A5060003523- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Neonatal and Maternal Infections
- COVID-19 Clinical Research Studies
- Blood disorders and treatments
- Platelet Disorders and Treatments
- Inflammation biomarkers and pathways
- Congenital Diaphragmatic Hernia Studies
- Respiratory Support and Mechanisms
- Cell Adhesion Molecules Research
- Inflammasome and immune disorders
- Nitric Oxide and Endothelin Effects
- Blood Coagulation and Thrombosis Mechanisms
- Protease and Inhibitor Mechanisms
- S100 Proteins and Annexins
- Sepsis Diagnosis and Treatment
- Innovations in Medical Education
- Neuroinflammation and Neurodegeneration Mechanisms
- Vasculitis and related conditions
- Congenital Heart Disease Studies
- Infant Nutrition and Health
- Neuroscience of respiration and sleep
- Child Nutrition and Water Access
- Cardiac Arrest and Resuscitation
University of Utah
2015-2025
Primary Children's Hospital
2005-2025
Intermountain Healthcare
2022-2025
Pediatrics and Genetics
2011-2021
Geriatric Research Education and Clinical Center
2020
Institute of Molecular Medicine
2011-2016
Williams (United States)
2005
Fitzsimons Army Medical Center
1980
Coronavirus disease 2019 (COVID-19) is a novel, viral-induced respiratory that in ∼10-15% of patients progresses to acute distress syndrome (ARDS) triggered by cytokine storm. In this Perspective, autopsy results and literature are presented supporting the hypothesis little known yet powerful function neutrophils-the ability form neutrophil extracellular traps (NETs)-may contribute organ damage mortality COVID-19. We show lung infiltration neutrophils an specimen from patient who succumbed...
Human β-defensins (hBD) are antimicrobial peptides that curb microbial activity. Although hBD's primarily expressed by epithelial cells, we show human platelets express hBD-1 has both predicted and novel antibacterial activities. We observed activated surround Staphylococcus aureus (S. aureus), forcing the pathogens into clusters have a reduced growth rate compared to S. alone. Given microbicidal activity of β-defensins, determined whether hBD family members were present in found mRNA...
Ischemic stroke prompts a strong inflammatory response, which is associated with exacerbated outcomes. In this study, we investigated mechanistic regulators of neutrophil extracellular trap (NET) formation in and whether they contribute to NET-forming neutrophils were found throughout brain tissue ischemic patients, elevated plasma NET biomarkers correlated worse Additionally, observed increased platelet surface-expressed high-mobility group box 1 (HMGB1) patients. Mechanistically, platelets...
Neutrophil granulocytes, also called polymorphonuclear leukocytes (PMNs), extrude molecular lattices of decondensed chromatin studded with histones, granule enzymes, and antimicrobial peptides that are referred to as neutrophil extracellular traps (NETs). NETs capture contain bacteria, viruses, other pathogens. Nevertheless, experimental evidence indicates cause inflammatory vascular tissue damage, suggesting identifying pathways inhibit NET formation may have therapeutic implications. Here,...
Coronavirus disease 2019 (COVID-19) is associated with derangement in biomarkers of coagulation and endothelial function has been likened to the coagulopathy sepsis. However, clinical laboratory metrics suggest key differences these pathologies. We sought determine whether plasma fibrinolytic potential patients COVID-19 differ compared healthy donors critically ill Approach Results: performed comparative studies on plasmas from a single-center, cross-sectional observational study 99...
COVID-19 severity and its late complications continue to be poorly understood. Neutrophil extracellular traps (NETs) form in acute COVID-19, likely contributing morbidity mortality.This study evaluated immunothrombosis markers a comprehensive cohort of recovered patients, including the association NETs with long COVID.One-hundred-seventy-seven patients were recruited from clinical cohorts at 2 Israeli centers: (mild/moderate, severe/critical), convalescent (recovered COVID), along 54...
A randomized controlled investigation was undertaken to evaluate the role of maternal oral antibiotic therapy in decreasing incidence and neonatal colonization at term with group B β-hemolytic streptococcus (GB-BHS). Data were collected determine optimum transfer media rate study population. At delivery 1441 maternal-infant pairs evaluated. One hundred sixty-eight women (11.6%) 55 infants (3.8%) colonized. Forty-four colonized GB-BHS 38 weeks' gestation randomly assigned a treatment (500 mg...
To assess the incidence and clinical outcomes of neonates with congenital diaphragmatic hernia patients concurrent bronchopulmonary sequestration (CDH + BPS). In this retrospective cohort study, we compared BPS diagnoses in CDH across 4 epochs (2002-2008, 2009-2015, 2016-2019, 2020-2023) to BPS. Because marked epoch differences key outcomes, only extracorporeal membrane oxygenation use survival for alone final 2 using multinominal regression analysis. Among 383 CDH, concurrently diagnosed 15...
Abstract Background Exome/genome sequencing (ES/GS) have been recently used in neonatal and pediatric/cardiac intensive care units (NICU PICU/CICU) to diagnose for acutely ill infants, but the effectiveness of targeted gene panels these purposes remains unknown. Methods RapSeq, a newly developed panel targeting 4,503 disease‐causing genes, was employed on selected patients our NICU/PICU/CICU. Twenty trios were sequenced from October 2015 March 2017. We assessed diagnostic yield, turnaround...
Protease activated receptor (PAR) 4 (gene: F2RL3) harbors a functional dimorphism, rs773902 A/G (encoding Thr120/Ala120, respectively) and is associated with greater platelet aggregation. The A allele frequency more common in Black individuals, individuals have higher incidence of ischemic stroke than White individuals. However, it not recognized whether the responsible for worse outcomes. To directly test vivo effect this variant on stroke, we generated mice where F2rl3 was replaced by...
The mechanisms by which neutrophils, key effector cells of the innate immune system, express new gene products in inflammation are largely uncharacterized. We found that they rapidly translate constitutive mRNAs when activated, a previously unrecognized response. One proteins synthesized without requirement for transcription is soluble IL-6 receptor α, translocates to endothelial and induces temporal switch mononuclear leukocyte recruitment. Its synthesis regulated specialized translational...
In addition to releasing preformed granular proteins, polymorphonuclear leukocytes (PMNs) synthesize chemokines and other factors under transcriptional control. Here we demonstrate that PMNs express an inducible modulator by signal-dependent activation of specialized mechanisms regulate messenger RNA (mRNA) translation. HL-60 myelocytic cells differentiated surrogate respond platelet activating factor initiating translation with appearance specific mRNA transcripts in polyribosomes. cDNA...
Dysregulation of the inflammatory response against infection contributes to mortality in sepsis. Inflammation provides critical host defense, but it can cause tissue damage, multiple organ failure, and death. Because nuclear transcription factor peroxisome proliferator-activated receptor γ (PPARγ) exhibits therapeutic potential, we characterized role PPARγ We analyzed severity clinical signs, survival rates, cytokine production, leukocyte influx, bacterial clearance a cecal ligation puncture...