A5021307310- Ruminant Nutrition and Digestive Physiology
- Gut microbiota and health
- Reproductive Physiology in Livestock
- Clostridium difficile and Clostridium perfringens research
- Craniofacial Disorders and Treatments
- Immune Response and Inflammation
- Mycobacterium research and diagnosis
- Genetic and phenotypic traits in livestock
- Neonatal Respiratory Health Research
- Pneumonia and Respiratory Infections
- Facial Rejuvenation and Surgery Techniques
- Immune cells in cancer
- Turfgrass Adaptation and Management
- Respiratory viral infections research
- Microbial infections and disease research
- Heavy metals in environment
- Muscle metabolism and nutrition
- Viral gastroenteritis research and epidemiology
- Facial Trauma and Fracture Management
- Plant Surface Properties and Treatments
- Orthodontics and Dentofacial Orthopedics
- Plant and fungal interactions
- Hippo pathway signaling and YAP/TAZ
- Probiotics and Fermented Foods
- Bioenergy crop production and management
Zhejiang University of Science and Technology
2023-2024
Vision Medicals (China)
2022-2024
Zhejiang University
2015-2024
Hunan Academy of Agricultural Sciences
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2023
Beijing Shijingshan Hospital
2023
Jiangxi Agricultural University
2018-2022
Patients First
2022
Academy of Medical Sciences
2022
Beijing Municipal Education Commission
2022
The gut microbiota impacts on a range of host biological processes, and the imbalances in its composition are associated with pathology. Though understanding contribution many factors, e.g. gender, diet age, development has been well established, dynamic changes phylogenetic interaction networks along age remain unclear pigs.Here we applied 16S ribosomal RNA gene sequencing, enterotype-like clustering (Classification microbiome into distinct types) co-occurrence network to explore pig...
Alteration of the lung microbiome has been observed in several respiratory tract diseases. However, most previous studies were based on 16S ribosomal RNA and shotgun metagenome sequencing; viability functional activity microbiome, as well its interaction with host immune systems, have not studied. To characterize active associations response clinical features, we applied metatranscriptome sequencing to bronchoalveolar lavage fluid (BALF) samples from 25 patients chronic obstructive pulmonary...
Diarrhea is a major cause of morbidity and mortality in young calves, resulting considerable economic loss for dairy farms. To determine if some gut microbes might have resistance to dysbiotic process with calf diarrhea by dictating the microbial co-occurrence patterns from birth post-weaning, we examined dynamic development microbiota status using two animal trials, first trial having 14 Holstein calves whose fecal samples were collected 18 times over 78 d 15 post-weaning second 43 daily 8...
The pre-weaning period is crucial for rumen developmental plasticity, which can have a long-term impact on animal performance. Understanding the microbiota during early life important to elucidate its potential role in development. In this study, of 10-day-old Hu lambs fed either milk replacer (B-10), and starter (STA) or supplemented with alfalfa (S-ALF) pre- (d17, 24, 38) post-weaning periods (d45 66) were assessed characterize microbial colonization response fiber intervention. rumens...
The rumen and the hindgut represent two different fermentation organs in herbivorous mammals, with former producing much more methane than latter. objective of this study was to elucidate microbial underpinning such differential outputs between these digestive organs. Methane production measured from 5 adult sheep 15 rabbits, both which were placed open-circuit respiratory chambers fed same diet (alfalfa hay). produced rabbits per unit metabolic body weight, digestible neutral detergent...
Summary Under natural farming, environmental pathogenic microorganisms may invade and affect swine lungs, further resulting in lung lesions. However, few studies on microbiota their potential relationship with lesions were reported. Here, we sampled 20 pigs from a hybrid herd raised under conditions; recorded lung‐lesion phenotype investigated microbial communities by sequencing the V3‐V4 region of 16S rRNA gene for each individual. We found reduced diversity but more biomass severe‐lesion...
This study aims to determine the effects of alfalfa supplementation on pre- and postweaning performance, rumen development, feed transition in starter diet-fed lambs. Six 66 male Hu lambs were slaughtered at age 10 d serve as a control. The other 60 randomly allocated 2 dietary treatments: milk replacer pellets without (STA) or with free-choice chopped (S-ALF). animals offered 300 g/d concentrate mixture had free access after weaning end wk 4 (age 38 d). inclusion S-ALF group tended increase...
Myeloid-derived suppressor cells (MDSCs) are regulatory cell populations that have the ability to suppress effector T responses and promote development of (Tregs). They a heterogeneous population immature myeloid progenitors include monocytic granulocytic subsets. We postulated given rapid expansion post-transplant, these members innate immune system may be important contributors early environment post-transplant. To evaluate kinetics recovery function MDSCs after allogeneic hematopoietic...
Improving feed efficiency is crucial to the animal industry. Residual intake (RFI) now regarded as an index of evaluation and independent growth characteristics. Our study aims explore alterations in performance nutrient digestion Hu sheep with different RFI phenotypes. Sixty-four male (body weight = 24.39 ± 1.12 kg; postnatal days 90 7.9) were selected for study. After period 56 power analysis, samples collected from 14 low (L-RFI group, 0.95) high (H-RFI 0.95). The L-RFI yielded a lower (P...
Abstract Background The current understanding to the mechanism of rumen development is limited. We hypothesized that Hippo signaling pathway controlled proliferation epithelium (RE) during postnatal development. In present study, we firstly tested changes in RE an early growing period from d5 d25, and then expanded time range whole preweaning (d10-38) one week post weaning (d45). An vitro experiment was also carried out verify function cell proliferation. Results lambs expression baculoviral...
The MASS cohort comprises 2000 ICU patients with severe pneumonia, covering community-acquired hospital-acquired and ventilator-associated sourced from 19 hospitals across 10 cities in three provinces. A wide array of samples including bronchoalveolar lavage fluid, sputum, feces, whole blood are longitudinally collected throughout patients' stays. study seeks to uncover the dynamics lung gut microbiomes their associations pneumonia host susceptibility, integrating deep metagenomics...
Combining sweet sorghum (SS) with alfalfa for ensiling has the potential to make a high-quality silage relatively better nutrient balance. However, existing data are insufficient about how changes in ratios of SS different mixtures affect animal performance and meat quality. Therefore, objective this study was determine effect feeding total mixed rations containing silages on growth performance, digestibility, carcass traits quality Karakul sheep. Five were formulated proportions at 100%,...
Rationale: Respiratory syncytial virus (RSV) is the leading cause of childhood respiratory infections worldwide; however, no vaccine available, and treatment options are limited. Identification host factors pivotal to viral replication may inform development novel therapies, prophylaxes, or diagnoses.Objectives: To identify involved in RSV evaluate their potential for disease management.Methods: A gain-of-function screening was performed on basis a genome-wide human complementary DNA library...
Abstract Background Increasing evidence revealed that lung microbiota dysbiosis was associated with pulmonary infection in transplant recipients (LTRs). Pneumocystis jirovecii ( P. ) is an opportunistic fungal pathogen frequently causes lethal pneumonia LTRs. However, the LTRs (PJP) remains unknow. Methods In this prospective observational study, we performed metagenomic next-generation sequencing (mNGS) on 72 bronchoalveolar lavage fluid (BALF) samples from 61 (20 PJP, 22 PJC, 19...
Promoting rumen development is closely related to the health and efficient growth of ruminants. The transcriptional co-activators Yes1-associated protein (YAP1) WW domain-containing transcription regulator 1 (TAZ) are key regulators mammalian epithelium. In present study, we assessed impact YAP1/TAZ on epithelial (RE) cell proliferation using their activator GA-017 (GA) inhibitor verteporfin (VP). We also investigated whether YAP1/TAZ-dependent alteration was involved in RE developmental...
The maternal rumen microbiota can affect the infantile and likely offspring growth, some microbes are heritable associated with host traits. However, little is known about of their role in effect on growth young ruminants. From analyzing ruminal bacteriota from 128 Hu sheep dams 179 lambs, we identified potential bacteria developed random forest prediction models to predict birth weight, weaning preweaning gain ruminants using as predictors. We showed that tended shape offspring. About 4.0%...
Ribulose 5-phosphate (Ru5P) and guanosine 5′-triphosphate (GTP) are two key precursors of riboflavin, whereby Ru5P is also a precursor GTP. 3-epimerase (Rpe) catalyzes the conversion ribulose into xylulose 5-phosphate. Inactivation Rpe can reduce consumption Ru5P, enhancing carbon flux toward riboflavin biosynthesis. Here we investigated effect mutation rpe other related genes on production, physiological metabolic phenotypes in Bacillus subtilis LY (BSLY). Introducing single nucleotide...
A healthy gut is very important for young animal development. The rumen of ruminants expands in size with the colonization microbiota by 2 months age. This process promoted alfalfa intervention. To elucidate mechanism this promotion, we performed transcriptomic analyses using a cohort 23 lambs to evaluate effects starter diets plus on development wall from pre- postweaning period. quantitative PCR were used validate selected genes that differentially expressed transcriptome mapping. We found...
Cadmium (Cd) is an environmental pollutant, widely existing in soil, and can be absorbed accumulated by plants. Hunan Province exhibits the worst cadmium contamination of farmland China. Ruminants possess abundant microbial population rumen, which enables them to tolerate various poisonous To investigate whether rumen microbiota could respond Cd mitigate toxicity Cd-accumulated maize ruminants, 6-month-old cattle were fed with 85.82% (fresh basis) normal whole-plant silage diet (CON, n = 10)...
Ruminants play a vital economic role as livestock, providing high-quality protein for humans. At present, 3D-cultured ruminant abomasum and intestinal organoids have been successfully established to study host pathogen interaction. The rumen is unique digestive organ of ruminants that occupies 70% the volume tract its microbiota can decompose lignocellulose support animal growth. Here we report method culturing epithelial organoids. We found single cells form self-organized 3D structures...
Supplementation of the sheep diet with oats (Avena sativa L.) improves animal growth and meat quality, however effects on intestinal microbes their metabolites was not clear. This study aimed to establish effect dietary oat supplementation rumen colonic microbial abundance explore relationship subsequent changes in digesta metabolites. Twenty Small-tail Han were randomly assigned a containing 30 g/100 g maize straw (Control) or hay (Oat). After 90-days experimental diets, colon collected...