- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Burkholderia infections and melioidosis
- Diphtheria, Corynebacterium, and Tetanus
- Escherichia coli research studies
- Leptospirosis research and findings
- Vector-Borne Animal Diseases
- Mycobacterium research and diagnosis
- Milk Quality and Mastitis in Dairy Cows
- Antimicrobial Resistance in Staphylococcus
- Animal Diversity and Health Studies
- Galectins and Cancer Biology
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Veterinary medicine and infectious diseases
- Rural Development and Agriculture
- Animal health and immunology
- Rabies epidemiology and control
- Cardiovascular Conditions and Treatments
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Animal Nutrition and Physiology
Universidade Federal de Lavras
2016-2025
Universidade Federal de Minas Gerais
2011-2017
Brucella abortus S19 and RB51 strains have been successfully used to control bovine brucellosis worldwide; however, currently, most of our understanding the protective immune response induced by vaccination comes from studies in mice. The aim this study was characterize compare responses cattle prime-immunized with B. or revaccinated RB51. Female calves, aged 4 8 months, were vaccinated either vaccine (0.6–1.2 x 1011 CFU) (1.3 1010 on day 0, 365 experiment. Characterization performed using...
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine field strains, one type strain (ATCC 19410T) and vaccine (1002) were fingerprinted using ERIC-1R ERIC-2 primers, ERIC-1R+ERIC-2 primer pair. Twenty-nine genotypes generated by ERIC 1-PCR, 28 2-PCR 35 1+2-PCR. discriminatory index calculated 1, 2, 1+2-PCR 0.89, 0.86, 0.92,...
A cross sectional study was performed to assess the epidemiological status of bovine tuberculosis in state Minas Gerais, Brazil, 2013. The divided into seven regions, and a preset number herds randomly sampled each region. From farm, female cattle aged 24 months or older were subjected comparative cervical tuberculin test (CCTT). Animals with inconclusive results re-tested same diagnostic procedure after minimum interval 60 days. total 31?832 animals tested from 2?182 farms. An questionnaire...
This study aimed to determine the susceptibility profile of Brazilian Brucella abortus isolates from cattle eight antimicrobial agents that are recommended for treatment human brucellosis and correlate patterns with origin, biotype MLVA16-genotype strains. Screening 147 B. strains showed 100% sensitivity doxycycline ofloxacin, one (0.68%) strain resistant ciprofloxacin, two (1.36%) streptomycin, trimethoprim-sulfamethoxazole five (3.40%) gentamicin. For rifampicin, three (2.04%) were 54...
ABSTRACT: The aim of this study was to assess the prevalence and incidence canine leptospirosis brucellosis in Parque Francisco de Assis, a shelter Lavras, Minas Gerais State, Brazil, as well risk factors potentially associated with both diseases. Samples blood, urine, sera from all animals were collected 2019 (n = 329) 2020 325 dogs). DNA Leptospira spp. (urine) Brucella (urine blood) searched by PCR, whereas microagglutination test (MAT) agar gel immunodiffusion assay (AGID) performed...
Brucellosis is a worldwide distributed zoonosis that causes important economic losses to animal production. In Brazil, information on the distribution of biovars and genotypes Brucella spp. scarce or unavailable. This study aimed (i) biotype genotype 137 Brazilian cattle isolates (from 1977 2008) B. abortus (ii) analyze their distribution. 1, 2 3 (subgroup 3b) were confirmed 4 6 first described in Brazil. Genotyping by panel 1 revealed two groups, one clustering around 40 another 28. Panels...
Brucellosis caused by Brucella abortus is one of the most important zoonoses in world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies B. infection. Thus, present study aimed (i) evaluate genetic diversity isolates from brucellosis outbreak, and (ii) investigate vivo stability MLVA16 markers. Three-hundred seventy-five clinical samples, including 275 vaginal swabs 100 milk were cultured outbreak cattle herd,...
The aim of this study was to evaluate the viability Campylobacter spp. in frozen and chilled broiler carcasses using real-time PCR with propidium monoazide (PMA) pretreatment. Sixty were collected: 30 chilled. Each carcass submitted 2 protocols detect quantify spp.: one pretreatment PMA, which blocks amplification DNA from dead bacteria, other without PMA. results showed that PMA-pretreated carcasses, either or chilled, had a lower positivity rate compared untreated samples (P < 0.001)....
Corynebacterium pseudotuberculosis can be classified into two biovars or based on their nitrate-reducing ability. Strains isolated from sheep and goats show negative nitrate reduction are termed biovar Ovis, while strains horse cattle exhibit positive called Equi. However, molecular evidence has not been established so far to understand this difference, specifically if these C. under an evolutionary process. The ERIC 1 + 2 Minimum-spanning tree 367 of showed that the great majority Ovis...