade Federal de Minas Gerais
2016-2022
Microbiologie de l’alimentation au service de la santé
2017-2022
Université Paris-Saclay
2017
Universidade Federal de Viçosa
2016
Faecalibacterium prausnitzii and its supernatant showed protective effects in different chemically-induced colitis models mice. Recently, we described 7 peptides found the F. supernatant, all belonging to a protein called Microbial Anti-inflammatory Molecule (MAM). These were able inhibit NF-κB pathway vitro anti-inflammatory properties vivo DiNitroBenzene Sulfate (DNBS)-induced model. This current paper tested MAM effect on vivo, using transgenic model of mice producing luciferase under...
Corynebacterium pseudotuberculosis is a pathogen classified into two biovars: C. biovar ovis, the etiologic agent of caseous lymphadenitis and equi, which causes ulcerative lymphangitis. The available whole genome sequences different strains have enabled identify difference genes related both virulence physiology each biovar. To evaluate be this could reflect at proteomic level to better understand shared factors exclusive ones ovis equi strains, we applied label-free quantitative...
Corynebacterium pseudotuberculosis is classified into two biovars, nitrate-negative biovar Ovis which the etiologic agent of caseous lymphadenitis in small ruminants and nitrate-positive Equi, causes abscesses ulcerative lymphangitis equines. The aim this study was to develop a quadruplex PCR assay that would allow simultaneous detection biovar-typing C. pseudotuberculosis. In present study, genomes strains were used identify genes involved nitrate reduction pathway improve species...
Based on the ability of nitrate reductase synthesis, Corynebacterium pseudotuberculosis is classified into two biovars: Ovis and Equi. Due to presence reductase, Equi biovar can survive in absence oxygen. On other hand, that does not have able adapt various ecological niches grow certain carbon sources. Apart from these biovars, some strains are also carry out reduction nitrate. The enzymes involved electron transport chain identified by silico methods. Findings about pathogen metabolism...
Summary Lactococcus lactis is a bacteria with high biotechnological potential, where frequently used in the amino acid production and of fermented dairy products, as well drug delivery systems mucosal vaccine vector. The knowledge functional core proteome important extremely for both fundamental understanding cell functions synthetic biology applications. In this study, we characterized L. lacits from proteomic analysis four strains : subsp. NCDO 2118, IL 1403, cremoris NZ 9000 MG 1363. Our...
Abstract With its antimicrobial and immunomodulating properties, the cathelicidin (LL37) plays an important role in innate immune system. Here, we attempted to alleviate chemically induced colitis using a lactococci strain that either directly expressed precursor LL37, hCAP18 (LL-pSEC:hCAP18), or delivered cDNA host cells under control of cytomegalovirus promoter (LL-Probi-H1:hCAP18). We also investigated whether alleviation symptoms could be explained through modification gut microbiota by...
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and secrete large amounts of listeriolysin O (LLO) in order improve potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO both cytoplasmic extracellular compartments, with higher quantities found culture supernatants. hemolytic assay showed recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing L....
The enzymes 1,2-diacylglycerol cholinephosphotrans-ferase (CPT) and lysophosphatidylcholine acyltransferase (LPCAT) are important in lipid metabolism soybean seeds. Thus, understand-ing the genes that encode these may enable their modification aid improvement of oil quality. In soybean, encoding have not been completely described; there-fore, this study aimed to identify, characterize, analyze silico expression soybean. We identified two gene models CPT LPCAT, one which presented an...
Spontaneous abortions, which occur in 15-20% of all pregnancies, constitute the most common human genetic disease, since miscarriages are caused by chromosomal anomalies.In our laboratory, we devised a protocol for sequential use quantitative fluorescent PCR (QF-PCR), homologous gene (HGQ-PCR) and SNP-arrays that allows examination numerical structural analyses chromosomes spontaneous abortions at low cost.We describe results with 1,295 samples fetal tissues collected consecutively after...