A5056125661- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Metabolism and Genetic Disorders
- Ginseng Biological Effects and Applications
- RNA modifications and cancer
- interferon and immune responses
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- NF-κB Signaling Pathways
- Complement system in diseases
- Medical Research and Treatments
- RNA regulation and disease
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Epigenetics and DNA Methylation
- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- Influenza Virus Research Studies
- Respiratory and Cough-Related Research
- Gut microbiota and health
- MicroRNA in disease regulation
- Natural product bioactivities and synthesis
- Effects of Radiation Exposure
- IL-33, ST2, and ILC Pathways
Women's Hospital, School of Medicine, Zhejiang University
2020-2025
Beijing University of Chinese Medicine
2025
Henan University of Engineering
2024
Henan Polytechnic University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2023
National Health and Family Planning Commission
2023
Yantai University
2020-2023
Academy of Medical Sciences
2020
First Affiliated Hospital of Zhengzhou University
2020
Weatherford College
2020
<h3>Importance</h3> Convalescent plasma is a potential therapeutic option for patients with coronavirus disease 2019 (COVID-19), but further data from randomized clinical trials are needed. <h3>Objective</h3> To evaluate the efficacy and adverse effects of convalescent therapy COVID-19. <h3>Design, Setting, Participants</h3> Open-label, multicenter, trial performed in 7 medical centers Wuhan, China, February 14, 2020, to April 1, final follow-up 28, 2020. The included 103 participants...
The pandemic of COVID-19 has posed an unprecedented threat to global public health. However, the interplay between viral pathogen COVID-19, SARS-CoV-2, and host innate immunity is poorly understood. Here we show that SARS-CoV-2 induces overt but delayed type-I interferon (IFN) responses. By screening 23 proteins, find NSP1, NSP3, NSP12, NSP13, NSP14, ORF3, ORF6 M protein inhibit Sendai virus-induced IFN-β promoter activation, whereas NSP2 S exert opposite effects. Further analyses suggest...
Abstract SARS-CoV-2 is the pathogenic agent of COVID-19, which has evolved into a global pandemic. Compared with some other respiratory RNA viruses, poor inducer type I interferon (IFN). Here, we report that nsp12, viral RNA-dependent polymerase (RdRp), suppresses host antiviral responses. nsp12 attenuated Sendai virus (SeV)- or poly(I:C)-induced IFN-β promoter activation in dose-dependent manner. It also inhibited IFN triggered by RIG-I, MDA5, MAVS, and IRF3 overexpression. Nsp12 did not...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spreads worldwide and leads to an unprecedented medical burden lives lost. Neutralizing antibodies provide efficient blockade for viral infection are a promising category of biological therapies. Here, using SARS-CoV-2 spike receptor-binding domain (RBD) as bait, we generate panel humanized single (sdAbs) from synthetic library. These sdAbs reveal binding kinetics with the equilibrium dissociation constant ( K D )...
Abstract Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) caused the Corona Virus Disease 2019 (COVID-19) cases in China has become a public health emergency of international concern (PHEIC). Based on angiotensin converting enzyme 2 (ACE2) as cell entry receptor SARS-CoV, we used hACE2 transgenic mice infected with SARS-CoV-2 to study pathogenicity virus. Weight loss and virus replication lung were observed SARS-CoV-2. The typical histopathology was interstitial pneumonia infiltration...
COVID-19 pandemic has infected millions of people with mortality exceeding >1 million. There is an urgent need to find therapeutic agents that can help clear the virus prevent severe disease and death. Identifying effective safer drugs provide more options treat infections either alone or in combination. Here, we performed a high throughput screening approximately 1,700 US FDA-approved compounds identify novel effectively inhibit replication coronaviruses including SARS-CoV-2. Our two-step...
Abstract The membrane-associated RING-CH (MARCH) proteins are E3 ligases that regulate the stability of various cellular membrane proteins. MARCH8 has been reported to inhibit infection HIV-1 and a few other viruses, thus plays an important role in host antiviral defense. However, spectrum underlying mechanisms incompletely defined. Here, we demonstrate profoundly inhibits influenza A virus (IAV) replication both vitro mice. Mechanistically, suppresses IAV release through redirecting viral...
Rationale: Alteration of human respiratory microbiota had been observed in coronavirus disease (COVID-19). How the is associated with prognosis COVID-19 unclear. Objectives: To characterize feature and dynamics its associations clinical features patients COVID-19. Methods: We conducted metatranscriptome sequencing on 588 longitudinal oropharyngeal swab specimens collected from 192 (including 39 deceased patients) 95 healthy controls same geographic area. Meanwhile, concentration 27 cytokines...
SARS-CoV-2 has become a global threat to public health. Infected individuals can be asymptomatic or develop mild severe symptoms, including pneumonia, respiratory distress, and death. This wide spectrum of clinical presentations infection is believed in part due the polymorphisms key genetic factors population. In this study, we report that interferon-induced antiviral factor IFITM3 inhibits by preventing spike-protein-mediated virus entry cell-to-cell fusion. Analysis Chinese COVID-19...
Cancer patients treated with current chemotherapeutic and targeted therapies frequently achieve partial remission, which ultimately relapse more aggressive, drug-resistant tumor phenotypes. To a certain extent, drug-tolerant persister (DTP) cells are responsible for residual tumors after systemic anticancer therapy the onset of acquired drug resistance. Therefore, novel targeting DTP to prevent resistance recurrence urgently needed. We aimed investigate traits key vulnerabilities ovarian...
Background: Acute lung injury (ALI) is a major cause of death in patients with various viral pneumonias. Our team previously identified four volatile compounds from aromatic Chinese medicines. Based on molecular compatibility theory, we defined their combination as (AC), though its therapeutic effects and underlying mechanisms remain unclear. Methods: This study used influenza A virus (IAV) A/PR/8/34 to construct cell mouse models ALI explore AC’s protective against infection. The effect AC...
Polarity defects are frequently involved in liver diseases, such as chronic hepatitis and hepatocellular carcinoma (HCC). It was reported that vacuolar protein sorting 33B (Vps33b) plays critical roles the maintenance of hepatocyte polarity; however, functional mechanisms Vps33b HCC occurrence progression remain unknown. First all, we showed is down‐regulated human mouse cancer samples, low expression levels correlate with poor prognosis many patients. Liver‐specific deficiency induces...
<b><i>Background:</i></b> Neuromyelitis optica (NMO) and multiple sclerosis (MS) are two autoimmune inflammatory demyelinating diseases in the central nervous system. Interleukin (IL)-6 IL-17 may play important roles pathogenesis of these diseases. High-mobility group box protein 1 (HMGB1) can activate nuclear factor <!-- NICHT DEFINIERT -Kleines Kapitaelchen K --> light chain enhancer activated B cells release cytokines such as IL-6 IL-17. However, whether...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread across more than 200 countries and regions, leading to an unprecedented medical burden live lost. SARS-CoV-2 specific antivirals or prophylactic vaccines are not available. Neutralizing antibodies provide efficient blockade for viral infection a promising category of biological therapies. Using spike RBD as bait, we have discovered panel humanized single domain (sdAbs). These sdAbs revealed binding kinetics with...
Rationale: Respiratory syncytial virus (RSV) is the leading cause of childhood respiratory infections worldwide; however, no vaccine available, and treatment options are limited. Identification host factors pivotal to viral replication may inform development novel therapies, prophylaxes, or diagnoses.Objectives: To identify involved in RSV evaluate their potential for disease management.Methods: A gain-of-function screening was performed on basis a genome-wide human complementary DNA library...
To identify the association between kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized COVID-19 patients LOTUS China trial. Among collected 578 paired throat swab (TS) anal (AS) samples, RNA detected 193 (33.4%) TS 121 (20.9%) AS. A higher found than that AS, with means 1.0 × 106 2.3 105 copies/ml, respectively. In non-survivors, AS earlier survivors (median 14 days vs 19 days, P...
Small extracellular vesicles (sEVs) and large (lEVs), play vital roles in intercellular communication. We optimized a method that extracts EVs from epithelial ovarian cancer (EOC) tissues for the purpose of investigating whether cryopreservation EOC affects phenotypes, contents, biological functions extracted EVs. EV morphology, number size distribution EVs, EV-related markers were analyzed. Storage lysates at -80°C decreased lEV yield increased sEV yield, whereas storage both yields;...
The coronavirus disease 2019 (COVID-19) epidemic has given a warning that it is important to explore the rapid detection of severe acute respiratory syndrome 2 (SARS-CoV-2) in clinical specimens or environmental samples for public health strategies and future variants. surface-enhanced Raman spectroscopy (SERS) technique was demonstrated achieve this goal. However, consistency signals originating from poor compatibility virions with SERS hotspots remains key scientific challenge practical...
Vascular protein sorting-associated 33B (VPS33B) plays important roles in hepatic polarity, which directly maintains the functional structure of liver. It has reported that VPS33B close association with arthrogryposis, renal dysfunction and cholestasis (ARC) syndrome. Unfortunately, no further studies were conducted to reveal role Vps33b homeostasis bile acids. In current study, Vps33b-depleted male mice used investigate metabolomics lipidomics profiles deficiency based on...
Pyxinol, the main metabolite of 20S-protopanaxadiol in human liver, was chosen as a novel skeleton for development anti-inflammatory agents. Pyxinol derivatives modified at C-3, C-12, or C-25 and selected stereoisomers were designed, prepared, investigated vitro activities. Structure–activity relationship (SAR), focused on skeleton, analyzed based their ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) synthesis. The preliminary SAR results signified that biological...