A5020647499- Cannabis and Cannabinoid Research
- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Pancreatic function and diabetes
- Bacterial Infections and Vaccines
- Legionella and Acanthamoeba research
- Drug Transport and Resistance Mechanisms
- Adipose Tissue and Metabolism
- Amoebic Infections and Treatments
- Microbial Community Ecology and Physiology
- Pharmacological Receptor Mechanisms and Effects
- Salmonella and Campylobacter epidemiology
- Muscle metabolism and nutrition
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Pharmacological Effects and Toxicity Studies
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- Protein Degradation and Inhibitors
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Computational Drug Discovery Methods
- Bacteriophages and microbial interactions
Roche (Switzerland)
2018-2025
Spiez Laboratory
2014-2024
Bayer (Germany)
2017-2024
Pädagogische Hochschule FHNW
2022
Pädagogische Hochschule Bern
2022
FHNW University of Applied Sciences and Arts
2022
SIB Swiss Institute of Bioinformatics
2019
University Hospital of Bern
2018-2019
University of Bern
1998-2018
Leipzig University
2014-2018
Urinary tract infection (UTI) by uropathogenic Escherichia coli (UPEC) is one of the most common infections, particularly affecting women. The interaction FimH, a lectin located at tip bacterial pili, with high mannose structures critical for ability UPEC to colonize and invade bladder epithelium. We describe synthesis in vitro/in vivo evaluation α-d-mannosides block bacteria/host cell interaction. According pharmacokinetic properties, prodrug approach their UTI mouse model was explored. As...
The human multidrug and toxin extrusion (MATE) transporter 1 contributes to the tissue distribution excretion of many drugs. Inhibition MATE1 may result in potential drug-drug interactions (DDIs) alterations drug exposure accumulation various tissues. primary goals this project were identify inhibitors with clinical importance or vitro utility elucidate physicochemical properties that differ between OCT2 inhibitors. Using a fluorescence assay ASP(+) uptake cells stably expressing MATE1, over...
Vector control is the mainstay of malaria programmes. Successful vector profoundly relies on accurate information target mosquito populations in order to choose most appropriate intervention for a given species and monitor its impact. An impediment identify existence morphologically identical sibling that play different roles transmission pathogens parasites. Currently PCR diagnostics are used distinguish between species. based methods are, however, expensive, time-consuming their...
Monoacylglycerol lipase (MAGL) is the pivotal catabolic enzyme responsible for signal termination in endocannabinoid system. Inhibition of MAGL offers unique advantages over direct activation cannabinoid receptors treating cancer, metabolic disorders, and inflammatory diseases. Although specific fluorescent molecular imaging probes are commonly used real-time analysis localization distribution drug targets cells, they almost invariably composed a linker connecting pharmacophore with large...
The initial step for the successful establishment of urinary tract infections (UTIs), predominantly caused by uropathogenic Escherichia coli, is adhesion bacteria to urothelial cells. This attachment mediated FimH, a mannose-binding adhesin, which expressed on bacterial surface. To date, UTIs are mainly treated with antibiotics, leading ubiquitous problem increasing resistance against most currently available antimicrobials. Therefore, new treatment strategies urgently needed, avoiding...
The free-living amoeba Naegleria fowleri is the causative agent of rapidly progressing and typically fatal primary amoebic meningoencephalitis (PAM) in humans. Despite devastating nature this disease, which results > 97% mortality, knowledge pathogenic mechanisms incomplete. This work presents a comparative proteomic approach based on an experimental model potential N. trophozoites influenced by compositions different media. As scaffold for analysis, we sequenced genome transcriptome...
In the case of a release highly pathogenic bacteria (HPB), there is an urgent need for rapid, accurate, and reliable diagnostics. MALDI-TOF mass spectrometry relatively inexpensive technique that becoming increasingly important in microbiological diagnostics to complement classical microbiology, PCR, genotyping HPB. present study, results joint exercise with 11 partner institutions from nine European countries are presented. this exercise, 10 distinct microbial samples, among them five HPB,...
Age-related macular degeneration (AMD) is a leading cause of severe vision loss in the aged population. The etiology AMD multifactorial including nutritional factors, genetic variants mainly complement pathway, environmental risk factors and alterations intestinal microbiome. However, it remains unexplored whether there an interdependency these to development AMD. To investigate this issue, shotgun metagenomics analysis 57 neovascular 58 healthy controls as well 16 C3-deficient mice...
The elevation of endocannabinoid levels through inhibiting their degradation afforded neuroprotection in CaMKIIα-TDP-43 mice, a conditional transgenic model frontotemporal dementia. However, which cannabinoid receptors are mediating these benefits is still pending to be elucidated. We have investigated the involvement CB1 and CB2 receptor using chronic treatments with selective ligands analysis cognitive deterioration Novel Object Recognition test, immunostaining for neuronal glial markers...
It was hypothesized that transcriptional reprogramming is involved in the structural and functional adaptations of lipid metabolism human tibialis anterior muscle (TA) from endurance-trained male subjects. RT-PCR experiments demonstrated a significant upregulation mRNA level key enzymes 1) lipolytic mobilization fatty acids (FA) intramyocellular (IMCL) stores via hormone-sensitive lipase (LIPE), 2) FA transport acid binding protein (FABP3), 3) oxidative phosphorylation (cytochrome c oxidase...
It is believed that the induction of fos and jun gene family transcription factors might be at origin genetic events leading to differential regulation muscle-specific genes. We have investigated effect a 30-min running bout in untrained subjects on expression mRNAs all members families, including c- fos, fosB, fosBdel, fra-1, fra-2 as well jun, junB, junD. While were transiently upregulated 10- 20-fold (an exception being fra-2), was up 3-fold only. The could also demonstrated protein...
Metabolic stress is believed to constitute an important signal for training‐induced adjustments of gene expression and oxidative capacity in skeletal muscle. We hypothesized that the effects endurance training on muscle‐relevant transcripts ultrastructure would be specifically modified by a hypoxia complement during exercise due enhanced glycolytic strain. Endurance untrained male subjects conditions increased subsarcolemmal mitochondrial density recruited vastus lateralis muscle power...
Monoacylglycerol lipase (MAGL) regulates endocannabinoid 2-arachidonoylglycerol (2-AG) and eicosanoid signalling. MAGL inhibition provides therapeutic opportunities but clinical potential is limited by central nervous system (CNS)-mediated side effects. Here, we report the discovery of LEI-515, a peripherally restricted, reversible inhibitor, using high throughput screening medicinal chemistry programme. LEI-515 increased 2-AG levels in peripheral organs, not mouse brain. attenuated liver...
Using commercially available microarray technology, we investigated a series of transcriptional adaptations caused by atrophy rat m. soleus due to 35 days hindlimb suspension. We detected 395 out 1,200 tested transcripts, which reflected 1%-5% totally expressed genes. From various cellular functional pathways, multiple genes that spanned 200-fold range gene expression levels. Statistical analysis combining L1 regression with the sign test based on conservative Bonferroni correction...