A5067266133- Legionella and Acanthamoeba research
- Amoebic Infections and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Gut microbiota and health
- Oral microbiology and periodontitis research
- Retinal Diseases and Treatments
- Cancer Research and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Protist diversity and phylogeny
- Probiotics and Fermented Foods
- Allergic Rhinitis and Sensitization
- Mycobacterium research and diagnosis
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Salivary Gland Disorders and Functions
- Colorectal Cancer Screening and Detection
- Retinal and Optic Conditions
- Neurogenesis and neuroplasticity mechanisms
- Cytomegalovirus and herpesvirus research
University of Bern
2013-2024
University Hospital of Bern
2016-2024
Universitätsklinik für Augenheilkunde
2019
SIB Swiss Institute of Bioinformatics
2019
Spiez Laboratory
2013-2014
Age-related macular degeneration (AMD) is the most frequent cause of blindness in elderly. There evidence that nutrition, inflammation and genetic risk factors play an important role development AMD. Recent studies suggest composition intestinal microbiome associated with metabolic diseases through modulation host metabolism. To investigate whether compositional functional alterations are AMD, we sequenced gut metagenomes patients AMD controls. The genera Anaerotruncus Oscillibacter as well...
Abstract Background Dupilumab is used for the treatment of atopic dermatitis (AD). Approximately one third AD patients develop a dupilumab‐associated ocular surface disease (DAOSD), which pathomechanism poorly understood. This study aimed at investigating inflammatory markers in tear fluids on dupilumab therapy. Methods Tear were collected from with DAOSD (ADwDAOSD), without (ADw/oDAOSD), and non‐AD before during therapy, analyzed using specialized proteomic approach quantifying markers. The...
The free-living amoeba Naegleria fowleri is the causative agent of rapidly progressing and typically fatal primary amoebic meningoencephalitis (PAM) in humans. Despite devastating nature this disease, which results > 97% mortality, knowledge pathogenic mechanisms incomplete. This work presents a comparative proteomic approach based on an experimental model potential N. trophozoites influenced by compositions different media. As scaffold for analysis, we sequenced genome transcriptome...
Age-related macular degeneration (AMD) is a leading cause of severe vision loss in the aged population. The etiology AMD multifactorial including nutritional factors, genetic variants mainly complement pathway, environmental risk factors and alterations intestinal microbiome. However, it remains unexplored whether there an interdependency these to development AMD. To investigate this issue, shotgun metagenomics analysis 57 neovascular 58 healthy controls as well 16 C3-deficient mice...
The opportunistic pathogen Naegleria fowleri establishes infection in the human brain, killing almost invariably within 2 weeks. amoeba performs piece-meal ingestion, or trogocytosis, of brain material causing direct tissue damage and massive inflammation. cellular basis distinguishing N. from other species, which are all non-pathogenic, is not known. Yet, with geographic range advancing, potentially due to climate change, understanding how this invades kills both important timely.
Although dry eye disease (DED) is one of the most common ocular surface diseases worldwide, its pathogenesis incompletely understood, and treatment options are limited. There growing evidence that complex interactions between microbiome (OSM) tear fluid constituents, potentially leading to inflammatory processes, associated with such as DED. In this study, we aimed find unique compositional functional features OSM human microbial proteins in patients Applying whole-metagenome shotgun...
Purpose: The purpose of this study was to explore the interplay between ocular surface microbiome and tear proteome in humans order better understand pathogenesis surface-associated diseases. Methods: Twenty eyes from 20 participants were included study. sequenced by whole-metagenome shotgun sequencing using lid conjunctival swabs. Furthermore, identified chromatography tandem mass spectrometry. After compositional functional profiling metagenome characterization gene ontology, association...
Retinal artery occlusion (RAO) is a sight threatening complication of cardiovascular disease and commonly occurs due to underlying atherosclerosis. As atherosclerosis in particular has been associated with compositional alterations the gut microbiome, we investigated this association patients clinically confirmed non-arteritic RAO compared age- sex-matched controls. On phylum level, relative abundance Bacteroidetes was decreased controls, whereas opposite applied for Proteobacteria. Several...
Abstract Background The human gut microbiome (GM) is involved in inflammation and immune response regulation. Dysbiosis, an imbalance this ecosystem, facilitates pathogenic invasion, disrupts equilibrium, potentially triggers diseases including various leucocyte antigen (HLA)-B27-associated autoinflammatory autoimmune such as inflammatory bowel disease (IBD) spondyloarthropathy (SpA). This study assesses compositional functional alterations of the GM patients with HLA-B27-associated...
Abstract Background Bacterial meningitis caused by Streptococcus pneumonia e leads to death in up 30% of patients and leaves half the survivors with neurological sequelae. The inflammatory host reaction initiates induction kynurenine pathway contributes hippocampal apoptosis, a form brain damage that is associated learning memory deficits experimental paradigms. Vitamin B6 an enzymatic cofactor may thus limit accumulation neurotoxic metabolites preserve cellular energy status. aim this study...
Abstract Of the 40 described Naegleria species, only N. fowleri can establish infection in humans, killing almost invariably within two weeks. In brain, amoeba performs piece-meal ingestion, or trogocytosis, of brain material causing massive inflammation. Conversely, its close relative gruberi , which is used as a laboratory model organism, non-pathogenic. The exact pathogenicity factors distinguishing from harmless relatives are unclear. We have here taken an -omics approach to...
The intestinal microbiome plays a central role in human health and disease. While its composition is relatively stable throughout adulthood, the microbial balance starts to decrease later life stages. Thus, order maintain good quality of life, including prevention age-associated diseases elderly, it important understand dynamics microbiome. In this study, stool samples 278 participants were sequenced by whole metagenome shotgun sequencing their taxonomic functional profiles characterized....
The low microbial abundance on the ocular surface results in challenges characterization of its microbiome. purpose this study was to reveal factors introducing bias pipeline from sample collection data analysis low-abundant microbiomes.Lower conjunctiva and lower lid swabs were collected six participants using either standard cotton or flocked nylon swabs. Microbial DNA isolated with two different kits (with without prior host depletion mechanical lysis), followed by whole-metagenome...
Although glaucoma is a leading cause of irreversible blindness worldwide, its pathogenesis incompletely understood, and intraocular pressure (IOP) the only modifiable risk factor to target disease. Several associations between gut microbiome glaucoma, including IOP, have been suggested. There growing evidence that interactions microbes on ocular surface, termed surface (OSM), tear proteins, collectively called proteome, may also play role in diseases such as glaucoma. This study aimed find...
Abstract Background: Age-related macular degeneration (AMD) is a leading cause of severe vision loss in the aged population. The etiology AMD multifactorial and includes nutritional factors, genetic variants mainly complement pathway, environmental risk factors alterations intestinal microbiome. However, it remains largely unexplored whether there an interdependency these to development AMD. To investigate this issue, comprehensive shotgun metagenomics analysis 57 58 healthy controls as well...