A5076891526- Pulmonary Hypertension Research and Treatments
- Ion channel regulation and function
- Nitric Oxide and Endothelin Effects
- Electrospun Nanofibers in Biomedical Applications
- Ion Transport and Channel Regulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Function and Risk Factors
- Asthma and respiratory diseases
- Polymer Surface Interaction Studies
- Metabolomics and Mass Spectrometry Studies
- Protease and Inhibitor Mechanisms
- 3D Printing in Biomedical Research
- Livestock Management and Performance Improvement
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Animal Genetics and Reproduction
- Hydrogels: synthesis, properties, applications
- Agricultural Economics and Practices
- Agricultural Systems and Practices
- Graphene and Nanomaterials Applications
- Adenosine and Purinergic Signaling
- Liver Disease and Transplantation
- Connexins and lens biology
- ATP Synthase and ATPases Research
- Venous Thromboembolism Diagnosis and Management
Ludwig Boltzmann Institute for Lung Vascular Research
2016-2025
Medical University of Graz
2009-2025
Semmelweis University
2024
BioTechMed-Graz
2023
Institute of Medical Sciences
2018
Ludwig Boltzmann Institute for Digital Health and Prevention
2014-2015
Directorate of Poultry Research
2015
Veterinary & Animal Husbandry
2015
University of Graz
2014
Jiangsu University
2011
Chronic thromboembolic pulmonary hypertension (CTEPH) is associated with chronic inflammation but the pathological mechanisms are largely unknown. Our study aimed to simultaneously profile a broad range of cytokines in supernatant endarterectomy (PEA) surgical material, as well prospectively patients CTEPH investigate whether circulating haemodynamic and physical characteristics patients. Herein, we show that PEA specimens revealed significant upregulation interleukin (IL)-6, monocyte...
The potassium channel TWIK-related acid sensitive (TASK)-1 channel, together with other channels, controls the low resting tone of pulmonary arteries. Src family tyrosine kinase (SrcTK) may control function in human artery smooth muscle cells (hPASMCs) response to changes oxygen tension and clinical use a SrcTK inhibitor has resulted partly reversible hypertension. This study aimed determine role hypoxia-induced inhibition channels hPASMCs. We show that is co-localised TASK-1 channel....
Our systematic analysis of anion channels and transporters in idiopathic pulmonary arterial hypertension (IPAH) showed marked upregulation the Cl − channel TMEM16A gene. We hypothesised that overexpression might represent a novel vicious circle molecular pathways causing (PAH). investigated healthy donor lungs (n=40) recipient with IPAH (n=38) for expression transporter genes small arteries artery smooth muscle cells (PASMCs). In IPAH, was strongly upregulated patch-clamp recordings...
Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare disease with persistent thrombotic occlusion or stenosis of the large arteries resulting in hypertension. Surgical removal neointimal layer these vessels together non-resolved thrombus consisting organized collagen-rich fibrotic areas partly recanalized regions treatment choice (pulmonary endarterectomy, PEA). The present study investigates endothelial cells isolated from such material as well factors surgical PEA material,...
Abstract 3D printing of bio‐based nanomaterials into complex structures with design flexibility, structural anisotropy, and long‐term stability is a key issue for biomedical applications. Herein, 3D‐printed ionically crosslinked anisotropic, water‐proof, tunable mechanical properties are fabricated using polysaccharide ink composed nanocellulose, alginate, CaCO 3 nanoparticles. The excellent shear thinning the ink, combined double or even triple extrusion, allow (tubes, buckets, ears, boat...
Lung vessel remodeling leads to increased pulmonary vascular resistance, causing arterial hypertension (PAH), and consequently right ventricular hypertrophy failure. In patients suffering from systemic sclerosis (SSc), PAH can occur is a life-threatening complication. Dysregulation of immune processes plays crucial role in remodeling, as has previously been shown Fos-related antigen-2 (Fra-2) transgenic (TG) mice, model SSc-PAH. Here, we investigate whether the Fra-2 TG driven by type 2...
This study enhances the bioactivity of polycaprolactone (PCL) scaffolds for tissue engineering by functionalizing them with oxidized hyaluronic acid glycine-peptide conjugates to improve endothelial cell adhesion and growth. Hyaluronic was conjugated a create bioactive interface on PCL (static water contact angle, SCA(H2O): 98°). The were fabricated using melt extrusion 3D printing technique. HA-glycine peptide immobilized aminolyzed via Schiff-base chemistry, introducing hydrophilicity...
Section:ChooseTop of pageAbstract <<MATERIALS AND METHODSRESULTSDISCUSSIONReferencesCITING ARTICLES
Lung cancer is the leading cause of deaths worldwide; survival times are poor despite therapy. The role two-pore domain K+ (K2P) channel TASK-1 (KCNK3) in lung at present unknown. We found that expressed non-small cell (NSCLC) lines variable levels. In a highly expressing NSCLC line, A549, characteristic pH- and hypoxia-sensitive non-inactivating current was measured, indicating presence functional channels. Inhibition led to significant depolarization these cells. Knockdown by siRNA...
The tryptophan metabolite kynurenine is significantly increased in pulmonary arterial hypertension (PAH) patients, and it a potent vasodilator of systemic arteries. Our aim was to investigate the role circulation. Serum tryptophan, kynurenine, kynurenic acid levels were measured 20 idiopathic PAH (IPAH) healthy controls, patients with chronic lung disease or metabolic syndrome without PH. Laser-dissected arteries from IPAH control lungs tested for expression indoleamine-2, 3-dioxygenase...
Tumor cells use broad spectrum proteolytic activity of plasmin to invade tissue and form metastatic foci. Cell surface-associated enolase-1 (ENO-1) enhances formation thus participates in the regulation pericellular proteolysis. Although increased levels cell surface bound ENO-1 have been described different types cancer, molecular mechanism responsible for exteriorization remains elusive. In present study, protein were found ductal breast carcinoma on highly cancer line MDA-MB-231. Elevated...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Abstract: Prostacyclin analogues are standard therapeutic options for vasoconstrictive diseases, including pulmonary hypertension and Raynaud's phenomenon. Although effective, these treatment strategies expensive have several side effects. To improve drug efficiency, we tested liposomal nanoparticles as carrier systems. In this study, synthesized tailored the prostacyclin analogue iloprost evaluated their pharmacologic efficacy on mouse intrapulmonary arteries, using a wire myograph. The use...
This work describes the interaction of human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- nanopatterned polymer interfaces. Protein adsorption studies were correlated fibrin clotting time growth primary pulmonary artery endothelial cells (hECs) on these patterns. It was observed that blends polycaprolactone (PCL) trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending mass ratio polymers in spinning solutions. Vapor-phase...
Introduction A reduction in pulmonary artery relaxation is a key event the pathogenesis of arterial hypertension (PAH). Cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction airway epithelial cells plays central role cystic fibrosis; CFTR also expressed arteries and has been shown to control endothelium-independent relaxation. Aim objectives We aimed delineate PAH through observational interventional experiments human tissues animal models. Methods results...
The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches. adaptation use biobased materials for applications such as targeted therapeutic is, therefore, in high demand. A crucial part this relates porous hollow structures that are biocompatible, pH-responsive, deliver active substances, contribute pain relief, wound healing, tissue regeneration, so forth. In study, we developed a facile single-step water-based method fabrication...
Hybrid collagen (Coll) bioscaffolds have emerged as a promising solution for tissue engineering (TE) and regenerative medicine. These innovative combine the beneficial properties of Coll, an important structural protein extracellular matrix, with various other biomaterials to create platforms long-term cell growth formation. The integration or cross-linking Coll increases mechanical strength stability introduces tailored biochemical physical factors that mimic natural microenvironment. This...
Cardioprotective benefits of ω-3 fatty acids such as docosahexaenoic acid (DHA) are well established, but the regulatory effect DHA on vascular tone and pressure in pulmonary hypertension is largely unknown. As a potent regulator K + channels, we hypothesised that modulates membrane potential artery smooth muscle cells (PASMCs) through channels thus exerts its effects pressure. We show caused dose-dependent activation calcium-activated (KCa) current primary human PASMCs endothelium-dependent...
Polymer-based biomaterials particularly polycaprolactone (PCL) are one of the most promising substrates for tissue engineering. The surface chemistry these materials plays a major role since it governs protein adsorption, cell adhesion, viability, degradation, and biocompatibility in first place. This study correlates interaction abundant serum proteins (albumin, immunoglobulins, fibrinogen) with properties PCL its influence on morphology metabolic activity primary human arterial endothelial...
The multi-kinase inhibitor dasatinib is used for treatment of imatinib-resistant chronic myeloid leukemia, but prone to induce microvascular dysfunction. In lung this can manifest as capillary leakage with pleural effusion, pulmonary edema or even arterial hypertension. To understand how causes endothelial dysfunction we examined the effects clinically relevant concentrations on both human macro- and cells (ECs). was compared imatinib nilotinib, two other BCR/Abl kinase inhibitors that do...
Endothelial dysfunction is one of the hallmarks different vascular diseases, including pulmonary arterial hypertension (PAH). Ion channelome changes have long been connected to remodeling in PAH, yet only recently has focus shifted towards Ca2+-activated Cl− channels (CaCC). The most prominent member CaCC TMEM16A shown contribute pathogenesis idiopathic PAH (IPAH) smooth muscle cells, however its role homeostasis healthy human endothelial cells (PAECs) and development remains...