A5027184769- Hepatitis C virus research
- Hepatitis B Virus Studies
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Advanced biosensing and bioanalysis techniques
- Proteins in Food Systems
- Metal complexes synthesis and properties
- Celiac Disease Research and Management
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Nutritional Studies and Diet
- Mitochondrial Function and Pathology
- Liver Disease Diagnosis and Treatment
- Diabetes and associated disorders
- Metal-Organic Frameworks: Synthesis and Applications
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Amphibian and Reptile Biology
- Adipose Tissue and Metabolism
- Geology and Paleoclimatology Research
- Analytical Chemistry and Sensors
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2025
Wuhan Polytechnic University
2023-2025
Institute of Genetics and Developmental Biology
2010-2024
Chinese Academy of Sciences
2011-2024
Bohai University
2014-2024
Beijing University of Chinese Medicine
2021-2024
Ningxia Medical University
2010-2024
Northeast Agricultural University
2009-2024
Inner Mongolia University
2024
Guangxi Medical University
2009-2024
N6-methyladenosine (m6A) RNA methylation is the most abundant modification on mRNAs and plays important roles in various biological processes. The formation of m6A catalyzed by a methyltransferase complex including methyltransferase-like 3 (METTL3) as key factor. However, vivo functions METTL3 mammalian development remain unclear. Here, we show that specific inactivation Mettl3 mouse nervous system causes severe developmental defects brain. conditional knockout (cKO) mice manifest cerebellar...
Human epidermal growth factor receptor 2 (HER2) expression in breast cancer is associated with an aggressive phenotype and poor prognosis, making it appealing therapeutic target. Trastuzumab, HER2 antibody-based inhibitor, currently the leading targeted treatment for HER2(+)-breast cancers. Unfortunately, many patients inevitably develop resistance to therapy, highlighting need alternative options. In this study, we used a novel, cell-based selection approach isolating 'cell-type specific',...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spreads worldwide and leads to an unprecedented medical burden lives lost. Neutralizing antibodies provide efficient blockade for viral infection are a promising category of biological therapies. Here, using SARS-CoV-2 spike receptor-binding domain (RBD) as bait, we generate panel humanized single (sdAbs) from synthetic library. These sdAbs reveal binding kinetics with the equilibrium dissociation constant ( K D )...
RNA aptamers represent an emerging class of pharmaceuticals with great potential for targeted cancer diagnostics and therapy. Several that bind cell-surface antigens high affinity specificity have been described. However, their clinical has yet to be realized. A significant obstacle the adoption is cost manufacturing long sequences through chemical synthesis. Therapeutic are often truncated postselection by using a trial-and-error process, which time consuming inefficient. Here, we used...
Background The broad applicability of RNA aptamers as cell-specific delivery tools for therapeutic reagents depends on the ability to identify aptamer sequences that selectively access cytoplasm distinct cell types. Towards this end, we have developed a novel approach combines cell-based selection method (cell-internalization SELEX) with high-throughput sequencing (HTS) and bioinformatics analyses rapidly cell-specific, internalization-competent aptamers. Methodology/Principal Findings We...
Transcriptional gene silencing (TGS) can serve as an innate immunity against invading DNA viruses throughout Eukaryotes. Geminivirus code for TrAP protein to suppress the TGS pathway. Here, we identified Arabidopsis H3K9me2 histone methyltransferase, Su(var)3-9 homolog 4/Kryptonite (SUVH4/KYP), a bona fide cellular target of TrAP. interacts with catalytic domain KYP and inhibits its activity in vitro. elicits developmental anomalies phenocopying several mutants, reduces repressive mark CHH...
Cell-targeted therapies (smart drugs), which selectively control cancer cell progression with limited toxicity to normal cells, have been developed effectively treat some cancers. However, many cancers such as metastatic prostate (PC) yet be treated current smart drug technology. Here, we describe the thorough preclinical characterization of an RNA aptamer (A9g) that functions a for PC by inhibiting enzymatic activity prostate-specific membrane antigen (PSMA). Treatment cells A9g results in...
Hepatocellular carcinoma (HCC) and liver cirrhosis are associated with high mortality worldwide. Currently, alpha-fetoprotein (AFP) is used as a standard serum marker for the detection of HCC, but its sensitivity specificity unsatisfactory, optimal diagnostic markers lacking. We previously reported that growth differentiation factor 15 (GDF15) was significantly induced in HCV-infected hepatocytes. This study aimed to investigate GDF15 expression correlation hepatitis virus-related diseases....
Hydrogen sulfide (H2S) contributes to human health and prolongs the storage time of postharvest fruits vegetables. At same time, H2S can cause a negative impact on some foodstuffs beverages, so an efficient probe detect is needed. Herein, fluorescent turn-on responding SPy-DNs for detection has been designed synthesized. exhibited red emission (608 nm), large Stokes shift (111 limit nanomolar level (356 nM) in dimethylformamide/phosphate-buffered saline (DMF/PBS) (1:1, v/v, 10 mM, pH 7.4)...
Abstract The wide transmission and host adaptation of SARS-CoV-2 have led to the rapid accumulation mutations, posing significant challenges effectiveness vaccines therapeutic antibodies. Although several neutralizing antibodies were authorized for emergency clinical use, convalescent patients derived natural are vulnerable Spike mutation. Here, we describe screen a panel receptor-binding domain (RBD) targeted nanobodies (Nbs) from synthetic library design biparatopic Nb, named Nb1–Nb2, with...
Sterol regulatory element binding proteins (SREBPs) are master regulators of hepatic lipid homeostasis.Aberrant expression SREBPs frequently leads to metabolism dysregulation.Long non-coding RNAs (lncRNAs) have been identified with diverse biological functions, but the effects lncRNAs on rarely reported.Here, we a novel human specific lncRNA, lncHR1, as negative regulator SREBP-1c expression.Overexpression lncHR1 inhibited and fatty acid synthase (FAS) then repressed oleic acid-induced cell...
The tomato encode four functional DCL families, of which DCL2 is poorly studied. Here, we generated loss-of-function mutants for a gene, dcl2b, and identified its major role in defending against mosaic virus relation to both natural manual infections. Genome-wide small RNA expression profiling revealed that DCL2b was required the processing 22-nt RNAs, including few species miRNAs. Interestingly, these DCL2b-dependent miRNAs functioned similarly DCL1-produced Arabidopsis could serve as...
// Mengzhu Chen 1 , Xiuying Liu 2 Jie Du 3 Xiu-Jie Wang Lixin Xia State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Department Biochemistry and Molecular Biology, Health Science Center School Medicine, Guangdong 518060, China Genetic Network Collaborative Innovation Genetics Development, Institute Developmental Chinese Academy Sciences, Beijing 100101, Anzhen Hospital, Capital Medical Innovative Cardiovascular Disorders, The Remodeling-Related Diseases, Ministry...
Abstract Aging is an independent risk factor for vascular diseases. Perivascular adipose tissue (PVAT), active component of the vasculature, contributes to dysfunction during aging. Identification underlying cell types and their changes aging may provide meaningful insights regarding clinical relevance aging‐related Here, we take advantage single‐cell RNA sequence characterize resident stromal cells in PVAT (PVASCs) identified different clusters between young aged PVASCs. Bioinformatics...