A5011411239- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- PI3K/AKT/mTOR signaling in cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood properties and coagulation
- Cancer-related molecular mechanisms research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Complement system in diseases
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- MicroRNA in disease regulation
- Mast cells and histamine
- Protein Kinase Regulation and GTPase Signaling
- Extracellular vesicles in disease
- Blood Coagulation and Thrombosis Mechanisms
- Immune Response and Inflammation
- Acute Ischemic Stroke Management
- Protease and Inhibitor Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Atherosclerosis and Cardiovascular Diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Fungal Plant Pathogen Control
- Monoclonal and Polyclonal Antibodies Research
- Inflammasome and immune disorders
University of Utah
2015-2024
Centro de Investigaciones Biológicas Margarita Salas
2023
Consejo Superior de Investigaciones Científicas
2023
Geriatric Research Education and Clinical Center
2020
Temple University
2013-2019
Universität Greifswald
2018
Paul Ehrlich Institut
2018
George E. Wahlen Department of VA Medical Center
2018
Intermountain Medical Center
2017
Czech Academy of Sciences, Institute of Physiology
2014
Vascular injury has emerged as a complication contributing to morbidity in coronavirus disease 2019 (COVID-19). The glycosaminoglycan hyaluronan (HA) is major component of the glycocalyx, protective layer glycoconjugates that lines vascular lumen and regulates key endothelial cell functions. During critical illness, case sepsis, enzymes degrade releasing fragments with pathologic activities into circulation thereby exacerbating disease. Here, we analyzed levels circulating glycosaminoglycans...
Abstract Dysregulated inflammation is implicated in the pathobiology of aging, yet platelet–leukocyte interactions and downstream cytokine synthesis aging remains poorly understood. Platelets monocytes were isolated from healthy younger (age <45, n = 37) older ≥65, 30) adults incubated together under autologous nonautologous conditions. Synthesis inflammatory cytokines by monocytes, alone or presence platelets, was examined. Next-generation RNA-sequencing allowed for unbiased...
3-phosphoinositide-dependent protein kinase 1 (PDK1), a member of the A,G and C (AGC) family proteins, is Ser/Thr that can phosphorylate activate other kinases from AGC family, including Akt at Thr308, all which play important roles in mediating cellular responses. The functional role PDK1 or importance phosphorylation on Thr308 for its activity has not been investigated human platelets. In this study, we tested two pharmacological inhibitors PDK1, BX795 BX912, to assess Akt. PAR4-induced...
Platelets and megakaryocytes are critical players in immune responses. Recent reports suggest infection inflammation alter the megakaryocyte platelet transcriptome to induce altered reactivity. We determined whether nonviral sepsis induces differential gene expression Nonviral upregulated IFN-induced transmembrane protein 3 (IFITM3), an IFN-responsive that restricts viral replication. As IFITM3 has been linked clathrin-mediated endocytosis, we promoted endocytosis of α-granule proteins. IFN...
BACKGROUND: Single-nucleotide polymorphisms linked with the rs1474868 T allele ( MFN2 [mitofusin-2] T/T) in human mitochondrial fusion protein gene are associated reduced platelet RNA expression and counts. This study investigates impact of on megakaryocyte biology. METHODS: Mice megakaryocyte/platelet deletion Mfn2 −/− [ conditional knockout]) were generated using Pf4-Cre crossed floxed mice. Human megakaryocytes from cord blood platelets isolated healthy subjects genotyped for rs1474868....
Platelets are activated by a range of stimuli that share little or no resemblance in structure to each other recognized ligands, including diesel exhaust particles (DEP), small peptides [4N1-1, Champs (computed helical anti-membrane proteins), LSARLAF (Leu-Ser-Ala-Arg-Leu-Ala-Phe)], proteins (histones) and large polysaccharides (fucoidan, dextran sulfate). This miscellaneous group stimulate aggregation human mouse platelets through the glycoprotein VI (GPVI)-FcR γ-chain complex and/or C-type...
Gαq plays an important role in platelet activation by agonists such as thrombin, adenosine diphosphate (ADP) and thromboxane. The significance of signaling platelets was established using YM254890, a Gαq/11-specific inhibitor knockout murine platelets. However, YM-254890 is no longer available for investigators there need to characterize other inhibitors. aim this study the specificity compound,...
One source of endogenous reverse transcriptase (eRT) activity in nucleated cells is the LINE-1/L1 (long interspersed nuclear element-1), a non-LTR retrotransposon that implicated regulation gene expression. Nevertheless, presence and function eRT LINE-1 human platelets, an anucleate cell, has not previously been determined.