A5101499495- Hepatitis C virus research
- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Long-Term Effects of COVID-19
- Monoclonal and Polyclonal Antibodies Research
- Muscle Physiology and Disorders
- Cytokine Signaling Pathways and Interactions
- Liver Disease Diagnosis and Treatment
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- COVID-19 Impact on Reproduction
- Barrier Structure and Function Studies
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- GDF15 and Related Biomarkers
- Sepsis Diagnosis and Treatment
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2025
Capital Medical University
2017-2024
Harbin Medical University
2008-2024
First Affiliated Hospital of Harbin Medical University
2020-2024
Jilin Medical University
2022-2024
Shaanxi Provincial People's Hospital
2024
Shanxi Medical University
2024
Xiamen Chang Gung Hospital
2024
University of Chinese Academy of Sciences
2019-2024
Beijing Sanbo Brain Hospital
2022-2023
A tight junction (TJ) protein, claudin-1 (CLDN1), was identified recently as a key factor for hepatitis C virus (HCV) entry. Here, we show that another TJ occludin, is also required HCV Mutational study of CLDN1 revealed its junctional distribution plays an important role in mediating viral Together, these data support the model which enters liver cells from TJ. Interestingly, infection Huh-7 hepatoma downregulated expression and preventing superinfection. The altered protein may contribute...
Abstract The efficacy of checkpoint immunotherapy to non-small cell lung cancer (NSCLC) largely depends on the tumor microenvironment (TME). Here, we demonstrate that CCL7 facilitates anti-PD-1 therapy for Kras LSL−G12D/+ Tp53 fl/fl (KP) and Lkb1 (KL) NSCLC mouse models by recruiting conventional DC 1 (cDC1) into TME promote T expansion. exhibits high expression in tissues is positively correlated with infiltration cDC1 overall survival patients. deficiency impairs subsequent expansion CD8 +...
Hepatitis C virus (HCV) is a major human pathogen that causes serious illness, including acute and chronic hepatitis, cirrhosis, hepatocellular carcinoma. Using mass spectrometry–based proteomics approach, we have identified 175 proteins from cell culture supernatant fraction containing the HCV genotype 2a (JFH1) virus, among which fatty acid synthase (FASN), multifunctional enzyme catalyzing de novo synthesis of acids, was confirmed to be highly enriched. Subsequent studies showed FASN...
Sixty-two new Japanese encephalitis virus (JEV) isolates were obtained from mosquitoes, biting midges, human cerebrospinal fluid and blood samples in China during 2002-2005. The E prM genes sequenced phylogenetic analyses performed with 38 JEV other 36 strains countries. Phylogenetic trees based on the gene sequences similar. results indicate that: (i) recent are divided into two genotypes, genotype 1 3; (ii) grouped same clusters within genotypes (iii) have been isolated since 1979, whilst...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spreads worldwide and leads to an unprecedented medical burden lives lost. Neutralizing antibodies provide efficient blockade for viral infection are a promising category of biological therapies. Here, using SARS-CoV-2 spike receptor-binding domain (RBD) as bait, we generate panel humanized single (sdAbs) from synthetic library. These sdAbs reveal binding kinetics with the equilibrium dissociation constant ( K D )...
Hepatitis C virus (HCV) is able to induce autophagy via endoplasmic reticulum (ER) stress, but the exact molecular signaling pathway not well understood. We found that activity of mechanistic target rapamycin complex 1 (MTORC1) was inhibited in Huh7 cells either harboring HCV-N (genotype 1b) full-genomic replicon or infected with JFH1 2a) virus, which led activation UNC-51-like kinase (ULK1) and thus autophagy. then analyzed upstream MTORC1, both protein kinase, AMP-activated, α (PRKAA,...
Hepatocellular carcinoma (HCC) and liver cirrhosis are associated with high mortality worldwide. Currently, alpha-fetoprotein (AFP) is used as a standard serum marker for the detection of HCC, but its sensitivity specificity unsatisfactory, optimal diagnostic markers lacking. We previously reported that growth differentiation factor 15 (GDF15) was significantly induced in HCV-infected hepatocytes. This study aimed to investigate GDF15 expression correlation hepatitis virus-related diseases....
To combat the coronavirus disease 2019 (COVID-19) pandemic, nonpharmaceutical interventions (NPIs) were implemented worldwide, which impacted a broad spectrum of acute respiratory infections (ARIs).Etiologically diagnostic data from 142 559 cases with ARIs, who tested for 8 viral pathogens (influenza virus [IFV], syncytial [RSV], human parainfluenza [HPIV], adenovirus [HAdV], metapneumovirus [HMPV], [HCoV], bocavirus [HBoV], and rhinovirus [HRV]) between 2012 2021, analyzed to assess changes...
Abstract The wide transmission and host adaptation of SARS-CoV-2 have led to the rapid accumulation mutations, posing significant challenges effectiveness vaccines therapeutic antibodies. Although several neutralizing antibodies were authorized for emergency clinical use, convalescent patients derived natural are vulnerable Spike mutation. Here, we describe screen a panel receptor-binding domain (RBD) targeted nanobodies (Nbs) from synthetic library design biparatopic Nb, named Nb1–Nb2, with...
Abstract The cytokines of transforming growth factor β (TGF-β) and its superfamily members are potent regulators tumorigenesis multiple cellular events. Myostatin is a member TGF-β plays negative role in the control cell proliferation differentiation. We now show that myostatin rapidly activated extracellular signal–regulated kinase 1/2 (Erk1/2) cascade C2C12 myoblasts. A more remarkable Erk1/2 activation stimulated by was observed differentiating cells than proliferating cells. results also...
Claudin-1 (CLDN1), a tight junction (TJ) protein, has recently been identified as an entry co-receptor for hepatitis C virus (HCV). Ectopic expression of CLDN1 rendered several non-hepatic cell lines permissive to HCV infection. However, little is known about the mechanism by which mediates entry. It believed that additional receptor(s) required because ectopic in both HeLa and NIH3T3 cells failed confer susceptibility viral Here we found was co-immunoprecipitated with envelope proteins when...
Sterol regulatory element binding proteins (SREBPs) are master regulators of hepatic lipid homeostasis.Aberrant expression SREBPs frequently leads to metabolism dysregulation.Long non-coding RNAs (lncRNAs) have been identified with diverse biological functions, but the effects lncRNAs on rarely reported.Here, we a novel human specific lncRNA, lncHR1, as negative regulator SREBP-1c expression.Overexpression lncHR1 inhibited and fatty acid synthase (FAS) then repressed oleic acid-induced cell...
Zika virus (ZIKV) is a unique flavivirus with high tropism to the testes. ZIKV can persist in human semen for months and cause testicular damage male mice. However, mechanisms through which enters testes remain unclear. In this study, we revealed that matrix metalloproteinase 9 (MMP9) was upregulated by infection cell culture A129 Furthermore, using an vitro Sertoli barrier model MMP9-/- mice, found directly affected permeability of blood-testis (BTB), knockout or inhibition MMP9 reduced...
During its global epidemic, Zika virus (ZIKV) attracted widespread attention due to link with various severe neurological symptoms. and potential harm male fertility. However, the understanding of how ZIKV invades persists in reproductive system is limited lack immunocompetent small animal models. In this study, murine models were generated by using anti-IFNAR antibody blocked C57BL/6 mice human STAT2 (hSTAT2) knock (KI) mice. After infection, viral RNA could persist testes even after...
ABSTRACT Hepatitis C virus (HCV) infection is a major cause of severe liver disease. Interferon (IFN)/ribavirin treatment remains the standard therapeutic regimen for HCV in most countries. IFN-stimulated genes are believed to contribute antiviral effects. However, emerging evidence suggests that microRNAs (miRNAs), class noncoding small RNAs, involved control viral infection. Here, we systematically profiled hepatocyte expression set 750 miRNAs response alpha interferon (IFN-α) and...
The association between triglyceride(TG) levels and the risk of diabetes mellitus (DM) continues to be a subject considerable interest debate within scientific community. To date, there has been lack studies specifically examining this relationship Chinese population. This study seeks elucidate correlation TG incidence DM among demographic. constitutes secondary analysis retrospective cohort investigation comprising 202,888 participants who were free at baseline subsequently followed from...
Hepatitis C virus (HCV) entry is a complicated process that requires multiple host factors, such as CD81, scavenger receptor BI, claudin-1 (CLDN1), and occludin. The interaction of cellular factors represents promising target for novel anti-HCV drug development. In this study, we sought to identify peptide inhibitors HCV by screening library overlapping peptides covering the four above-mentioned factors. An 18-amino acid (designated CL58) was derived from CLDN1 intracellular first...
Zika virus (ZIKV) has caused major concern globally due to its rapid dissemination and close association with microcephaly in children Gullian-Barr syndrome adults. In this study, we identified a patient returned from Cambodia who experienced high fever, chill myalgia. Lab tests discovered sign of severe liver injury including significantly elevated serum transaminases' level, decreased albumin markedly increased levels lactic dehydrogenase, alpha-hydroxybutyric dehydrogenase creatine kinase...
Pattern recognition receptors sense pathogen components and initiate the host antiviral innate immune response, such as inducing interferons (IFNs). Long non-coding RNAs (lncRNAs) are emerging regulators of multiple biological processes. However, their role in especially through regulating human immune, is largely unexplored. Here we characterized that lncATV, a specific lncRNA, was up-regulated upon type I/III IFN stimulations virus infection. LncATV cytoplasmic localized relatively high...
Both the endothelial transporter Mfsd2a and docosahexaenoic acid uptake are disrupted in Zika virus–induced microcephaly models.
In this study, we aimed to determine whether continuous renal replacement therapy (CRRT) with oXiris filter may alleviate cytokine release syndrome (CRS) in non-AKI patients severe and critical coronavirus disease 2019 (COVID-19). A total of 17 COVID-19 treated between February 14 March 26, 2020 were included randomly divided into intervention group control according the random number table. Patients immediately received CRRT plus conventional treatment, while those only treatment....