A5075969337- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Salmonella and Campylobacter epidemiology
- Tuberculosis Research and Epidemiology
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Infective Endocarditis Diagnosis and Management
- Urinary Tract Infections Management
- Aquaculture disease management and microbiota
- Pneumonia and Respiratory Infections
- Gut microbiota and health
- Microbial infections and disease research
- Burkholderia infections and melioidosis
- Plant Pathogenic Bacteria Studies
- Cystic Fibrosis Research Advances
Griffith University
2020-2022
Charles Darwin University
2022
Menzies School of Health Research
2022
The University of Queensland
2012-2021
Royal Brisbane and Women's Hospital
2009-2018
Queensland University of Technology
2011
Prince Charles Hospital
2011
University of Pittsburgh Medical Center
2008-2010
Princess Alexandra Hospital
2010
University of Pittsburgh
2008-2009
Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. clone O25b:H4-ST131 has recently emerged globally a leading multi-drug resistant pathogen and bloodstream infections in hospitals the community. While most molecular studies date examine mechanisms conferring resistance ST131, relatively little is known about their virulence potential. Here we examined ST131 clinical isolates from two geographically diverse...
The molecular epidemiology and mechanisms of resistance carbapenem-resistant Enterobacteriaceae (CRE) were determined in hospitals the countries Gulf Cooperation Council (GCC), namely, Saudi Arabia, United Arab Emirates, Oman, Qatar, Bahrain, Kuwait. Isolates subjected to PCR-based detection antibiotic-resistant genes repetitive sequence-based PCR (rep-PCR) assessments clonality. Sixty-two isolates which screened positive for potential carbapenemase production assessed, 45 found produce...
ABSTRACT A total of 49 unique clinical isolates multidrug-resistant (MDR) Acinetobacter baumannii identified at a tertiary medical center in Pittsburgh, Pennsylvania, between August 2006 and September 2007 were studied for the genetic basis their MDR phenotype. Approximately half all A. during this period qualified as MDR, defined by nonsusceptibility to three or more antimicrobials routinely tested microbiology laboratory. Among isolates, 18.4% resistant imipenem. The frequencies resistance...
The public health threat posed by a looming 'post-antibiotic' era necessitates new approaches to antibiotic discovery. Drug development has typically avoided exploitation of membrane-binding properties, in contrast nature's control biological pathways via modulation membrane-associated proteins and membrane lipid composition. Here, we describe the rejuvenation glycopeptide vancomycin selective targeting bacterial membranes. Peptide libraries based on positively charged electrostatic effector...
(See editorial commentary by Bronomo, on pages 485–487.) Carbapenem resistance in Klebsiella pneumoniae is most notably due to the K. carbapenemase (KPC) β-lactamase. In this report, we describe occurrence of a newly described mechanism carbapenem resistance, NDM-1 β-lactamase, patient who received medical attention (but was not hospitalized) India.
Background. Transrectal ultrasound–guided (TRUS) prostate biopsy is a commonly performed procedure, and fluoroquinolones are the most frequently given prophylactic antimicrobials. In context of increasing fluoroquinolone resistance, international emergence fluoroquinolone-resistant sequence type 131 (ST131) Escherichia coli, we describe large series E. coli bacteremia after TRUS biopsy.
The molecular epidemiology and mechanisms of resistance carbapenem-resistant Acinetobacter baumannii (CRAB) were determined in hospitals the states Cooperation Council for Arab States Gulf (Gulf [GCC]), namely, Saudi Arabia, United Emirates, Oman, Qatar, Bahrain, Kuwait. Isolates subjected to PCR-based detection antibiotic genes repetitive sequence-based PCR (rep-PCR) assessments clonality. Selected isolates multilocus sequence typing (MLST). We investigated 117 resistant carbapenem...
Long-term care facilities (LTCFs) are a potentially important reservoir of multidrug-resistant (MDR) organisms; however, limited data exist. A point-prevalence study was conducted in four co-located LTCFs Australia. Nasal and rectal swabs were cultured for methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE) MDR Gram-negative bacilli (GNB). Molecular typing resistance detection performed. Risk factors colonization with an organism determined using nested...
ABSTRACT The use of combination antibiotic therapy may be beneficial against rapidly emerging resistance in Pseudomonas aeruginosa . aim this study was to systematically investigate vitro bacterial killing and emergence with colistin alone imipenem multidrug-resistant (MDR) P. Time-kill studies were conducted over 48 h using 5 clinical isolates ATCC 27853 at two inocula (∼10 6 ∼10 8 CFU/ml); MDR, non-MDR, colistin-heteroresistant -resistant strains included. Nine colistin-imipenem...
ABSTRACT Combination therapy may be required for multidrug-resistant (MDR) Pseudomonas aeruginosa . The aim of this study was to systematically investigate bacterial killing and emergence colistin resistance with doripenem combinations against MDR P. Studies were conducted in a one-compartment vitro pharmacokinetic/pharmacodynamic model 96 h at two inocula (∼10 6 ∼10 8 CFU/ml) colistin-heteroresistant reference strain (ATCC 27853) colistin-resistant clinical isolate (19147 n/m). Four...
Mycobacterium abscessus is a rapidly growing mycobacterium responsible for progressive pulmonary disease, soft tissue and wound infections. The incidence of disease due to M. has been increasing in Queensland. In study Brisbane drinking water, was isolated from ten different locations.The aim this compare genotypically the isolates obtained water those human clinical specimens.Between 2007 2009, eleven confirmed as were recovered potable one strain rainwater tank another swimming pool two...
ABSTRACT A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type β-lactamases. When combined with nonsusceptibility to ertapenem, easy perform reliably differentiated isolates producing KPC-type β-lactamases from those other types
A combination of phenotypic and genotypic methods was used to investigate 70 unique Escherichia coli clinical isolates identified as producing extended-spectrum beta-lactamases (ESBLs) at a medical center in Pittsburgh, PA, between 2007 2008. Fifty-seven (81%) produced CTX-M-type ESBLs, among which CTX-M-15 predominant (n = 46). Isolates CTX-M-2, -9, -14, -65 were also identified. One CTX-M-producing isolate coproduced CMY-2 cephalosporinase. Ten (14%) SHV-type either SHV-5 or SHV-7. Two...
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae, Escherichia coli, and Serratia marcescens were sequentially identified in a patient who underwent small bowel transplantation. Molecular typing plasmid analysis suggested that the KPC gene was acquired by E. most likely from subsequently transferred to S. marcescens.
ABSTRACT Acinetobacter baumannii is a multidrug-resistant pathogen associated with hospital outbreaks of infection across the globe, particularly in intensive care unit. The ability A. to survive environment for long periods linked antibiotic resistance and its capacity form biofilms. Here we studied prevalence, expression, function biofilm-associated protein (Bap) 24 carbapenem-resistant ST92 strains isolated from single institution over 10-year period. bap gene was highly prevalent, 22/24...
ABSTRACT The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) has been increasing worldwide. bla IMP reported to be the predominant carbapenemase-encoding gene within in Australia. However, there are limited data currently available on CPE from Queensland, A total 58 isolates were isolated between July 2009 and March 2014 Queensland hospitals. clonality was determined by Diversilab repetitive sequence-based PCR. investigated for resistance mechanisms carbapenemase,...
The New Delhi metallo-β-lactamase gene (bla(NDM-1)) has emerged as a worldwide concern among isolates of Enterobacteriaceae. Its epidemiology is been strongly associated with travel and healthcare on the Indian Subcontinent. We report two cases urinary tract infection Enterobacteriaceae harboring bla(NDM-1). Both presented in community-dwelling individuals Australia were to One isolate Escherichia coli harbored previously undescribed enzyme variant bla(NDM-3), differing from bla(NDM-1) by...
Combination therapy is an important option in the fight against Gram-negative 'superbugs'. This study systematically investigated bacterial killing and emergence of polymyxin resistance with B chloramphenicol combinations used New Delhi metallo-β-lactamase (NDM)-producing MDR Klebsiella pneumoniae. Four NDM-producing K. pneumoniae strains were employed. The presence genes conferring to was examined by PCR. Time–kill studies (inocula ∼106 cfu/mL) conducted using various clinically achievable...
ABSTRACT bla NDM has been reported in different Enterobacteriaceae species and on numerous plasmid replicon types (Inc). Plasmid typing, combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer spread antimicrobial resistance determinants between genetically unrelated hosts. The genetic context heterogeneous. In this study, we genomically characterized 12 NDM-producing isolated Australia 2012 2014: Escherichia coli ( n = 6), Klebsiella...
Carbapenem-resistant Enterobacteriaceae (CRE) represent an urgent threat to human health. Here we report the application of several complementary whole-genome sequencing (WGS) technologies characterise a hospital outbreak blaIMP-4 carbapenemase-producing E. hormaechei. Using Illumina sequencing, determined that all strains were sequence type 90 (ST90) and near-identical. Comparison publicly available data linked isolates 2013 isolate from same ward, suggesting environmental source in...