A5039912831- Neuroinflammation and Neurodegeneration Mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
- Parkinson's Disease Mechanisms and Treatments
- Hearing Loss and Rehabilitation
- Immune Response and Inflammation
- Ion Channels and Receptors
- Neurological diseases and metabolism
- Receptor Mechanisms and Signaling
- interferon and immune responses
- Virus-based gene therapy research
- Nuclear Receptors and Signaling
- Herbal Medicine Research Studies
- RNA Interference and Gene Delivery
- Atherosclerosis and Cardiovascular Diseases
- Sexual Differentiation and Disorders
- Renin-Angiotensin System Studies
- Advanced Fluorescence Microscopy Techniques
- Hormonal Regulation and Hypertension
- Steroid Chemistry and Biochemistry
- Nerve injury and regeneration
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Planarian Biology and Electrostimulation
- Alzheimer's disease research and treatments
- Immune Cell Function and Interaction
- Advanced Adaptive Filtering Techniques
The University of Melbourne
2012-2023
The Royal Victorian Eye & Ear Hospital
2019-2023
Australian Research Council
2012
Murdoch Children's Research Institute
2008-2012
Royal Children's Hospital
2009
Children's Cancer Center
1999
Baylor College of Medicine
1999
University of Southern California
1999
A number of transient receptor potential (TRP) channels has recently been shown to mediate cutaneous thermosensitivity. Sensitivity warm and cool stimuli demonstrated in both human animal gastrointestinal tract; however, the molecular mechanisms that underlie this have not determined. Vagal afferent neurons with cell bodies nodose ganglion are known nonnociceptive sensation from upper gut. In study, isolated cultured mouse showed changes cytoplasmic-free Ca(2+) concentrations over a range...
Type‐1 interferons (IFNs) are pleiotropic cytokines with a critical role in the initiation and regulation of pro‐inflammatory response. However, contribution type‐1 IFNs to CNS disorders, specifically chronic neuropathologies such as Parkinson's disease is still unknown. Here, we report increased IFN signaling both post mortem human samples 1‐methyl‐4‐phenyl‐1, 2, 3, 6‐tetrahydropyridine (MPTP) mouse model. In response MPTP, mice lacking receptor (IFNAR1 −/− ) displayed decreased signaling,...
A neuro-inflammatory response is evident in Alzheimer's disease (AD), yet the precise mechanisms by which neuro-inflammation influences progression of (AD) remain poorly understood. Type-1 interferons (IFNs) are master regulators innate immunity and have been implicated multiple CNS disorders, however their role AD has not fully investigated. Hence, we generated APPSWE/PS1ΔE9 mice lacking type-1 IFN alpha receptor-1 (IFNAR1, x IFNAR1−/−) aged to 9 months investigate signaling a...
<h3>Abstract</h3> Type-1 interferons (IFNs) are pleiotropic cytokines that signal through the type-1 IFN receptor (IFNAR1). Recent literature has implicated IFNs in disorders of CNS. In this study, we have investigated role neuroinflammation following traumatic brain injury (TBI). Using a controlled cortical impact model, TBI was induced 8- to 10-week-old male C57BL/6J WT and IFNAR1<sup>−/−</sup> mice brains were excised study infarct volume, inflammatory mediator release via quantitative...
Neuro-inflammation has long been implicated as a contributor to the progression of Alzheimer's disease in both humans and animal models. Type-1 interferons (IFNs) are pleiotropic cytokines critical mediating innate immune pro-inflammatory response. The production type-1 IFNs following pathogen detection is, part, through activation toll-like receptors (TLRs) subsequent signalling myeloid differentiation factor-88 (Myd88) interferon regulatory factors (IRFs). We have previously identified...
Evidence from post-mortem human brains, animal studies and cell culture models has implicated neuroinflammation in the aetiology of chronic neuropathologies including Alzheimer's Parkinson's diseases. Although neuroinflammatory response is considered detrimental contributing to these pathologies, underlying mechanisms are still not well understood. The type-I interferons (IFNs) have been characterised periphery known initiate/modulate immune response. Recently, they ageing we also...
Visualisation of cochlear histopathology in three-dimensions has been long desired the field hearing research. This paper outlines a technique that made this possible and shows research application protection after implantation. The utilises robust immunofluorescent labelling followed by effective tissue clearing fast image acquisition using Light Sheet Microscopy. We can access health individual components detection proteins such as myosin VIIa to look at hair cells, NaKATPase alpha 3...
Cochlear implantation leads to many structural changes within the cochlea which can impair residual hearing. In patients with preserved low-frequency hearing, a delayed hearing loss occur weeks-to-years post-implantation. We explore whether stiffening of basilar membrane (BM) may be contributory factor in an animal model. Our objective is map morphology and Young’s modulus basal apical areas BM after cochlear implantation, using quantitative nanomechanical atomic force microscopy (QNM-AFM)...
Current clinical gene therapy protocols for the treatment of human immunodeficiency virus type 1 (HIV-1) infection involve ex vivo transduction and expansion CD4+ T cells derived from HIV-positive patients at a late stage in their disease (CD4+ cell count <400 cells/mm3). We examined efficiency transgene expression adult bone marrow (BM)- umbilical cord blood (UCB)-derived CD34+ induced to differentiate into monocytes vitro with an MuLV-based vector encoding neomycin resistance intracellular...
Abstract Fluorescent selective angiotensin AT 1 receptor antagonists (sartans) that contain naphthalene ( 6 , 7 10 and 11 ), anthracene 12 14 acenaphthene 13 15 coumarin 8 9 ) substituents have been prepared their preliminary pharmacology evaluated in Chinese hamster ovary (CHO) cell based assays. Compounds – proved to be effective sartans with p K B estimates the range of 6.7–10.5; potential as cellular imaging agents is also discussed.
Introduction Intraoperative trauma leading to bleeding during cochlear implantation negatively impacts residual hearing of implant recipients. There are no clinical protocols for the removal blood implantation, reduce consequential effects such as inflammation and fibrosis which adversely affect health hearing. This preclinical study investigated implementation an intra‐cochlear flushing protocol blood. Methods Three groups guinea pigs were studied 28 days after implantation; implant‐only...
IFNβ mRNA transcript levels are unaltered in cortical tissue 9 month old APPSWE/PS1ΔE9 mice. Q-PCR of isolated from wildtype and mice analyzing levels. For Q-PCR, all samples were normalized back to the Ct value housekeeping gene GAPDH (ΔCt). The variant genotype groups then expressed relative their gene-specific littermate controls (fold change, ΔΔCt). Data displayed as box plots described statistical analysis section Materials Methods (n = 7 per genotype) See Additional file 2: Table S1...