A5052014544- Escherichia coli research studies
- Urinary Tract Infections Management
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Urinary Bladder and Prostate Research
- Vibrio bacteria research studies
- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- Bladder and Urothelial Cancer Treatments
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Bacterial Genetics and Biotechnology
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Pediatric Urology and Nephrology Studies
- Virus-based gene therapy research
- Infectious Disease Case Reports and Treatments
- Probiotics and Fermented Foods
- Toxin Mechanisms and Immunotoxins
- RNA modifications and cancer
- Bacteriophages and microbial interactions
University of Utah
2015-2024
North Medical Center
2020
Salt Lake Regional Medical Center
2017
Bipar
2006-2008
New York University
1999-2007
Washington University in St. Louis
1997-2002
Czech Academy of Sciences, Institute of Microbiology
2001
The University of Texas at Austin
1994-1997
Swedish University of Agricultural Sciences
1997
Virtually all uropathogenic strains of Escherichia coli encode filamentous surface adhesive organelles called type 1 pili. High-resolution electron microscopy infected mouse bladders revealed that pilus tips interacted directly with the lumenal bladder, which is embedded hexagonal arrays integral membrane glycoproteins known as uroplakins. Attached pili were shortened and facilitated intimate contact bacteria uroplakin-coated host cells. Bacterial attachment resulted in exfoliation bladder...
ABSTRACT The vast majority of urinary tract infections are caused by strains uropathogenic Escherichia coli that encode filamentous adhesive organelles called type 1 pili. These structures mediate both bacterial attachment to and invasion bladder epithelial cells. However, the mechanism which pilus-mediated contributes pathogenesis a infection is unknown. Here we show 1-piliated uropathogens can invade superficial cells line lumenal surface subsequently replicate, forming massive foci...
Numerous antibiotics have proven to be effective at ameliorating the clinical symptoms of urinary tract infections (UTIs), but recurrent and chronic continue plague many individuals. Most UTIs are caused by strains uropathogenic Escherichia coli (UPEC), which can form both extra- intracellular biofilm-like communities within bladder. UPEC also persist inside host urothelial cells in a more quiescent state, sequestered late endosomal compartments. Here, we tested panel 17 different...
Uropathogenic Escherichia coli (UPEC), the primary causative agent of urinary tract infections, typically express filamentous adhesive organelles called type 1 pili that mediate both bacterial attachment to and invasion bladder urothelial cells. Several host proteins have previously been identified as receptors for pili, but none conclusively shown promote UPEC entry into Using overlay assays with FimH, purified pilus adhesin, mass spectroscopy, we beta1 alpha3 integrins key UPEC. FimH...
No AccessJournal of UrologyUrological Survey: Abstracts: Urinary Tract Infection1 Apr 1999Induction and Evasion Host Defenses by Type 1-Piliated Uropathogenic Escherichia Coli M.A. Mulvey, Y.S. Lopez-Boado, C.L. Wilson, R. Roth, W.C. Parks, J. Heuser, S.J. Hultgren MulveyM.A. Mulvey More articles this author , Lopez-BoadoY.S. Lopez-Boado WilsonC.L. Wilson RothR. Roth ParksW.C. Parks HeuserJ. Heuser HultgrenS.J. View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)61739-7AboutFull...
Abstract One mechanism of initiating innate host defenses against uropathogenic Escherichia coli (UPEC) is the production cytokines by bladder epithelial cells; however, means which these cells recognize bacterial pathogens poorly understood. Type 1 pili, expressed majority UPEC, have been shown to a critical role in inducing expression IL-6 after exposure E. coli. In this study, we demonstrate that type pili are not sufficient activate cells. Instead, it was invasion mediated augments...
ABSTRACT Upon activation by double-stranded RNA in virus-infected cells, the cellular PKR kinase phosphorylates translation initiation factor eukaryotic 2 (eIF2) and thereby inhibits protein synthesis. The γ34.5 Us11 gene products encoded herpes simplex virus type 1 (HSV-1) are dedicated to preventing accumulation of phosphorylated eIF2. While specifies a regulatory subunit for phosphatase 1α, encodes an binding that also prevents activation. mutants fail grow on variety human cells as eIF2...
Uropathogenic Escherichia coli (UPEC), the principal cause of urinary tract infection in women, attaches to superficial facet cell layer bladder epithelium (urothelium) via its FimH adhesin. Attachment triggers exfoliation bacteria-laden cells, followed by rapid reconstitution urothelium through differentiation underlying basal and intermediate cells. We have used DNA microarrays define molecular regulators urothelial renewal host defense expressed adult C57Bl/6 female mice during early...
Toxin-antitoxin (TA) systems are prevalent in many bacterial genomes and have been implicated biofilm persister cell formation, but the contribution of individual chromosomally encoded TA during pathogenesis is not well understood. Of known by Escherichia coli, only a subset associated with strains extraintestinal pathogenic E. coli (ExPEC). These pathogens colonize diverse niches major cause sepsis, meningitis, urinary tract infections. Using murine infection model, we show that two...
ABSTRACT Hfq is a bacterial RNA chaperone involved in the posttranscriptional regulation of many stress-inducible genes via small noncoding RNAs. Here, we show that critical for uropathogenic Escherichia coli (UPEC) isolate UTI89 to effectively colonize bladder and kidneys murine urinary tract infection model system. The disruption hfq did not affect adherence or invasion host cells but limit development intracellular microcolonies by within terminally differentiated epithelial line lumen...
Abstract Recurrent urinary tract infections (rUTIs) are extremely common, with ~ 25% of all women experiencing a recurrence within 1 year their original infection. Escherichia coli ST131 is globally dominant multidrug resistant clone associated high rates rUTI. Here, we show the dynamics an population over 5-year period from one elderly woman rUTI since 1970s. Using whole genome sequencing, identify indigenous clonal lineage (P1A) linked to and persistence in fecal flora, providing...
The gram-negative bacterium Escherichia coli is the leading cause of urinary tract infection. interaction between type 1 piliated E. and bladder epithelial cells leads to rapid production inflammatory mediators, such as interleukin-6 (IL-6) IL-8. Conflicting reports have been published in literature regarding mechanism by which uroepithelial are activated coli. In particular, role lipopolysaccharide (LPS) these responses has an area significant debate. Much data arguing against LPS-mediated...
Strains of uropathogenic Escherichia coli (UPEC) can invade terminally differentiated superficial bladder epithelial cells and subsequently multiply, forming large biofilm-like inclusions referred to as pods. In contrast, within immature UPEC enter a more quiescent state often fail replicate appreciably. As undergo terminal differentiation the actin cytoskeleton is radically diminished, phenomenon that we reasoned could influence intracellular fate UPEC. Here show undifferentiated trafficked...
In the efforts of viruses to dominate and control critical cellular pathways, generate considerable intracellular stress within their hosts. particular, capacity resident endoplasmic reticulum (ER) chaperones properly process acute increase in client protein load is significantly challenged. Such alterations typically induce unfolded response, one component which acts through IRE1 restore ER homeostasis by expanding folding capabilities, whereas other arm activates eIF-2alpha (alpha subunit...
Uropathogenic Escherichia coli (UPEC) are the major cause of urinary tract infections (UTIs), and they have capacity to induce death exfoliation target uroepithelial cells. This process can be facilitated by pore-forming toxin α-hemolysin (HlyA), which is expressed secreted many UPEC isolates. Here, we demonstrate that HlyA potently inhibit activation Akt (protein kinase B), a key regulator host cell survival, inflammatory responses, proliferation, metabolism. ablates via an extracellular...
Strains of uropathogenic Escherichia coli (UPEC) are the primary cause urinary tract infections, representing one most widespread and successful groups pathogens on planet. To colonize persist within tract, UPEC must be able to sense respond appropriately environmental stresses, many which can compromise bacterial envelope. The Cpx two-component envelope stress response system is comprised inner membrane histidine kinase CpxA, cytosolic regulator CpxR, periplasmic auxiliary factor CpxP....