A5055530532- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Advanced Glycation End Products research
- Alzheimer's disease research and treatments
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Diet, Metabolism, and Disease
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Bone health and treatments
- Immune cells in cancer
- Congenital heart defects research
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Developmental Biology and Gene Regulation
- Cancer Research and Treatments
- Muscle Physiology and Disorders
- Epigenetics and DNA Methylation
- Bone Metabolism and Diseases
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Amino Acid Enzymes and Metabolism
Wuhan University
2020-2025
China Medical University
2021-2025
Peking University First Hospital
2025
Peking University
2025
Center for Life Sciences
2025
Jiangxi Provincial Cancer Hospital
2025
University of California, San Diego
1995-2024
Lanzhou University
2023
Zhongnan Hospital of Wuhan University
2023
First People's Hospital of Yunnan Province
2023
Initiation, growth, recurrence, and metastasis of head neck squamous cell carcinomas (HNSCC) have been related to the behavior cancer stem cells (CSC) that can be identified by their aldehyde-dehydrogenase-isoform-1 (ALDH1) activity. We quantified enriched ALDH1+ within HNSCC lines subsequently characterized phenotypical functional properties like invasion capacity epithelial-mesenchymal transition (EMT). Spheroid culture CSC from five up 5-fold. In spheroid-derived (SDC) parental...
Abstract Cancer-secreted, extracellular vesicle (EV)–encapsulated miRNAs enable cancer cells to communicate with each other and noncancerous in tumor pathogenesis response therapies. Here, we show that treatment a sublethal dose of chemotherapeutic agents induces breast secrete EV the capacity stimulate stem-like cell (CSC) phenotype, rendering resistance therapy. Chemotherapy induced multiple miRNAs, including miR-9-5p, miR-195-5p, miR-203a-3p, which simultaneously targeted transcription...
Abstract Background D-ribose in cells and human serum participates glycation of proteins resulting advanced end products (AGEs) that affect cell metabolism induce death. However, the mechanism by which D-ribose-glycated death is still unclear. Results Here, we incubated with bovine albumin (BSA) observed changes intensity fluorescence at 410 nm 425 to monitor formation BSA. Comparing BSA xylose (a control for furanose), glucose fructose (controls pyranose), rate was most rapid. Protein...
Abstract Extracellular vesicles (EVs) actively participate in intercellular communication and pathological processes. Studying the molecular signatures of EVs is key to reveal their biological functions clinical values, which, however, greatly hindered by sub‐100 nm dimensions, low quantities biomolecules each EV carries, large population heterogeneity. Now, single‐EV flow cytometry analysis introduced realize single counting phenotyping a conventional cytometer for first time, enabled...
Hyperphosphorylated tau has a critical role in tauopathies such as Alzheimer's disease and frontotemporal dementia, impairing neuronal function eventually leading to neurodegeneration. A for is supported by studies transgenic mouse models that express the P301L mutation found cases of familial with accumulation hyperphosphorylated hippocampus causing reductions hippocampal long-term potentiation impairments spatial learning memory. However, what remained unexplored reducing excitability....
Mitochondria are the powerhouse of eukaryotic cells, which regulate cell metabolism and differentiation. Recently, mitochondrial transfer between cells has been shown to direct recipient fate. However, it is unclear whether mitochondria can translocate stem this alters Here, mesenchymal (MSC) regulation examined by macrophages in bone marrow environment. It found that promote osteogenic differentiation MSCs delivering MSCs. under osteoporotic conditions, with altered phenotypes, metabolic...
Abstract Cancer-associated cachexia is a multi-organ weight loss syndrome, especially with wasting disorder of adipose tissue and skeletal muscle. Small extracellular vesicles (sEVs) serve as emerging messengers to connect primary tumour metabolic organs exert systemic regulation. However, whether how tumour-derived sEVs regulate white (WAT) browning fat poorly defined. Here, we report breast cancer cell-secreted exosomal miR-204-5p induces hypoxia-inducible factor 1A (HIF1A) in WAT by...
Abstract Breast cancer metastasis to the brain is a clinical challenge rising in prevalence. However, underlying mechanisms, especially how cells adapt distant niche facilitate colonization, remain poorly understood. A unique metabolic feature of coupling between neurons and astrocytes through glutamate, glutamine, lactate. Here we show that extracellular vesicles from breast with high potential develop metastases carry levels miR-199b-5p, which shows higher blood patients comparing those...
A regular heart beat is dependent on a specialized network of pacemaking and conductive cells. There has been longstanding controversy regarding the developmental origin these cardiac tissues which also manifest neural-like properties. Recently, we have shown conclusively that during chicken embryogenesis, impulse-conducting Purkinje cells are recruited from myocytes in spatial association with developing coronary arteries. Here, report cultured embryonic convert to cell phenotype after...
Tinman is a Drosophila homeodomain protein that required for formation of both visceral and cardiac mesoderm, including the dorsal vessel, heart-like organ. Although several vertebrate tinman homologues have been characterized, their requirement in earliest stages heart has an open question, perhaps complicated by potential functional redundancy homologues. We utilized novel approach to investigate within gene family, coinjecting DNA encoding dominantly acting repressor derivatives specific...
tinman is a Drosophila Nk-homeobox gene required for heart and visceral mesoderm specification. Mutations in result lack of formation the heart, dorsal vessel. We have isolated an from Xenopus laevis, XNkx-2.3, which appears by sequence homology expression pattern to be homologue tinman. The XNKx-2.3 both during development adult tissues partially overlaps with that another homologue, Csx/NKx-2.5/XNkx-2.5. found embryonic XNkx-2.3 XNkx-2.5 induced at time when cardiac specification...
Tau, an important microtubule associated protein, has been found to bind DNA, and be localized in the nuclei of both neurons some non-neuronal cells. Here, using electrophoretic mobility shifting assay (EMSA) presence DNA with different chain-lengths, we observed that tau protein favored binding a 13 bp or longer polynucleotide. The results from atomic force microscopy also showed preferred polynucleotide 12 shorter In competitive assay, minor groove binder distamycin A was able replace...
Alpha synuclein (alpha-Syn) is the main component of Lewy bodies which are associated with several neurodegenerative diseases such as Parkinson's disease. While glycation D-glucose that results in alpha-Syn misfold and aggregation has been studied, effects D-ribose on have not investigated.Here, we show ribosylation induces misfolding generates advanced end products (AGEs) form protein molten globules high cytotoxcity. Results from native- SDS-PAGE showed reacted rapidly alpha-Syn, leading...
Bone is one of the most frequent metastatic sites advanced breast cancer. Current therapeutic agents aim to inhibit osteoclast-mediated bone resorption but only have palliative effects. During normal remodeling, balance between and osteoblast-mediated formation essential for homeostasis. One major function osteoblast during secrete type I procollagen, which will then be processed before being crosslinked deposited into matrix. Small RNA sequencing quantitative real-time PCR were used detect...