A5036734580- Hypothalamic control of reproductive hormones
- Hormonal Regulation and Hypertension
- Neuropeptides and Animal Physiology
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Testicular diseases and treatments
- Neuroblastoma Research and Treatments
- Birth, Development, and Health
- Adenosine and Purinergic Signaling
- Receptor Mechanisms and Signaling
- Growth Hormone and Insulin-like Growth Factors
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Vitamin K Research Studies
- Ovarian function and disorders
- Venous Thromboembolism Diagnosis and Management
- Cardiac tumors and thrombi
- Sexual Differentiation and Disorders
- Neuroendocrine regulation and behavior
- Body Contouring and Surgery
- Blood Coagulation and Thrombosis Mechanisms
- Lymphoma Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Angiogenesis and VEGF in Cancer
- Effects and risks of endocrine disrupting chemicals
Peter Doherty Institute
2024-2025
The University of Melbourne
2024-2025
Universität Hamburg
1992-2023
University Medical Center Hamburg-Eppendorf
2022-2023
University Cancer Center Hamburg
2023
Bristol Royal Infirmary
1995-1996
University of Bristol
1994-1996
Pituitary adenylate cyclase-activating polypeptide (PACAP) is thought to play a hypophysiotropic role, but little known of the identity PACAP-stimulated cells in pituitary, nature PACAP receptors on specific cell types, and effector systems for these receptors. Here we describe effects alpha T3-1 cells, gonadotrope-derived line. In PACAP38 causes concentration-dependent increases cAMP accumulation (EC50, 3 nM), [3H]inositol phosphate ([3H]IP) production 20 cytosolic Ca2+ concentration. The...
Stem-like T cells are attractive immunotherapeutic targets in patients with cancer given their ability to proliferate and differentiate into effector progeny. Thus, identifying enhanced stemness understanding developmental requirements of broad clinical therapeutic interest. Here, we demonstrate that during acute infection, the transcriptional regulator inhibitor DNA binding 3 (ID3) identifies stem-like uniquely adapted generate precursors exhausted (Tpex) response chronic infection or...
Despite a rapidly increasing acceptance for role of ATP as an extracellular mediator in several biological systems, the present report shows that may mediate physiological responses pituitary cells. We have now been able to demonstrate specific action receptors release luteinizing hormone from gonadotropes and coupled them with further studies clearly show can be exocytotically released cultured rat Both UTP (100 microM) caused > 14-fold increase rate superfused Adenosine 5'-[alpha,...
Sustained exposure of gonadotropes to GnRH causes a pronounced desensitization GnRH-stimulated gonadotropin release, but the mechanisms involved are poorly understood. Recent studies have suggested, however, that phosphoinositidase C (PIC) activity does not undergo rapid ( < 5 min) homologous in alpha T3-1 cells, and we have, therefore, used this cell line address question whether occurs distal PIC and/or an intermediate time frame. We show stimulates increase inositol 1,4,5-trisphosphate...
Natriuretic peptides act via receptors with intrinsic guanylate cyclase activity to stimulate cGMP production and are thought be important regulators of neuroendocrine systems. C-Type natriuretic peptide (CNP) is particular interest in this regard because the highest tissue concentrations CNP occur anterior pituitary, where it a highly potent stimulator production. Here we show that pituitaries rats mice contain abundant prohormone messenger RNA (mRNA), but no atrial or B-type mRNAs. Using...
Atrial, brain-type, and C-type natriuretic peptides (ANP, BNP, CNP) act via receptors with intrinsic guanylate cyclase activity. The A-type B-type ANP are selectively activated by CNP, respectively. anterior pituitary is a site of production action, suggesting local regulatory function, this may also hold true for CNP which found at its highest tissue concentrations in the pituitary. Here we show that these all cause dose-dependent increases cGMP accumulation alpha T3-1 cells (a...
Pituitary adenylate cyclase-activating polypeptide (PACAP) acts via type I receptors in the pituitary to stimulate cAMP production. Gonadotropes are likely target cells for PACAP action, and we have recently shown alpha T3-1 cells, a clonal gonadotrope-derived cell line, be responsive. Here explored influence of GnRH on action show that PACAP38-stimulated production is inhibited by both presence absence phosphodiesterase inhibitor. This effect appears not Ca++ mediated but mimicked protein...
We have recently identified gonadotropes as target cells for ATP action via receptors of the P2U subtype. The present studies used gonadotrope-derived alpha T3-1 to examine possible signaling mechanisms subserving in gonadotropes. Addition produced a biphasic intracellular Ca2+ (Ca2+i) response: transient spike followed by small plateau. Removal extracellular or depolarization with KCl abolished plateau but had no effect on spike. was also blocked cadmium nifedipine not nickel. Pretreatment...
Journal Article Dependence of gonadotropin-releasing hormone-stimulated luteinizing hormone release upon intracellular Ca2+ pools is revealed by desensitization and thapsigargin blockade. Get access C A McArdle, McArdle 1Institute for Hormone Fertility Research, Hamburg, Germany. Search other works this author on: Oxford Academic Google Scholar Poch Endocrinology, Volume 130, Issue 6, 1 June 1992, Pages 3567–3574, https://doi.org/10.1210/endo.130.6.1534542 Published: 01 1992
Abstract In pituitary gonadotrophs GnRH causes biphasic (spike and plateau) increases in cytosolic Ca 2+ ([Ca ] i ) gonadotrophin release. The spike phases reflect mobilization of stored the plateau responses are attributed, part, to influx via voltage-sensitive channels. recent years, store-dependent (SDCI), which depletion intracellular inositol 1,4,5-trisphosphate-mobilizable pool stimulates influx, has emerged as a major form entry activated by phosphoinositidase C-coupled receptors...
Abstract Venetoclax, a first-in-class BH3 mimetic drug targeting BCL-2, has improved outcomes for patients with chronic lymphocytic leukemia (CLL). Early measurements of the depth venetoclax treatment response, assessed by minimal residual disease, are strong predictors long-term clinical outcomes. Yet, there limited data concerning early changes induced that might inform strategies to improve responses. To address this gap, we conducted longitudinal mass cytometric profiling blood cells...
Although cancer-associated thrombosis (CAT) is a frequent complication in patients with malignancies, its treatment remains challenge daily practice. Here, we report the clinical course of 51-year-old woman presenting highly thrombogenic paraneoplastic coagulopathy. Despite therapeutic anticoagulation various agents, including rivaroxaban, fondaparinux, and low-molecular-weight heparin, patient suffered from recurrent venous arterial thromboembolism. Locally advanced endometrial cancer was...
421 Background: Characteristics and outcome of non-seminomatous testicular germ cell tumours (NSGCT) with teratoma-containing primaries are still under debate. Methods: We performed a retrospective analysis including 557 patients metastatic NSGCT as registry study within the "German Testicular Cancer Study Group". Results: Of eligible NSGCT, 237 (42%) all orchiectomy specimens had primaries, while 320 (58%) were teratoma-free. Teratoma-containing significantly higher clinical stage (p=0.002)...
Introduction Although cancer-associated thrombosis (CAT) is a frequent complication in patients with malignancies [1], its treatment remains challenging daily practice. Here, we report the clinical course of 51-year-old woman who developed highly thrombogenic paraneoplastic coagulopathy due to locally advanced endometrial cancer.
422 Background: As the characteristics and outcome of non-seminomatous testicular germ cell tumours (NSGCT) with teratoma-containing primaries are still under debate, this study aims at evaluating recurrence-free tumor-specific survival in cohort patients. Methods: We performed a retrospective analysis including 218 patients metastatic NSGCT as registry within "German Testicular Cancer Study Group". analysed patient well follow-up all included patients, being treated from 2000 to 2021....