A5081951575- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Protein Structure and Dynamics
- Protein Kinase Regulation and GTPase Signaling
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Vibrio bacteria research studies
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- Legionella and Acanthamoeba research
- Helicobacter pylori-related gastroenterology studies
- Peptidase Inhibition and Analysis
- Chemical Synthesis and Analysis
- Cellular transport and secretion
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Glycosylation and Glycoproteins Research
- thermodynamics and calorimetric analyses
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- Polysaccharides and Plant Cell Walls
Birkbeck, University of London
2014-2025
Institute of Structural and Molecular Biology
2016-2025
University College London
2016-2025
UCL Australia
2012-2013
Utrecht University
2011
The Wistar Institute
2009
Universitat Pompeu Fabra
2008
University of London
2008
Barcelona Biomedical Research Park
2008
European Synchrotron Radiation Facility
2005
The BCL-2 family of proteins is composed both pro- and antiapoptotic regulators, although its most critical biochemical functions remain uncertain. structural similarity between the BCL-X L monomer several ion-pore-forming bacterial toxins has prompted electrophysiologic studies. Both BAX insert into KCl-loaded vesicles in a pH-dependent fashion demonstrate macroscopic ion efflux. Release maximum at ≈pH 4.0 for proteins; however, demonstrates broader pH range activity. purified also planar...
Many Gram-negative pathogens assemble architecturally and functionally diverse adhesive pili on their surfaces by the chaperone-usher pathway. Immunoglobulin-like periplasmic chaperones escort pilus subunits to usher, a large protein complex that facilitates translocation assembly of across outer membrane. The crystal structure PapD-PapK chaperone-subunit complex, determined at 2.4 angstrom resolution, reveals chaperone functions donating its G(1) beta strand complete immunoglobulin-like...
The first step in the colonization of human urinary tract by pathogenic Escherichia coli is mannose-sensitive binding FimH, adhesin present at tip type 1 pili, to bladder epithelium. We elucidated crystallographically interactions FimH with D-mannose. unique site pocket occupied D-mannose was probed using site-directed mutagenesis. All but one mutants examined had greatly diminished mannose-binding activity and also lost ability bind cells. mono-saccharide delineated from this mannotriose...
The maleimide motif is widely used for the selective chemical modification of cysteine residues in proteins. Despite widespread utilization, there are some potential limitations, including irreversible nature reaction and, hence, and number attachment positions. We conceived a new class which would address these limitations provide opportunities protein modification. report herein use mono- dibromomaleimides reversible illustrate this on SH2 domain Grb2 adaptor (L111C). After initial with...
Type IV secretion systems (T4SSs) are important virulence factors used by Gram-negative bacterial pathogens to inject effectors into host cells or spread plasmids harboring antibiotic resistance genes. We report the 15 angstrom resolution cryo–electron microscopy structure of core complex a T4SS. The is composed three proteins, each present in 14 copies and forming ∼1.1-megadalton two-chambered, double membrane–spanning channel. double-walled, with component apparently spanning large part...
Bacterial conjugation is the fundamental process of unidirectional transfer DNAs, often plasmid from a donor cell to recipient cell1. It primary means by which antibiotic resistance genes spread among bacterial populations2,3. In Gram-negative bacteria, mediated large transport apparatus-the conjugative type IV secretion system (T4SS)-produced and embedded in both its outer inner membranes. The T4SS also elaborates long extracellular filament-the pilus-that essential for DNA transfer4,5....
BAD interacts with anti-apoptotic molecules BCL-2 and BCL-X<sub>L</sub> promotes apoptosis. is phosphorylated on serine residues in response to a survival factor, interleukin-3. Phosphorylated cannot bind or at membrane sites found the cytosol bound 14-3-3. We report here that deletion mapping site-directed mutagenesis identified BH3 domain within proved necessary for both its heterodimerization death agonist activity. Substitution of conserved Leu<sup>151</sup> Ala amphipathic α-helix...
A chemical synthesis platform with broad applications and flexibility was rationally designed to inhibit biogenesis of adhesive pili assembled by the chaperone–usher pathway in Gram-negative pathogens. The activity a family bicyclic 2-pyridones, termed pilicides, evaluated two different pilus systems uropathogenic Escherichia coli . Hemagglutination mediated either type 1 or P pili, adherence bladder cells, biofilm formation were all reduced ≈90% laboratory clinical E. strains. structure...