A5001664596- Platelet Disorders and Treatments
- Complement system in diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Renal Diseases and Glomerulopathies
- COVID-19 epidemiological studies
- Acute Ischemic Stroke Management
- COVID-19 Clinical Research Studies
- Signaling Pathways in Disease
- Blood groups and transfusion
- Mechanical Circulatory Support Devices
- SARS-CoV-2 and COVID-19 Research
- Transplantation: Methods and Outcomes
- Trypanosoma species research and implications
- Antiplatelet Therapy and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Apelin-related biomedical research
- Neuroinflammation and Neurodegeneration Mechanisms
- Adenosine and Purinergic Signaling
- Seaweed-derived Bioactive Compounds
- Hemophilia Treatment and Research
- Blood properties and coagulation
- HIV/AIDS Impact and Responses
KU Leuven
2014-2025
University College West Flanders
2023
Research Institute for Chromatography
2009-2023
UCLouvain
2022
Platelet recruitment to sites of blood vessel damage is highly dependent upon von Willebrand factor (VWF). VWF platelet-tethering function proteolytically regulated by the metalloprotease ADAMTS13. Proteolysis depends shear-induced conformational changes in that reveal A2 domain cleavage site. Multiple ADAMTS13 exosite interactions are involved recognition unfolded domain. Here we report through kinetic analyses that, binding VWF, cysteine-rich and spacer exosites bring enzyme substrate into...
BACKGROUND: In ischemic stroke due to large vessel occlusion, cerebral blood flow is impaired by thromboemboli, which can originate from various sources in the body. A better understanding of thromboembolus composition improve our underlying pathophysiology and potentially guide improvement prevention strategies. The aim this study therefore was perform a large-sample multi-parameter quantitative histological analysis retrieved thromboemboli patients. METHODS: Thromboemboli (n=501) were...
Abstract Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is characterized by severe ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeats, member 13) deficiency, the presence of anti-ADAMTS13 autoantibodies an open conformation a cryptic epitope in spacer domain exposed. A detailed knowledge will help identifying pathogenic antibodies elucidating cause deficiency. We aimed at cloning from iTTP patients to study their epitopes inhibitory characteristics....
Autoantibodies are key biomarkers in clinical diagnosis of autoimmune diseases routinely detected by enzyme-linked immunosorbent assays (ELISAs). However, the complexity these is limiting their use routine diagnostics. Fiber optic-surface plasmon resonance (FO-SPR) can overcome limitations, but improved surface chemistries still needed to guarantee detection autoantibodies complex matrices. In this paper, we describe development an FO-SPR immunoassay for plasma samples from immune-mediated...
von Willebrand factor (VWF) plays an important role in ischemic stroke. However, the exact mechanism by which VWF mediates progression of stroke brain damage is not completely understood. Using flow cytometric analysis single cell suspensions prepared from tissue and immunohistochemistry, we investigated potential inflammatory mechanisms contributes to a mouse model cerebral ischemia/reperfusion injury. Twenty-four hours after stroke, revealed that overall white blood recruitment...
Congenital thrombotic thrombocytopenic purpura (TTP) is characterized by mutations in the ADAMTS13 gene, which either impair protein secretion or influence (A Disintegrin-like And Metalloprotease domain with ThromboSpondin type-1 motif, member 13) activity. Phenotypic consequences of these have not yet been evaluated animal models for TTP.To identify vitro effect a novel mutation and to investigate whether this induces TTP vivo.All 29 exons exon-intron boundaries patient pregnancy-onset were...
In this study, we investigated a case of pregnancy-onset thrombotic thrombocytopenic purpura (TTP). The patient had severely decreased ADAMTS13 ( ad isintegrin nd m etalloprotease with t hrombo s pondin type 1 motif, member 13) activity levels during acute phase and the presence inhibitory anti-ADAMTS13 autoantibodies was demonstrated, which led to diagnosis immune-mediated TTP. However, only mildly restored remission, although antibodies were no longer detected. We hypothesized that genetic...
Thrombotic thrombocytopenic purpura (TTP) is a life-threatening thrombotic microangiopathy linked to deficiency in the metalloprotease ADAMTS13. In current study, novel mouse model for acquired TTP was generated facilitate development and validation of new therapies this disease. Therefore, large panel (n = 19) anti-mouse ADAMTS13 (mADAMTS13) monoclonal antibodies (mAbs) origin generated. Inhibitory anti-mADAMTS13 mAbs were identified using FRETS-VWF73 assay. Four strongly inhibited...
To mitigate the massive COVID-19 burden caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), several vaccination campaigns were initiated. We performed a single-center observational trial to monitor mid- (3 months) and long-term (10 adaptive immune response document breakthrough infections (BTI) in healthcare workers (n = 84) upon BNT162b2 real-world setting. Firstly, serology was determined through immunoassays. Secondly, antibody functionality analyzed via vitro binding...
Abstract Introduction Bleeding is the most frequent adverse event in patients with continuous flow mechanical circulatory support (CF-MCS) and has been linked to occurrence of acquired von Willebrand syndrome (aVWS). MCS devices cause an increased shear-induced proteolysis factor (VWF) by ADAMTS13, leading aVWS. Hence, specifically blocking ADAMTS13 might be efficient way rescue loss HMW VWF multimers CF-MCS patients. Purpose To investigate if using in-house developed inhibitory...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of ongoing COVID-19 pandemic. To prevent massive burden, several vaccination campaigns were initiated. We performed a single center observational trial to evaluate adaptive immunity in naive healthcare workers upon BNT162b2 vaccination. Methods Serological analysis was through conventional immunoassays. Antibody functionality analyzed via vitro neutralization assays. Circulating receptor-binding...
In recent years, candidate genes and proteins implicated in platelet function have been identified by various genomic approaches. To elucidate their exact role, we aimed to develop a method apply miRNA interference progenitor cells using GPIbα as proof-of-concept target protein. After silico vitro screening of siRNAs targeting (siGPIBAs), developed artificial miRNAs (miGPIBAs), which were tested CHO stably expressing GPIb-IX complex megakaryoblastic DAMI cells. Introduction siGPIBAs resulted...