Donor-derived cell-free DNA (dd-cfDNA) has been applied to monitor acute rejection (AR) in kidney and heart transplantation. This study was aimed investigate the application of dd-cfDNA levels diagnosis AR chronic lung allograft dysfunction (CLAD) among transplantation recipients (LTRs).One hundred seventy LTRs were enrolled at First Affiliated Hospital Guangzhou Medical University between 1 June 2015 30 March 2021. Patients divided into 4 groups: stable group, infection group CLAD group....

SARS-CoV-2 infection and the resultant COVID-19 pneumonia cause significant damage to airway lung epithelium. This manifests as mucus hypersecretion, pulmonary inflammation fibrosis, which often lead long-term complications collectively referred long COVID or post-acute sequelae of (PASC). The epithelium, first line defence against respiratory pathogens, depends on basal stem cells (BSCs) for regeneration. Alterations in BSCs are associated with impaired epithelial repair may contribute...

Background: Accurate identification of pathogens is essential for the diagnosis and control infections. We aimed to compare diagnostic performance metagenomic next-generation sequencing (mNGS) conventional detection methods (CDM) in lung transplant recipients (LTRs). Methods: retrospectively analyzed 107 LTRs with suspected infection pulmonary, blood, central nervous system or chest wall between March 2018 November 2020. Bronchoalveolar lavage fluid other body fluids were subject pathogen by...

Surfactant protein D (SP-D) is a lung-specific proposed to predict clinical outcomes in patients with chronic obstructive pulmonary disease (COPD). However, the changes serum SP-D during acute exacerbation (AECOPD) episodes and relationship of overall severity stable COPD (SCOPD) remain unclear.Serum levels were analyzed three groups, including AECOPD (n=40), SCOPD (n=71), controls (n=60). In group, determined at 1, 5, 14, 30 days post-exacerbation. BODE (body mass index, airflow...

Background : Surfactant protein D (SP-D) is a lung-specific proposed to predict clinical outcomes in patients with chronic obstructive pulmonary disease (COPD). However, the changes serum SP-D during acute exacerbation (AECOPD) episodes and relationship of overall severity stable COPD (SCOPD) remain unclear. Methods Serum levels were analyzed three groups, including AECOPD ( n =40), SCOPD =71), controls =60). In group, determined at 1, 5, 14, 30 days post-exacerbation. BODE (body mass index,...

Abstract Background Increasing evidence revealed that lung microbiota dysbiosis was associated with pulmonary infection in transplant recipients (LTRs). Pneumocystis jirovecii ( P. ) is an opportunistic fungal pathogen frequently causes lethal pneumonia LTRs. However, the LTRs (PJP) remains unknow. Methods In this prospective observational study, we performed metagenomic next-generation sequencing (mNGS) on 72 bronchoalveolar lavage fluid (BALF) samples from 61 (20 PJP, 22 PJC, 19...

Background Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and fatal fibrotic disease with unknow etiology. Owing to lack of reliable prognostic biomarkers effective treatment measures, patients IPF usually exhibit poor prognosis. The aim this study establish risk score model for predicting the prognosis based on autophagy-related genes. Methods GSE70866 dataset was obtained from gene expression omnibus (GEO) database. genes were collected Molecular Signatures Database...

Chronic obstructive pulmonary disease (COPD) is associated not only with airway inflammation characterized by mucin hypersecretion but also systemic inflammation. Tumor necrosis factor alpha (TNF-alpha) found to take part in inflammation, and ErbB3 plays an important role of COPD. Since TNF-alpha converting enzyme (TACE) involved the activation both ErbB3, we established rat models COPD investigate expressions TACE, explore correlations among respectively.Thirty Wistar male rats were...

Background Quadriceps muscle dysfunction is well confirmed in chronic obstructive pulmonary disease (COPD) and reported to be related a higher risk of mortality. Factors contributing quadriceps have been postulated, while not one alone could fully explain it there are few reports on China. This study was aimed investigate the severity patients with COPD, compare function COPD healthy elderly. Methods strength endurance capabilities were investigated 71 60 age-matched controls; predicted...

Fibroblast activation plays an important role in the occurrence and development of idiopathic pulmonary fibrosis (IPF), which is a progressive, incurable, fibrotic lung disease. However, underlying mechanism fibroblast IPF remains elusive. Here, we showed that expression levels STX11 SNAP25 were downregulated tissues from patients with mice bleomycin (BLM)-induced as well activated fibroblasts. Upregulation or suppressed TGF-β1-induced human fibroblasts (HLFs) via promoting autophagy. they...

This study was designed to determine the expression of serum and sputum surfactant protein D (SP‑D) in chronic obstructive pulmonary disease (COPD) its association with treatment response. Sixty‑five treatment‑naive patients COPD 26 normal control subjects were recruited study. The concentrations SP‑D measured, associations function modified Medical Research Council dyspnea scale (mMRC) St. George's Respiratory Questionnaire (SGRQ) scores before after three months an inhaled corticosteroid a...

High-throughput next-generation sequencing (HT-NGS) has the potential to detect a large variety of pathogens; however, application HT-NGS in lung transplant (LTx) recipients remains limited. We aimed evaluate value for pathogen detection and diagnosis pulmonary infection during early-stage post-lung transplantation.In this retrospective study, we enrolled 51 LTx who underwent transplantation between January 2020 December 2020. Bronchoalveolar lavage fluid (BALF) samples were collected...

Objective To analyze plasma myostatin levels and investigate their relationship with right ventricular (RV) function in patients cor pulmonale secondary to chronic obstructive pulmonary disease (COPD). Methods The study recruited 81 advanced COPD 40 age-matched controls. were divided into two groups: those without. Echocardiography was used evaluate RV morphology, the value of tricuspid annular plane systolic excursion (TAPSE) less than 16 mm considered dysfunction. Plasma analyzed by...

Infection and rejection are the two most common complications after lung transplantation (LT) associated with increased morbidity mortality. We aimed to examine association between airway microbiota infection in transplant recipients (LTRs). Here, we collected 181 sputum samples (event-free, n = 47; infection, 103; rejection, 31) from 59 LTRs, performed 16S rRNA gene sequencing analyze microbiota. A significantly different was observed among event-free, recipients, including microbial...

Patients with work-related lung disease (WRLD) are at increased risk of death caused by severe tissue damage and fibrosis. This study aimed to assess the clinical outcomes transplantation (LTx) for WRLD compare results LTx between idiopathic pulmonary fibrosis (IPF).This single-center retrospective cohort reviewed data patients who underwent or IPF our hospital January 2015 December 2021. Cumulative survival rates after were estimated using Kaplan-Meier method.The final analysis included 33...

<h3>INTRODUCTION:</h3> Patients with COPD have impaired respiratory muscle strength. Twitch mouth pressure (P_tw,m) in response to magnetic stimulation of the cervical nerve has been suggested clinically reflect inspiratory However, studies on P_tw,m values and their relationship disease severity are limited. Thus, we tested subjects investigated these severity. <h3>METHODS:</h3> We recruited 75 patients 63 age-matched controls. All participants were for P_tw,m,...

Objective: To investigate the relationship between plasma myostatin levels and right ventricle (RV) dysfunction (RVD) in acute exacerbation of chronic obstructive pulmonary disease (AECOPD).Methods: The study recruited 84 patients with AECOPD. Plasma was analyzed tricuspid annular plane systolic excursion (TAPSE) < 16 mm used as main indicator for RVD.Results: were significantly higher 47 RVD than 37 ones without (P 0.005). Multivariate regression analysis revealed that correlated TAPSE...

Cytomegalovirus (CMV) infection is a leading cause of morbidity and mortality after transplantation. This study aimed to investigate CMV seroprevalence, infection, disease in Chinese thoracic organ transplant recipients.The clinical data the patients who underwent lung and/or heart transplantation between January 2015 October 2020 were retrospectively collected from four centers China.A total 308 analyzed. The serostatus was donor positive (D+) recipient negative (R-) 19 (6.17%) patients,...

This study explored the epidemiology, risk factors, and prognosis of invasive fungal disease (IFD) in Chinese lung transplant recipients (LTRs). retrospective cohort included patients who received transplants at four hospitals South China between January 2015 June 2019. The participants were divided into IFD non-IFD (NIFD) groups. final analysis 226 LTRs (83.2% males) aged 55.0 ± 14.2 years old. Eighty-two (36.3%) developed (proven or probable diagnosis). most common pathogens Aspergillus...

Abstract Since the coronavirus disease 2019 (COVID‐19) was identified in 2019, it has caused more than 664 million confirmed cases and 6.7 deaths globally by January 20, 2023. So far, novel undergone mutations from original, Alpha, Delta, Omicron strains. first detected South Africa November 2021 since spread rapidly to 100 countries around world, leading World Health Organization (WHO) classify as a “variant of concern” for COVID‐19. Due its stronger infectivity, faster transmission, higher...