A5086552188- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Bacterial Identification and Susceptibility Testing
- Neonatal Respiratory Health Research
- Prion Diseases and Protein Misfolding
- Yersinia bacterium, plague, ectoparasites research
- Mechanical Circulatory Support Devices
- Bacillus and Francisella bacterial research
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Infrared Thermography in Medicine
- Metabolomics and Mass Spectrometry Studies
- Respiratory Support and Mechanisms
- Thermography and Photoacoustic Techniques
- Enterobacteriaceae and Cronobacter Research
- Probiotics and Fermented Foods
- Advanced Proteomics Techniques and Applications
- Trace Elements in Health
- Planetary Science and Exploration
- Mycobacterium research and diagnosis
- Identification and Quantification in Food
- Microbial Community Ecology and Physiology
- Plant-Microbe Interactions and Immunity
- Mass Spectrometry Techniques and Applications
- Congenital Heart Disease Studies
Robert Koch Institute
2016-2025
Klinikum Bremen-Mitte
2007-2021
ORCID
2021
Universität Hamburg
2007-2010
University Medical Center Hamburg-Eppendorf
2007-2010
Radboud University Nijmegen
2010
Radboud University Medical Center
2010
University Cancer Center Hamburg
2010
Federal Institute for Risk Assessment
2010
Society of Paediatric Oncology and Haematology
2008
ABSTRACT Bacillus amyloliquefaciens FZB42 is a Gram-positive plant growth-promoting bacterium with an impressive capacity to synthesize nonribosomal secondary metabolites antimicrobial activity. Here we report on novel circular bacteriocin which ribosomally synthesized by FZB42. The compound displayed high antibacterial activity against closely related bacteria. Transposon mutagenesis and subsequent site-specific combined matrix-assisted laser desorption ionization–time of flight mass...
The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a manner that enables efficient protein digestion for subsequent mass spectrometric analysis. Today's strategies are commonly conceptualized around the removal detergents, which essential extraction but strongly interfere with and LC-MS. These multi-step preparations contribute lack reproducibility as they prone losses, biases contaminations, while being time-consuming labor-intensive. We report...
Abstract Infrared nanospectroscopy enables novel possibilities for chemical and structural analysis of nanocomposites, biomaterials or optoelectronic devices. Here we introduce hyperspectral infrared nanoimaging based on Fourier transform with a tunable bandwidth-limited laser continuum. We describe the technical implementations present near-field images about 5,000 pixel, each one covering spectral range from 1,000 to 1,900 cm −1 . To verify technique demonstrate its application potential,...
The last two decades have seen tremendous progress in the application of two-dimensional correlation spectroscopy (2D-COS) as a versatile analysis method for data series obtained using large variety different spectroscopic modalities, including infrared (IR) and Raman spectroscopy. technique is applicable to spectra recorded under influence an external sample perturbation. Two-dimensional COS not only helpful decipher correlations, which may exist between distinct spectral features, but can...
BIOMEX (BIOlogy and Mars EXperiment) is an ESA/Roscosmos space exposure experiment housed within the facility EXPOSE-R2 outside Zvezda module on International Space Station (ISS). The design of multiuser supports—among others—the investigations into stability level degradation space-exposed biosignatures such as pigments, secondary metabolites, cell surfaces in contact with a terrestrial analog mineral environment. In parallel, analysis viability investigated organisms has provided relevant...
Identification of microorganisms by Fourier transform infrared (FT-IR) spectroscopy is known as a promising alternative to conventional identification techniques in clinical, food, and environmental microbiology. In this study we demonstrate the application FT-IR hyperspectral imaging for rapid, objective, cost-effective diagnosis pathogenic bacteria. The proposed method involves relatively short cultivation step under standardized conditions, transfer microbial material onto suitable IR...
This report demonstrates the applicability of a combination matrix-assisted laser desorption ionization-time flight (MALDI-TOF) mass spectrometry (MS) and chemometrics for rapid reliable identification vegetative cells causative agent anthrax, Bacillus anthracis. cultures were prepared under standardized conditions inactivated according to recently developed MS-compatible inactivation protocol highly pathogenic microorganisms. MALDI-TOF MS was then employed collect spectra from microbial...
Identification of microorganisms, specifically vegetative cells and spores, by intact cell mass spectrometry (ICMS) is an emerging new technology. The technique provides specific biomarker profiles which can be employed for bacterial identification at the genus, species, or even subspecies level holding potential to serve as a rapid sensitive in clinical food microbiology also detection biosafety (BSL) 3 microorganisms. However, development ICMS BSL-3 microorganisms hampered fact that no...
In the case of a release highly pathogenic bacteria (HPB), there is an urgent need for rapid, accurate, and reliable diagnostics. MALDI-TOF mass spectrometry relatively inexpensive technique that becoming increasingly important in microbiological diagnostics to complement classical microbiology, PCR, genotyping HPB. present study, results joint exercise with 11 partner institutions from nine European countries are presented. this exercise, 10 distinct microbial samples, among them five HPB,...
Hyperspectral imaging (HSI) techniques are useful for obtaining very detailed structural and compositional information from biomedical, pharmaceutical, or clinical samples, among others. The informative value of these methods can be further increased through the application different HSI joint analysis data. However, interpretation understanding multimodal have been impeded by difficulties in registration data sets lack integrative concepts. Here, we introduce two-dimensional correlation...
Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens also widely distributed natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria respiratory secretions Argentinean CF hospital settings, we found six isolates concatenated species-specific allele sequence that differed by more than 3 % those validly published names. According to...
The combination of short liquid chromatography (LC) gradients and data-independent acquisition (DIA) by mass spectrometry (MS) has proven its huge potential for high-throughput proteomics. However, the optimization isolation window schemes resulting in a certain number data points per peak (DPPP) is understudied, although it one most important parameters outcome this methodology. In study, we show that substantially reducing DPPP short-gradient DIA massively increases protein identifications...