A5014953176- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Estrogen and related hormone effects
- Probiotics and Fermented Foods
- Urinary Tract Infections Management
- Oral microbiology and periodontitis research
- Gut microbiota and health
- Trace Elements in Health
- Peptidase Inhibition and Analysis
- Vibrio bacteria research studies
- Malaria Research and Control
- Helicobacter pylori-related gastroenterology studies
- Bacterial biofilms and quorum sensing
- Nail Diseases and Treatments
- Ferrocene Chemistry and Applications
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Reproductive System and Pregnancy
- Antibiotics Pharmacokinetics and Efficacy
- Bioactive Compounds and Antitumor Agents
- Endoplasmic Reticulum Stress and Disease
University of California, Los Angeles
2024
Olive View-UCLA Medical Center
2024
The University of Queensland
2015-2022
Walter and Eliza Hall Institute of Medical Research
2020-2022
The University of Melbourne
2008-2021
Tarumanagara University
2021
VIB-VUB Center for Structural Biology
2010-2018
Vrije Universiteit Brussel
2013-2018
Griffith University
2015
ABSTRACT Urinary tract infection (UTI) is a disease of extremely high incidence in both community and nosocomial settings. UTIs cause significant morbidity mortality, with approximately 150 million cases globally per year. Uropathogenic Escherichia coli (UPEC) the primary UTI generally treated empirically. However, rapidly increasing caused by multidrug-resistant UPEC strains has led to limited available treatment options highlights urgent need develop alternative prevention strategies. In...
Significance Uropathogenic Escherichia coli (UPEC) is responsible for most urinary tract infections and also a frequent cause of sepsis, thus necessitating an understanding UPEC-mediated subversion innate immunity. The role zinc in the immune response against UPEC infection, whether this pathogen counters response, has not been examined. Here we demonstrate, both vitro vivo, that evades resists immune-mediated toxicity to persist disseminate within host. Moreover, have defined set genes...
ABSTRACT Porphyromonas gingivalis is an anaerobic, asaccharolytic, gram-negative bacterium that has essential requirements for both iron and protoporphyrin IX, which it preferentially obtains as heme. A combination of large-scale quantitative proteomic analysis using stable isotope labeling strategies mass spectrometry, together with transcriptomic custom-made DNA microarrays, was used to identify changes in P. W50 protein transcript abundances on changing from heme-excess heme-limited...
Abstract Background Porphyromonas gingivalis in subgingival dental plaque, as part of a mature biofilm, has been strongly implicated the onset and progression chronic periodontitis. In this study using DNA microarray we compared global gene expression P. biofilm with that its planktonic counterpart grown same continuous culture. Results Approximately 18% (377 genes, at 1.5 fold or more, P -value < 0.01) genome was differentially expressed when bacterium biofilm. Genes were down-regulated...
ABSTRACT Porphyromonas gingivalis , a periodontal pathogen, expresses group of surface proteins with common C-terminal domain (CTD) that are exported by novel secretion system to the surface, where they covalently attached. Using RgpB as model CTD protein, we have produced series site-directed mutations in sequence at conserved residues and may be modified and, hence, The mutant were expressed P. host lacking functional RgpA Arg-specific proteases. mutants Y674F, Y674F Y718F, T675Q S679Q...
ObjectivesTo identify and to characterize small-molecule inhibitors that target the subunit polymerization of type 1 pilus assembly in uropathogenic Escherichia coli (UPEC).
Curli are bacterial surface-associated amyloid fibers that bind to the dye Congo red (CR) and facilitate uropathogenic
ABSTRACT Carbapenem-resistant Enterobacteriaceae are urgent threats to global human health. These organisms produce β-lactamases with carbapenemase activity, such as the metallo-β-lactamase NDM-1, which is notable due its association mobile genetic elements and lack of a clinically useful inhibitor. Here we examined ability copper inhibit activity NDM-1 explored potential coordination complex mechanism efficiently deliver an adjuvant in clinical therapeutics. An NDM-positive Escherichia coli...
E. coli is a multifaceted pathogen of major significance to global human health and an important contributor increasing antibiotic resistance. Given the paucity therapies still effective against multidrug-resistant pathogenic strains, novel treatment prevention strategies are urgently required. In this study, we defined core accessory components genome by examining large collection draft completely sequenced strains available from public databases. This data set was mined employing...
ABSTRACT The vacuolating autotransporter toxin (Vat) contributes to uropathogenic Escherichia coli (UPEC) fitness during systemic infection. Here, we characterized Vat and investigated its regulation in UPEC. We assessed the prevalence of vat a collection 45 UPEC urosepsis strains showed that it was present 31 (68%) isolates. isolates containing gene corresponded three major E. sequence types (ST12, ST73, ST95), these secreted protein. Further analysis genomic locus identified conserved...
Uropathogenic Escherichia coli (UPEC) is the cause of ~75% all urinary tract infections (UTIs) and increasingly associated with multidrug resistance. This includes UPEC strains from recently emerged globally disseminated sequence type 131 (ST131), which now dominant fluoroquinolone-resistant clone worldwide. Most ST131 are motile produce H4-type flagella. Here, we applied a combination saturated Tn5 mutagenesis transposon directed insertion site sequencing (TraDIS) as high throughput genetic...
Abstract Escherichia coli sequence type 131 (ST131) is a globally dominant multidrug resistant clone associated with urinary tract and bloodstream infections. Most ST131 strains exhibit resistance to multiple antibiotics cause infections limited treatment options. The largest sub-clonal lineage fluoroquinolones, contains the 1 fimbriae fimH 30 allele expresses an H4 flagella antigen. Flagella are motility organelles that contribute UPEC colonisation of upper tract. In this study, we examined...
Autotransporters are the largest family of outer membrane and secreted proteins in Gram-negative bacteria. Most autotransporters localised to bacterial surface where they promote colonisation host epithelial surfaces. Here we present crystal structure UpaB, an autotransporter that is known contribute uropathogenic E. coli (UPEC) urinary tract. We provide evidence UpaB can interact with glycosaminoglycans fibronectin. Unique modifications its core β-helical create a groove on one side protein...
Shiga toxin Stx2e is the major known agent that causes edema disease in newly weaned pigs. This severe characterized by neurological disorders, hemorrhagic lesions, and frequent fatal outcomes. consists of an enzymatically active A subunit five B subunits bind to a specific glycolipid receptor on host cells. It evident antibodies binding or variants may have capability inhibit their cytotoxicity. Here, we report discovery characterization VHH single domain antibody (nanobody) isolated from...
Bacterial autotransporters comprise a C-terminal β-barrel domain, which must be correctly folded and inserted into the outer membrane to facilitate translocation of N-terminal passenger domain cell exterior. Once at surface, domains most are an elongated β-helix. In cellular context, key molecules catalyze assembly autotransporter domain. However, how folds its functional form is poorly understood. Here we use mutational analysis on Pet show that β-hairpin structure fifth extracellular loop...
The human stomach pathogen Helicobacter pyloriattaches to healthy and inflamed gastric tissue through members of a paralogous family 'Helicobacter outer membrane proteins' (Hops), including adhesins BabA, SabA, HopQ, LabA HopZ. Hops share conserved 25 kDa C-terminal region that is thought form an autotransporter-like transmembrane domain. Instead, our results show contain non-continuous domain, composed seven predicted β-strands at the C-terminus one N-terminus. Folding localization β-domain...
Extra-intestinal pathogenic Escherichia coli (ExPEC) belong to a critical priority group of antibiotic resistant pathogens. ExPEC establish gut reservoirs that seed infection the urinary tract and bloodstream, but mechanisms colonisation remain be properly understood. Ucl fimbriae are attachment organelles facilitate adherence. Here, we investigated cellular receptors for expression define molecular Ucl-mediated gut. We demonstrate differential in sequence types associated with disseminated...
Abstract Copper resistance is a key virulence trait of the uropathogen Proteus mirabilis . Here we show that P. ScsC (PmScsC) contributes to this defence mechanism by enabling swarming in presence copper. We also demonstrate PmScsC thioredoxin-like disulfide isomerase but, unlike other characterized proteins family, it trimeric. trimerization and its active site cysteine are required for wild-type activity Moreover, exhibits unprecedented motion as consequence shape-shifting motif linking...
Uropathogenic Escherichia coli (UPEC) of sequence type 131 (ST131) are a pandemic multidrug resistant clone associated with urinary tract and bloodstream infections. Type 1 fimbriae, major UPEC virulence factor, essential for ST131 bladder colonization. The globally dominant sub-lineage strains, clade C/H30-R, possess an ISEc55 insertion in the fimB gene that controls phase-variable fimbriae expression via invertible fimS promoter. We report inactivation these strains causes altered...