A5100678593- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Platelet Disorders and Treatments
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Blood Coagulation and Thrombosis Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Trauma and Emergency Care Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood disorders and treatments
- Phagocytosis and Immune Regulation
- Atherosclerosis and Cardiovascular Diseases
- Blood transfusion and management
- Iron Metabolism and Disorders
- MicroRNA in disease regulation
- Blood properties and coagulation
- Ion channel regulation and function
- HIV Research and Treatment
- Coronary Interventions and Diagnostics
- Adenosine and Purinergic Signaling
- Acute Myeloid Leukemia Research
- Kruppel-like factors research
- Abdominal Trauma and Injuries
University of Washington
2012-2024
Bloodworks Northwest
2015-2024
Hospital Universitario Puerta del Mar
2024
University of Washington Medical Center
2021
Oregon Health & Science University
2020
Seattle University
2013-2014
Instituto de Biotecnología de León
2014
Probelte Pharma (Spain)
2013
GTx (United States)
2009
Baylor College of Medicine
2002-2007
Microvascular networks support metabolic activity and define microenvironmental conditions within tissues in health pathology. Recapitulation of functional microvascular structures vitro could provide a platform for the study complex vascular phenomena, including angiogenesis thrombosis. We have engineered living three-dimensional tissue scaffolds demonstrated their biofunctionality vitro. describe lithographic technique used to form endothelialized microfluidic vessels native collagen...
Thrombotic thrombocytopenic purpura (TTP) is a life-threatening disease characterized by systemic microvascular thrombosis caused adhesion of platelets to ultra-large vWF (ULVWF) multimers. These multimers accumulate because deficiency the processing enzyme ADAMTS13. protein forms long from homodimers that first form through C-terminal disulfide bonds and then join their N termini further bonding. N-acetylcysteine (NAC) an FDA-approved drug has been used treat chronic obstructive lung...
Thrombotic thrombocytopenic purpura is caused by congenital or acquired deficiency of ADAMTS-13, a metalloprotease that cleaves the endothelium-derived ultra-large multimers von Willebrand factor (ULVWF). The proteolysis converts hyper-reactive and thrombogenic ULVWF into smaller less adhesive plasma forms. Activity ADAMTS-13 usually measured in static system under non-physiological conditions require protein denaturation prolonged incubation. We have demonstrated previously multimers, upon...
Summary Vascular occlusion, thromboembolism and strokes are hallmark events in sickle cell disease (SCD). The von Willebrand factor (VWF), largest adhesive protein circulation, has been implicated as major component these processes. In SCD, a high level of extracellular haemoglobin (Hb) plasma shown parallely associated with the pathogenesis. Investigating effect Hb we observed that purified significantly inhibited ADAMTS-13 cleavage VWF under static flow conditions. bound potently to...
Significance The myeloproliferative neoplasms (MPNs) are a group of hematological malignancies characterized by increased numbers myeloid blood cells, such as platelets, erythrocytes, and neutrophils. main causes illness death in patients with MPNs arterial venous clotting also, conversely, bleeding complications. However, the these conditions poorly understood. In this paper, we use mouse model to determine cell types responsible for abnormal MPNs. We demonstrate that endothelial type lines...
Stimulation of monocytes with P-selectin induces the synthesis an array mediators inflammation, as well expression tissue factor (TF), main initiator coagulation. Because membrane-bound reactions coagulation are profoundly influenced by presence phosphatidylserine on membranes cells, factors that increase its may have impact coagulation.Using flow cytometry, we studied effect in blood and monocytic THP-1. Soluble at biologically relevant concentrations (0.31 to 2.5 microg/mL) induced a...
Thrombocytopenia is one of the complications for in vivo administration adenovirus serotype 5 (Ad5) vectors after intravenous injection. In this paper, we investigated mechanism Ad5-induced thrombocytopenia and how these effects are attenuated by polyethylene glycol (PEG) modification Ad5 (Ad-PEG). After injection, accelerated platelet loss was observed Ad-injected mice but not their Ad-PEG-injected counterparts. This induced corresponded with increases coagulation D-dimer levels,...
Abstract We developed a high-throughput mass spectrometry–based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay analyze plasma from patients with known oxidative stress (sickle cell disease sepsis) patient sickle treated the antioxidant N-acetylcysteine suggests that cysteine disulfides, particular protein-bound cysteine, serve as sensitive biomarkers for extent effectiveness treatment.
Abstract Microvascular thrombosis in patients with thrombotic thrombocytopenic purpura (TTP) is initiated by GPIbα-mediated platelet binding to von Willebrand factor (VWF). Binding of VWF GPIbα causes activation the surface integrin αIIbβ3. However, mechanism GPIbα-initiated αIIbβ3 and its clinical importance for microvascular remain elusive. Deletion C-type lectin-like receptor 2 (CLEC-2) did not prevent platelets but specifically inhibited aggregation induced mice. CLEC-2 also GPIbα. Using...
Transplantation-associated thrombotic microangiopathy (TA-TMA) is a complication of allogeneic hematopoietic cell transplantation (HCT) that often occurs following the development acute graft-versus-host disease (aGVHD). In this study, we aimed to identify early TMA biomarkers among patients with aGVHD. We performed nested-case-control study from prospective cohort HCT recipients, matching on timing and severity antecedent identified 13 cases 25 non-TMA controls 208 in cohort. Using...