A5049638970- Advanced Condensed Matter Physics
- Physics of Superconductivity and Magnetism
- Advanced NMR Techniques and Applications
- Toxoplasma gondii Research Studies
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Magnetic and transport properties of perovskites and related materials
- Parasitic Infections and Diagnostics
- X-ray Diffraction in Crystallography
- Solid-state spectroscopy and crystallography
- Radiomics and Machine Learning in Medical Imaging
- X-ray Spectroscopy and Fluorescence Analysis
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
- Crystallography and Radiation Phenomena
- Antimicrobial Peptides and Activities
- Atomic and Subatomic Physics Research
- Systemic Sclerosis and Related Diseases
- NMR spectroscopy and applications
- Insect symbiosis and bacterial influences
- Parasitic infections in humans and animals
- Magnetic properties of thin films
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Aquaculture disease management and microbiota
- Mosquito-borne diseases and control
University of Bern
2019-2024
Paul Scherrer Institute
2011-2017
Swiss Light Source
2015
University of Warwick
2008-2009
University of Cambridge
2008-2009
University of St Andrews
2008-2009
University of Zurich
2005
Human mesenchymal stromal cell (hMSC) secretomes have shown to influence the microenvironment upon injury, promoting cytoprotection, angiogenesis, and tissue repair. The angiogenic potential is of particular interest for treatment ischemic diseases. Interestingly, hMSC isolated from different sources dissimilarities with respect their profile. This study compares angiogenesis adipose (hADSCs), bone marrow (hBMSCs), umbilical cord Wharton's jelly (hWJSCs). were obtained under xenofree...
Background Radiomic features calculated from routine medical images show great potential for personalised medicine in cancer. Patients with systemic sclerosis (SSc), a rare, multiorgan autoimmune disorder, have similarly poor prognosis due to interstitial lung disease (ILD). Here, our objectives were explore computed tomography (CT)-based high-dimensional image analysis (“radiomics”) characterisation, risk stratification and relaying information on pathophysiology SSc-ILD. Methods We...
Weak hydrogen bonding in uracil and 4-cyano-4'-ethynylbiphenyl, for which single-crystal diffraction structures reveal close CH···OC C⋮CH···N⋮C distances, is investigated a study that combines the experimental determination of 1H, 13C, 15N chemical shifts by magic-angle spinning (MAS) solid-state NMR with first-principles calculations using plane-wave basis sets. An optimized synthetic route, including isolation characterization intermediates, to 4-cyano-4'-ethynylbiphenyl at natural...
The interaction between the endohedral unit in single-molecule magnet Dy_{2}ScN@C_{80} and a rhodium (111) substrate leads to alignment of Dy 4f orbitals. resulting orientation Dy_{2}ScN plane parallel surface is inferred from comparison angular anisotropy x-ray absorption spectra multiplet calculations corresponding ligand field. magnetic circular dichroism also angle dependent signals strong magnetocrystalline anisotropy. This directly relates geometric structure. Element specific...
Heteronuclear solid-state magic-angle spinning (MAS) NMR experiments for probing 15N−17O dipolar and J couplings are presented [2H(NH3),1-13C,15N,17O2]glycine·2HCl [15N2,17O2]uracil. Two-dimensional correlation spectra obtained using the R3-HMQC experiment; glycine·2HCl, intensity of resolved peaks C═O C−O2H 17O resonances corresponds to relative magnitude respective couplings. 17O−15N REDOR curves glycine·2HCl; fits initial buildup (ΔS/S < 0.2) yield effective in agreement with (±20%)...
We present an approach to computing multiplets for core spectroscopies, whereby the crystal field is constructed explicitly from positions and charges of surrounding atoms. The simplicity input allows consideration fields any symmetry in particular facilitates study spectroscopic effects arising low-symmetry environments. interplay between polarization directions can also be conveniently investigated. determination proceeds a Dirac density functional atomic calculation, followed by exact...
We present a detailed study of the ferromagnetic/ferroelectric heterostructure ${\mathrm{La}}_{0.7}{\mathrm{Sr}}_{0.3}{\mathrm{MnO}}_{3}/{[\mathrm{Pb}({\mathrm{Mg}}_{1/3}{\mathrm{Nb}}_{2/3}){\mathrm{O}}_{3}]}_{0.68}\ensuremath{-}{[{\mathrm{PbTiO}}_{3}]}_{0.32}$ (011), where reversible electrical switching induces 10 K shift magnetic Curie temperature ${\mathrm{T}}_{C}$. Two distinct polarization configurations can be set in ferroelectric substrate and are stable at remanence; reciprocal...
Toxic proteinaceous deposits and alterations in excitability activity levels characterize vulnerable neuronal populations neurodegenerative diseases. Using vivo two-photon imaging behaving spinocerebellar ataxia type 1 (Sca1) mice, wherein Purkinje neurons (PNs) degenerate, we identify an inhibitory circuit element (molecular layer interneurons [MLINs]) that becomes prematurely hyperexcitable, compromising sensorimotor signals the cerebellum at early stages. Mutant MLINs express abnormally...
Metacestodes of the cestode Echinococcus multilocularis are causative agents alveolar echinococcosis, a rare and neglected, life-threatening, zoonotic disease. To study these metacestodes in vitro, model system using culture medium conditioned by rat hepatoma cells is available. A key question is, how parasite interacts with host particular, which host-derived compounds taken up. In this study, we focus on uptake proteins. Studies artificially labelled proteins suggest that may occur...
Neutron diffraction and inelastic spectroscopy is used to characterize the magnetic Hamiltonian of SrHo2O4 SrDy2O4. Through a detailed computation crystal-field levels we find site- dependent anisotropic single-ion magnetism in both materials measurements show presence strong one-dimensional spin correlations. Our indicate that competing interactions zig-zag chain, combined with frustrated interchain interactions, play crucial role stabilizing spin-liquid type correlations this series.
We report the first empirical demonstration that resonant inelastic x-ray scattering (RIXS) is sensitive to \emph{collective} magnetic excitations in $S=1$ systems by probing Ni $L_3$-edge of La$_{2-x}$Sr$_x$NiO$_4$ ($x = 0, 0.33, 0.45$). The excitation peak asymmetric, indicating presence single and multi spin-flip excitations. As hole doping level increased, zone boundary magnon energy suppressed at a much larger rate than doped cuprates. Based on analysis orbital charge observed RIXS, we...
The positive-sense, single-stranded RNA alphaviruses pose a potential epidemic threat. Understanding the complex interactions between viral and host cell proteins is crucial for elucidating mechanisms underlying successful virus replication strategies developing specific antiviral interventions. Here we present first comprehensive protein-protein interaction map of Semliki Forest Virus (SFV), mosquito-borne member alphaviruses, proteins. Among many identified cellular interactors SFV...
Circulating extracellular vesicles (cEV) are released by many kinds of cells and play an important role in cellular communication, signaling, inflammation modulation, coagulation, tumor growth. cEV growing interest, not only as biomarkers, but also potential treatment targets. However, very little is known about the effect transporting biological samples from clinical ward to diagnostic laboratory, notably on protein composition. Pneumatic tube systems (PTS) human carriers (C) both routinely...
Suppression of premature termination codons (PTCs) by translational readthrough is a promising strategy to treat wide variety severe genetic diseases caused nonsense mutations. Here, we present two potent promoters—NVS1.1 and NVS2.1—that restore substantial levels functional full-length CFTR IDUA proteins in disease models for cystic fibrosis Hurler syndrome, respectively. In contrast other promoters that affect stop codon decoding, the NVS compounds stimulate PTC suppression triggering...
Antifibrotic therapy with nintedanib is the clinical mainstay in treatment of progressive fibrosing interstitial lung disease (ILD). High-dimensional medical image analysis, known as radiomics, provides quantitative insights into organ-scale pathophysiology, generating digital fingerprints. Here, we performed an integrative analysis radiomic and proteomic profiles (radioproteomics) to assess whether changes signatures can stratify degree antifibrotic response (experimental) ILD. Unsupervised...
Leucinostatins are antimicrobial peptides with a broad range of activities against infectious agents as well mammalian cells. The leucinostatin-derivative peptide ZHAWOC_6027 (peptide 6027) was tested in vitro and vivo for activity the intracellular apicomplexan parasite Toxoplasma gondii. While highly efficacious (EC50 = 2 nM), subcutaneous application 6027 (3 mg/kg/day 5 days) mice experimentally infected T. gondii oocysts exacerbated infection, caused mild clinical signs elevated cerebral...
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. The prognosis for patients with high-grade and metastatic disease still very poor, survivors are burdened long-lasting side effects. Therefore, more effective less toxic therapies needed. Surface proteins ideal targets antibody-based therapies, like bispecific antibodies, antibody-drug conjugates, or chimeric antigen receptor (CAR) T-cells. Specific surface RMS scarce. Here, we performed a surfaceome profiling based...
Weak hydrogen bonds are increasingly hypothesized to play key roles in a wide range of chemistry from catalysis gelation polymer structure. Here, 15N/13C spin-echo magic-angle spinning (MAS) solid-state nuclear magnetic resonance (NMR) experiments applied "view" intermolecular CH···N bonding two selectively labeled organic compounds, 4-[15N] cyano-4'-[13C2] ethynylbiphenyl (1) and [15N3,13C6]-2,4,6-triethynyl-1,3,5-triazine (2). The synthesis 2-15N3,13C6 is reported here for the first time...
Abstract Heterogenous nuclear ribonucleoproteins (hnRNPs) are abundant proteins implicated in various steps of RNA processing that assemble on into larger complexes termed 40S hnRNP particles. Despite their initial discovery 55 years ago, our understanding these intriguing macromolecular assemblies remains limited. Here, we report the biochemical purification native particles and determination complete protein composition by label-free quantitative mass spectrometry, identifying A-group...
Nonsense-mediated mRNA decay (NMD) is a eukaryotic RNA pathway with roles in cellular stress responses, differentiation, and viral defense. It functions both quality control post-transcriptional regulation of gene expression. NMD has also emerged as modulator cancer progression, although available evidence supports tumor suppressor pro-tumorigenic role, depending on the model. To further investigate role cancer, we knocked out factor SMG7 HT1080 human fibrosarcoma cell line, resulting...
is an apicomplexan parasite causing persistent diarrhea in humans and animals. Issuing from target-based drug development, calcium-dependent protein kinase 1 inhibitors, collectively named bumped inhibitors (BKIs), with excellent efficacies vitro vivo have been generated. Some BKIs including BKI-1748 share a core structure similarities to the first-generation antiprotozoal quinine, which known exert notorious side effects. Unlike rapidly interfered
the apicomplexan parasite Neospora caninum causes important reproductive problems in farm animals, most notably cattle. After infection via oocysts or tissue cysts, rapidly dividing tachyzoites infect various tissues and organs, immunocompetent hosts, they differentiate into slowly bradyzoites, which form cysts constitute a resting stage persisting within infected tissues. Bumped kinase inhibitors (BKIs) of calcium dependent protein 1 are promising drug candidates for treatment infections....
Alveolar echinococcosis (AE) is caused by infection with the fox tapeworm E. multilocularis. The disease affects humans, dogs, captive monkeys, and other mammals, it metacestode stage of parasite growing invasively in liver. current drug treatment based on non-parasiticidal benzimidazoles. Thus, they are only limitedly curative can cause severe side effects. Therefore, novel improved options for AE needed. Mefloquine (MEF), an antimalarial agent, was previously shown to be effective against...
The larval stage of the cestode Echinococcus multilocularis is causative agent alveolar echinococcosis. To investigate biology these stages and to test novel compounds, metacestode cultures represent a suitable in vitro model system. These metacestodes are vesicles surrounded by an envelope formed vesicle tissue (VT), which laminated germinal layer, filled with fluid (VF). We analyzed proteome VF VT liquid chromatography tandem mass spectrometry (LC-MS/MS) identified total 2,954 parasite...