A5071515803- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Phagocytosis and Immune Regulation
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Probiotics and Fermented Foods
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Meat and Animal Product Quality
- Fatty Acid Research and Health
- Neonatal Respiratory Health Research
- interferon and immune responses
- Lymphatic System and Diseases
- Urticaria and Related Conditions
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Viral Infections and Outbreaks Research
- RNA regulation and disease
- Multiple Myeloma Research and Treatments
Monash University
2017-2024
Australian Regenerative Medicine Institute
2024
Centre National de la Recherche Scientifique
2021
Université Paris-Saclay
2021
Université Paris-Sud
2021
Walter and Eliza Hall Institute of Medical Research
2014-2021
The University of Melbourne
2014-2021
Inserm
2021
Université de Strasbourg
2021
Institut Curie
2021
Mitochondrial apoptosis is mediated by BAK and BAX, two proteins that induce mitochondrial outer membrane permeabilization, leading to cytochrome c release activation of apoptotic caspases. In the absence active caspases, DNA (mtDNA) triggers innate immune cGAS/STING pathway, causing dying cells secrete type I interferon. How cGAS gains access mtDNA remains unclear. We used live-cell lattice light-sheet microscopy examine network in mouse embryonic fibroblasts. found after BAK/BAX loss,...
Significance Group 3 innate lymphoid cells (ILC3s) play decisive roles in mammalian physiology including tissue repair, development, and immune regulation. So far, the functions of ILC3s adult system have been mainly linked to their capacity release cytokines response microbial or inflammatory signals. The results presented here show that activated can alter outcome adaptive responses by directly stimulating CD4 + T cells. Indeed, IL-1β–activated express costimulatory molecules induce...
Intrinsic apoptosis is critical to prevent tumor formation and engaged by many anti-cancer agents eliminate cells. BAX BAK, the two essential mediators of apoptosis, are thought be regulated through similar mechanisms act redundantly drive apoptotic cell death. From an unbiased genome-wide CRISPR/Cas9 screen, we identified VDAC2 (voltage-dependent anion channel 2) as important for BAX, but not function. Genetic deletion abrogated association BAK with mitochondrial complexes containing VDAC1,...
Abstract We used a comprehensive collection of Affymetrix microarray datasets to ascertain which genes or molecules distinguish the known major subsets human T cells. Our strategy allowed us identify expressed in most cell subsets: TCR αβ+ and γδ+, three effector (Th1, Th2, follicular helper cells), central memory, activated cells, others. genechip dataset also for identification preferentially exclusively by compared with numerous non-T leukocyte profiled. Cross-comparisons between revealed...
Abstract During fetal life, CD4+CD3− lymphoid tissue inducer (LTi) cells are required for lymph node and Peyer’s patch development in mice. In adult animals, found low numbers organs. Whether LTi generated maintained through cytokine signals has not been directly addressed. this study we show that adoptively transferred into neonatal CXCR5−/− mice induced the formation of intestinal tissues, demonstrating first time their bona fide function. Increasing IL-7 availability wild-type either by...
Platelets are anuclear cells that essential for blood clotting. They produced by large polyploid precursor called megakaryocytes. Previous genome-wide association studies in nearly 70,000 individuals indicated single nucleotide variants (SNVs) the gene encoding actin cytoskeletal regulator tropomyosin 4 (TPM4) exert an effect on count and volume of platelets. Platelet number independent risk factors heart attack stroke. Here, we have identified 2 unrelated families BRIDGE Bleeding Disorders...
Abstract Genetic or vitamin D3‐induced overexpression of thymic stromal lymphopoietin (TSLP) by keratinocytes results in an atopic dermatitis (AD)‐like inflammatory phenotype mice echoing the discovery high TSLP expression epidermis from AD patients. Although skin dendritic cells (DC) are suspected to be involved AD, direct evidence a pathogenetic role for DC TSLP‐induced inflammation has not yet been demonstrated. In mouse model i.e. treated with low‐calcemic D3 analogue, MC903, we show...
Lymph node (LN) organogenesis is initiated by the interaction between hematopoietic lymphoid tissue inducer (LTi) cells and mesenchymal organizer cells. Mice in which IL-7 signaling pathway has been disrupted have a severe defect LN development; however, reasons underlying this are as yet unknown. In study, we show that overexpression of thymic stromal lymphopoietin (TSLP) increased LTi cell numbers restored development IL-7(-/-) RAG2(-/-) gamma(c)(-/-) mice. The TSLP-mediated restoration...
<h3>Abstract</h3> Type-1 interferons (IFNs) are pleiotropic cytokines that signal through the type-1 IFN receptor (IFNAR1). Recent literature has implicated IFNs in disorders of CNS. In this study, we have investigated role neuroinflammation following traumatic brain injury (TBI). Using a controlled cortical impact model, TBI was induced 8- to 10-week-old male C57BL/6J WT and IFNAR1<sup>−/−</sup> mice brains were excised study infarct volume, inflammatory mediator release via quantitative...
Thymic epithelial cells provide unique cues for the lifelong selection and differentiation of a repertoire functionally diverse T cells. Rendered microRNA (miRNA) deficient, these stromal in mouse lose their capacity to instruct commitment hematopoietic precursors cell fate, effect thymocyte positive selection, achieve promiscuous gene expression required central tolerance induction. Over time, microenvironment created by miRNA-deficient thymic epithelia assumes cellular composition...
Due to the myriad interactions between prosurvival and proapoptotic members of Bcl-2 family proteins, establishing mechanisms that regulate intrinsic apoptotic pathway has proven challenging. Mechanistic insights have primarily been gleaned from in vitro studies because genetic approaches mammals produce unambiguous data are difficult design. Here we describe a mutation mouse human Bak specifically disrupts its interaction with protein Bcl-xL Substitution Glu75 mBak (hBAK Q77) for leucine...
During platelet biogenesis, microtubules (MTs) are arranged into submembranous structures (the marginal band) that encircle the cell in a single plane. This unique MT array has no equivalent any other mammalian cell, and mechanisms responsible for this particular mode of assembly not fully understood. One possibility is MTs composed set tubulin isotypes carry specific posttranslational modifications. Although β1-tubulin known to be essential, roles α-tubulin formation or function have so far...
Hematopoietic lymphoid tissue inducer (LTi) cells initiate lymph node (LN) and Peyer's patch (PP) development during fetal life by inducing the differentiation of mesenchymal organizer cells. The growth factor signals underlying LTi cell LN PP organogenesis remain poorly understood. express Il7r receptor tyrosine kinase Kit, whereas their cognate ligands. To determine relative significance Il7 Kit signaling in homeostasis development, we have analyzed mice deficient for (Kit(W/Wv)),...
Interleukin-7 (IL-7) is a major survival factor for mature T cells. Therefore, the degree of IL-7 availability determines size peripheral cell pool and regulates homeostasis. Here we provide evidence that also homeostasis intestinal epithelial cells (IEC), colon function composition commensal microflora. In cell-deficient, lymphopenic mice, IL-7-producing IEC accumulate. hyperplasia can be blocked by IL-7-consuming or inactivation IL-7/IL-7R signaling pathway. However, blockade pathway...
In a study of the evolution conjugated linoleic acid (CLA) during cheese production, influence Emmental processing on CLA content and isomer composition was evaluated. The use raw thermised milk, changes temperature effect propionic bacteria (PAB) were investigated. in milk 8·6±1·9 mg/g fat ripened at 70 d 8·6±1·6 fat, under normal conditions. No observed manufacturing process. Changes cooking moulding temperatures did not content. made from microfiltered with two different Propionibacterium...
Abstract IgH genes are assembled during early B cell development by a series of regulated DNA recombination reactions in which DH and JH segments first joined followed VH to DJH rearrangement. Recent studies have highlighted the role chromatin structure control V(D)J recombination. In this study, we show that, murine pro-B precursors, located within 6-kb DNase I-sensitive domain containing acetylated histones H3 H4, is delimited 5′ DQ52 promoter element 3′ intronic enhancer. Within domain,...