A5100395301- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Ion Transport and Channel Regulation
- COVID-19 Clinical Research Studies
- Blood properties and coagulation
- Prion Diseases and Protein Misfolding
- Virus-based gene therapy research
- Neonatal Respiratory Health Research
- Viral gastroenteritis research and epidemiology
- Biosimilars and Bioanalytical Methods
- Cystic Fibrosis Research Advances
- Respiratory viral infections research
- Ion channel regulation and function
- Cancer Immunotherapy and Biomarkers
- Viral Infections and Immunology Research
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- PARP inhibition in cancer therapy
- RNA regulation and disease
- Orthopaedic implants and arthroplasty
- Cell death mechanisms and regulation
- Liver physiology and pathology
- Cervical Cancer and HPV Research
- Galectins and Cancer Biology
First Affiliated Hospital of Guangzhou Medical University
2023-2025
State Key Laboratory of Respiratory Disease
2023-2025
Guangzhou Medical University
2021-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2006-2024
University of Montana
2024
University of Colorado Anschutz Medical Campus
2021-2024
The Fourth People's Hospital of Ningxia Hui Autonomous Region
2022-2023
Zhejiang Chinese Medical University
2023
Soochow University
2022
Second People's Hospital of NanTong
2022
MurG is an essential glycosyltransferase that forms the glycosidic linkage between N- acetyl muramyl pentapeptide and glucosamine in biosynthesis of bacterial cell wall. This enzyme a member major superfamily NDP-glycosyltransferases for which no x-ray structures containing intact substrates have been reported. Here we present 2.5-Å crystal structure Escherichia coli complex with its donor substrate, UDP-GlcNAc. Combined genomic analysis other members site-specific mutagenesis E. MurG, this...
While the outcome of adoptive T cell therapy (ACT) is typically correlated with functionality inoculated cells, role endogenous cells unknown. The success checkpoint blockade has demonstrated potentially curative value preexisting tumor-primed in cancer treatment. Given results from therapy, we hypothesized that contribute to long-term survival following ACT. Here, describe a therapeutic approach combining ACT an oncolytic vaccine allows simultaneous analysis antitumor immunity mediated by...
Abstract The wide transmission and host adaptation of SARS-CoV-2 have led to the rapid accumulation mutations, posing significant challenges effectiveness vaccines therapeutic antibodies. Although several neutralizing antibodies were authorized for emergency clinical use, convalescent patients derived natural are vulnerable Spike mutation. Here, we describe screen a panel receptor-binding domain (RBD) targeted nanobodies (Nbs) from synthetic library design biparatopic Nb, named Nb1–Nb2, with...
Misfolded proteins destined for the cell surface are recognized and degraded by ERAD [ER (endoplasmic reticulum) associated degradation] pathway. TS (temperature-sensitive) mutants at permissive temperature escape reach surface. In this present paper, we examined a mutant of CFTR [CF (cystic fibrosis) transmembrane conductance regulator], ΔF508, analysed its cell-surface trafficking after rescue [rΔF508 (rescued ΔF508) CFTR]. We show that rΔF508 endocytosis is 6-fold more rapid (∼30% per 2.5...
Löfgren’s syndrome (LS) is an acute form of sarcoidosis characterized by a genetic association with HLA-DRB1*03 (HLA-DR3) and accumulation CD4+ T cells unknown specificity in the bronchoalveolar lavage (BAL). Here, we screened related LS-specific TCRs for antigen identified peptide derived from NAD-dependent histone deacetylase hst4 (NDPD) Aspergillus nidulans that stimulated these HLA-DR3–restricted manner. Using ELISPOT analysis, greater number IFN-γ– IL-2–secreting BAL DR3+ LS subjects...
Abstract Human coronaviruses (HCoVs) are a common etiological agent of acute respiratory tract infections. HCoV infections, especially those caused by the two HCoVs identified most recently, NL63 and HKU‐1, have not been characterized fully. To evaluate prevalence clinical presentations HKU1 in adults with an investigation infections Beijing, China from 2005 to 2009 was performed using reverse transcriptase PCR assays sequencing analysis. Among 8,396 specimens studied, 87 (1%) samples were...
A candidate vaccine (D1ME-VRP) expressing dengue virus type 1 premembrane and envelope proteins in a Venezuelan equine encephalitis (VEE) replicon particle (VRP) system was constructed tested conjunction with plasmid DNA (D1ME-DNA) identical sequences. Cynomolgus macaques were vaccinated three doses of (DDD), VRP (VVV group), or heterologous prime-VRP boost regimen (DDV) using two third dose vaccine. Four weeks after the final immunization, DDV group produced highest 1-specific...
We sought to establish whether the cystic fibrosis transmembrane conductance regulator (CFTR) regulates activity of amiloride-sensitive sodium channels (ENaC) in alveolar epithelial cells wild-type, heterozygous ( Cftr +/− ), knockout −/− and ΔF508-expressing mice situ. RT-PCR studies confirmed presence CFTR message freshly isolated type II (ATII) from wild-type mice. patched I (ATI) ATII prepared lung slices these demonstrated 4-pS ENaC with following basal open probabilities (P o ):...
Neutralizing antibody titers have been shown to correlate with immune protection against COVID-19 and can be used estimate vaccine effectiveness. Numerous studies explored the relationship between neutralizing antibodies protection. However, there remains a lack of quantitative data directly assessing minimum effective protective titer in vivo. In this study, we utilized eight cohorts participants diverse backgrounds for evaluation response. To precisely assess lower threshold required...
Inhaled formulations are the first choices for treating asthma and chronic obstructive pulmonary disease (COPD). Both local systemic exposures need to be considered when assessing efficacy safety of inhaled drugs. Physiologically-based pharmacokinetic (PBPK) models were constructed verified budesonide formoterol with different inhalation devices, CXG87 (test (T) formulation) Symbicort® Turbuhaler® (reference (R) formulation). The then used evaluate at peak inspiratory flow rates (PIFRs) dose...
The LIM-domain-binding protein Ldb1 is a key factor in the assembly of transcriptional complexes involving LIM-homeodomain proteins and other transcription factors that regulate animal development. We identified Ssdp (previously described as sequence-specific, single-stranded-DNA-binding proteins) components Ldb1-associated nuclear HeLa cells. are associated with variety additional mammalian cell types. This association specific, does not depend on presence nucleic acids, functionally...
The most common mutation in the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) gene, Delta F508, results production of a misfolded protein that is rapidly degraded. mutant temperature sensitive, and prior studies indicate low-temperature-rescued channel poorly responsive to physiological stimuli, degraded from cell surface at 37 degrees C. In present studies, we tested effect recently characterized pharmacological corrector,...
The mechanisms by which the exposure of mice to Cl(2) decreases vectorial Na(+) transport and fluid clearance across their distal lung spaces have not been elucidated. We examined biophysical, biochemical, physiological changes rodent epithelial channels (ENaCs) after Cl(2), identified involved. measured amiloride-sensitive short-circuit currents (I(amil)) isolated alveolar Type II (ATII) cell monolayers ENaC single-channel properties patching ATII ATI cells in situ. α-ENaC, γ-ENaC, total...
Tissue transglutaminase (TG2) mediates protein crosslinking through generation of ε−(γ-glutamyl) lysine isopeptide bonds and promotes cell adhesion interaction with fibronectin (FN) integrins. Cell to the peritoneal matrix regulated by TG2 facilitates ovarian cancer dissemination. Therefore, disruption TG2-FN complex small molecules may inhibit metastasis. A novel high throughput screening (HTS) assay based on AlphaLISA™ technology was developed measure formation a between His-TG2...
Despite its success in treating melanoma and hematological malignancies, adoptive cell therapy (ACT) has had only limited effects solid tumors. This is due part to a lack of specific antigen targets, poor trafficking infiltration, immunosuppression the tumor microenvironment. In this study, we combined ACT with oncolytic virus vaccines (OVVs) drive expansion infiltration transferred antigen-specific T cells demonstrated that combination highly potent for eradication established Consistent...
Immune recognition of tumor-expressed antigens by cytotoxic CD8+ T cells is the foundation adoptive cell therapy (ACT) and has been shown to elicit significant tumor regression. However, therapy-induced selective pressure can sculpt antigenicity tumors, resulting in outgrowth variants that lose target antigen. We demonstrate relapse from ACT subsequent oncolytic viral vaccination be prevented using class I HDACi, MS-275. Drug delivery subverted phenotype tumor-infiltrating CD11b+ Ly6Chi...
Benzo(a)pyrene (BaP) is a representative polycyclic aromatic hydrocarbon (PAH) compound, which has been implicated in cancer initiation and promotion. Although BaP one of the most extensively studied pollutants, underlying mechanisms through affects reactive oxygen species (ROS)/hypoxia-inducible factor 1α (HIF-1α)/heme oxygenase 1(HO-1) signaling during lung or breast carcinogenesis are not yet fully understood. In this study, we analyzed effects 0 (control), 1, 5, 25 µM exposure on A549...