A5000261172- Probiotics and Fermented Foods
- Tuberculosis Research and Epidemiology
- Inflammatory Bowel Disease
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Escherichia coli research studies
- Helicobacter pylori-related gastroenterology studies
- Infant Nutrition and Health
- Viral gastroenteritis research and epidemiology
- Pharmacological Effects of Natural Compounds
- Digestive system and related health
- Diabetes and associated disorders
- Receptor Mechanisms and Signaling
- vaccines and immunoinformatics approaches
- Microscopic Colitis
- Microbial Metabolism and Applications
- GABA and Rice Research
- Vagus Nerve Stimulation Research
- Antimicrobial Peptides and Activities
- Salmonella and Campylobacter epidemiology
- Microbial Metabolic Engineering and Bioproduction
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
Universidade Federal de Minas Gerais
2013-2023
Many probiotic bacteria have been described as promising tools for the treatment and prevention of inflammatory bowel diseases (IBDs). Most these are lactic acid bacteria, which part healthy human microbiota. However, little is known about effects transient present in normal diets, including Lactococcus lactis.In study, we analysed immunomodulatory three L. lactis strains vitro using intestinal epithelial cells. NCDO 2118 was administered 4 days to C57BL/6 mice during remission period...
Current immunological bioinformatic approaches focus on the prediction of allele-specific epitopes capable triggering immunogenic activity. The major histocompatibility complex (MHC) class I is well studied, and various software solutions exist for this purpose. However, currently available tools do not account concentration epitope products in mature protein product its relation to reliability target selection. We developed a computational strategy based measuring epitope's protein, called...
DNA vaccines are the third generation based on purified plasmid preparations containing transgenes that encode antigenic/therapeutic proteins or peptides capable of triggering an immune response against a wide range diseases. This vaccine platform presents several attributes confer distinct advantages over other technologies in terms safety, ease fabrication and stability. Many aspects, such as antigen expression especially vector design, under study because their great influence...
Interleukin-10 (IL-10) is the most important anti-inflammatory cytokine at intestinal level, and its absence involved in inflammatory bowel diseases. However, oral treatment with IL-10 difficult because of low survival gastrointestinal tract systemic treatments lead to undesirable side effects. The aim this paper was evaluate effect administration milks fermented by <i>Lactococcus lactis </i>strains that produce under control xylose-inducible expression system using a...
Inflammatory bowel diseases (IBD) are intestinal disorders characterized by inflammation in the gastrointestinal tract. Interleukin-10 is one of most important anti-inflammatory cytokines involved immune system and because its role downregulating inflammatory cascades, potential for IBD therapy under study. We previously presented development an invasive strain Lactococcus lactis (L. lactis) producing Fibronectin Binding Protein A (FnBPA) which was capable delivering, directly to host cells,...
Inflammatory bowel diseases are characterized by chronic intestinal inflammation that leads to severe destruction of the mucosa. Therefore, understanding their aetiology as well development new medicines is an important step for treatment such diseases. Consequently, Lactococcus lactis strains capable delivering a eukaryotic expression vector encoding interleukin 4 (IL-4) Mus musculus would represent strategy elaboration more effective alternative therapy against Crohn's disease.The murine...
Anti-Tumor Necrosis Factor-alpha therapy has become clinically important for treating inflammatory bowel disease. However, the use of conventional immunotherapy requires a systemic exposure patients and collateral side effects. Lactic acid bacteria have been shown to be effective as mucosal delivering system cytokine single domain antibodies, it is amenable clinical purposes. Therefore, lactic may function vehicles delivery therapeutic antibodies molecules gastrointestinal tract restricting...
Lactococcus lactis subsp. NCDO 2118 is a nondairy lactic acid bacterium, xylose fermenter, and gamma-aminobutyric (GABA) producer isolated from frozen peas. Here, we report the complete genome sequence of L. 2118, strain with probiotic potential activity.
Tuberculosis (TB) remains a major threat throughout the world and in 2015 it caused death of 1.4 million people. The Bacillus Calmette-Guérin (BCG) is only existing vaccine against this ancient disease; however, does not provide complete protection adults. New vaccines TB are eminently global priority. use bacteria as vehicles for delivery plasmids promising vaccination strategy. In study, we evaluated of, an engineered invasive Lactococcus lactis (expressing Fibronectin Binding Protein A...
Abstract Background Inflammatory bowel diseases (IBD) are intestinal disorders characterized by inflammation in the gastrointestinal tract (GIT) and to date, no efficient treatments exist. Interleukin-10 (IL-10), one of most important anti-inflammatory cytokines immune response, has been under study due its potential for IBD therapy; however, systemic lead undesirable side effects oral administration is limited quick degradation. To avoid these bottlenecks, we previously engineered an...
Inflammatory bowel diseases (IBD), such as Crohn's disease and ulcerative colitis, is characterized by extensive inflammation due to dysregulation of the innate adaptive immune system whose exact etiology not yet completely understood. Currently there no cure for IBD, thus search new molecules capable controlling IBD their delivery site are goal many researchers. The aim this work was evaluate anti-inflammatory effect administration milks fermented a Lactococcus (L.) lactis strain producing...
Lactococcus lactis (L. lactis), a generally regarded as safe (GRAS) bacterium has recently been investigated mucosal delivery vehicle for DNA vaccines. Because of its GRAS status, L. represents an attractive alternative to attenuated pathogens. Previous studies showed that eukaryotic expression plasmids could be delivered into intestinal epithelial cells (IECs) by lactis, or recombinant invasive strains leading heterologous protein expression. Although antigens in IECs might lead vaccine...
Type 1 diabetes (T1D) is an autoimmune disease that culminates in beta cell destruction the pancreas and, subsequently, deficiency insulin production. Cytokines play a crucial role development of diabetes, orchestrating recruitment and action immune cells, to not only destroy insulin-producing cells but also preserve them. Therefore, aim this study was investigate effect orally administered Lactococcus lactis MG1363 FnBPA+ strains carrying plasmids encoding IL-4 IL-10 streptozotocin- (STZ-)...
A regimen utilizing Bacille Calmette-Guerin (BCG) and another vaccine system as a booster may represent promising strategy for the development of an efficient tuberculosis adults. In previous work, we confirmed ability Lactococcus lactis fibronectin-binding protein (FnBPA+) (pValac:ESAT-6), live mucosal DNA vaccine, to produce specific immune response in mice after oral immunization. this study, examined immunogenicity strain BCG mice.After immunization, cytokine immunoglobulin profiles were...
Abstract Intestinal fibrosis associated with Crohn’s disease (CD), which a common and serious complication of inflammatory bowel diseases. In this context, heat shock proteins (HSPs) might serve as an alternative treatment because these antigens play important roles in the regulation effector T cells. We thus evaluated anti-inflammatory antifibrotic capacities invasive Hsp65-producing strain— Lactococcus lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65)—in chronic intestinal inflammation to assess its...
The development of a new vaccine strategy against tuberculosis is urgently needed and has been greatly encouraged by the scientific community worldwide. In this work, we constructed lactococcal DNA based on fusion two Mycobacterium antigens, ESAT-6 Ag85A, examined its immunogenicity. coding sequences Ag85A genes were fused cloned into eukaryotic expression pValac vector, functionality vector was confirmed in vitro . Then, L. lactis FnBPA + (pValac: e6ag85a ) obtained used for oral...
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and secrete large amounts of listeriolysin O (LLO) in order improve potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO both cytoplasmic extracellular compartments, with higher quantities found culture supernatants. hemolytic assay showed recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing L....
To investigate the anti-inflammatory activity of an invasive and Hp65-producing strain Lactococcus lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65) in acute 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis mice as innovative therapeutic strategy against Crohn's disease (CD).The pXYCYT:Hsp65 plasmid was transformed into L. strain, resulting strain. Then, functionality evaluated vitro for Hsp65 production by Western blotting invasion Caco-2 cells. The results demonstrated that able to produce...
It is well known that cholinergic hypofunction contributes to cardiac pathology, yet, the mechanisms involved remain unclear. Our previous study has shown genetically engineered model of deficit, vesicular acetylcholine transporter knockdown homozygous (VAChT KDHOM) mice, exhibit pathological remodeling and a gradual increase in mass with aging. Given an often caused by adrenergic hyperactivity, we hypothesized VAChT KDHOM mice might have norepinephrine (NE) levels. We thus investigated...