A5086939300- Platelet Disorders and Treatments
- Complement system in diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood transfusion and management
- Blood groups and transfusion
- Blood Coagulation and Thrombosis Mechanisms
- Blood donation and transfusion practices
- SARS-CoV-2 and COVID-19 Research
- Blood properties and coagulation
- Antiplatelet Therapy and Cardiovascular Diseases
- Renal Diseases and Glomerulopathies
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Venous Thromboembolism Diagnosis and Management
- Peptidase Inhibition and Analysis
- Iron Metabolism and Disorders
- Erythrocyte Function and Pathophysiology
- Heparin-Induced Thrombocytopenia and Thrombosis
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Hemophilia Treatment and Research
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- Neurogenetic and Muscular Disorders Research
- Mechanical Circulatory Support Devices
- Monoclonal and Polyclonal Antibodies Research
Ghent University
2017-2024
CrossLang (Belgium)
2015-2023
Ghent University Hospital
2013-2023
Belgian Road Research Centre
2013-2021
KU Leuven
2005-2015
Rode Kruis-Vlaanderen
2014
Research Institute for Chromatography
2005-2012
Washington University in St. Louis
2009
Baruch College
2007
Inserm
2005
From 20 to 50% of patients who survive an acute episode the acquired form thrombotic thrombocytopenic purpura relapse but clinical and laboratory markers recurrence are not well established.In 109 enrolled in international registry we evaluated, frame a retrospective cohort study, predictive role metalloprotease ADAMTS13 as measured plasma during remission. Anti-ADAMTS13 antibodies von Willebrand factor were also evaluated smaller number same patients.Median values activity antigen...
Objective: The inability to regulate the inflammatory response initiated upon infection leads severe sepsis, characterized by widespread microvascular injury and thrombosis, organ ischemia, dysfunction. A disintegrin-like metalloprotease with thrombospondin type 1 repeats (ADAMTS)-13 regulates primary hemostasis proteolyzing von Willebrand factor (VWF). Decreased ADAMTS-13 has been reported in disseminated intravascular coagulation due sepsis. present study investigates whether...
Summary The plasma metalloprotease ADAMTS13 (A Disintegrin And Metalloprotease with ThromboSpondin type 1 motif 13) cleaves prothrombotic ultralarge multimers of the platelet‐adhesive protein von Willebrand factor (ULVWF) into less active that promote haemostasis in injured blood vessels. When enzyme is dysfunctional or undetectable, circulating ULVWF may cause massive intravascular aggregation platelets and thrombotic thrombocytopenic purpura. This study compared antigen activity a large...
Significance The blood protein von Willebrand factor (VWF) is required for platelets to stop bleeding at sites of injury, and the metalloprotease ADAMTS13 limits platelet adhesion by cleaving VWF only when flowing stretches it, especially within a growing thrombus. This feedback inhibition essential because deficiency causes fatal microvascular thrombosis. How recognizes so specifically not understood. We now find that folded roughly in half its distal domains inhibit domain. relieves this...
The majority of patients diagnosed with thrombotic thrombocytopenic purpura have autoantibodies directed towards the spacer domain ADAMTS13.In this study we explored epitope specificity and immunoglobulin class G subclass distribution anti-ADAMTS13 antibodies. anti-spacer antibodies was examined using plasma from 48 acute acquired by means immunoprecipitation ADAMTS13 variants containing single or multiple alanine substitutions. Using similar methods, also determined presence anti-TSP2-8...
We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost 100-fold differences when blind-testing 15 samples. Calibration relation to reference reagent and standard curve obtained by a dilution series reduced inter-laboratory variability ca 10-fold. The harmonisation quantification is vital step towards determining protective therapeutic levels antibodies.
Platelet-decorated von Willebrand factor (VWF) strings anchored to the endothelial surface are rapidly cleaved by ADAMTS13. Individual VWF string characteristics such as number, location, and auxiliary features of ADAMTS13 cleavage sites were explored here using imaging computing software. By following changes in length, we demonstrated that multiple times, successively shortening length function time generating fragments ranging size from 5 over 100 μm. These larger than generally observed...
Background Transfusion‐related acute lung injury ( TRALI ) is known as a life‐threatening complication of transfusion. HLA and HNA antibodies have been associated with the immune pathway . Since donors history transfusion and/or pregnancy are presumed to an increased risk carrying such antibodies, we investigated association or occurrence alloimmunization in our donor population. Study Design Methods A total 1018 female plateletpheresis male were enrolled study. Included systematically...
BACKGROUND Transfusion of cryopreserved platelets (cryoplatelets) is not common but may replace standard liquid‐preserved (PLTs) in specific circumstances. To better understand cryoplatelet function, frozen concentrates from different manufacturing sites were compared. STUDY DESIGN AND METHODS Cryoplatelets Denver, Colorado (DEN); Sydney, Australia (SYD); and Ghent, Belgium (GHE) compared (n = 6). A paired noncryopreserved control was included Ghent. Microfluidic‐flow chambers used to study...
Ultraviolet (UV) light illumination in the presence of exogenously added photosensitizers has been used to inactivate pathogens platelet (PLT) concentrates for some time. The THERAFLEX UV-C system, however, illuminates PLT with without additional photoactive compounds. In this study residual function is measured a comprehensive paired analysis UV-C-treated, gamma-irradiated, and untreated control concentrates.A pool-and-split design was buffy coat-derived 65% SSP+ additive solution. Thrombus...
BACKGROUND Pathogen inactivation and cold or cryopreservation of platelets (PLTs) both significantly affect PLT function. It is not known how PLTs function when are combined. STUDY DESIGN AND METHODS Standard concentrates (PCs) were compared to pathogen‐inactivated PCs treated with amotosalen photochemical treatment (AS‐PCT) stored at room (RT, 22°C), (4°C, n = 6), (−80°C, 8) temperatures. The impact alternative storage methods on arms was studied in flow cytometry, light transmittance...
BackgroundA disintegrin and metalloprotease 17 (ADAM17) catalyzes platelet glycoprotein (GP) Ibα ectodomain shedding, thereby releasing glycocalicin in plasma. The spatiotemporal control over the enzyme-substrate interaction biological consequences of GPIbα shedding are poorly understood.ObjectivesThis study aimed to determine by ADAM17.MethodsTransmission electron microscopy with immunogold staining, immunoprecipitation, quantitative western blotting were used.ResultsImmunogold staining...
Background Photochemical treatment ( PCT ) of platelet concentrates using photosensitizers and ultraviolet light illumination reduces the proliferation potential pathogens by damaging biomolecules. Materials Methods The impact riboflavin RF ‐ PRT )‐ amotosalen AS )‐based pathogen inactivation on platelets was studied microfluidic flow chambers immobilized collagen standard prepared from buffy coats in additive solution. Flow cytometry, metabolic parameters transmission aggregometry with...
Summary Background ‘Profound intellectual and multiple disability’ (PIMD) is defined as a profound cognitive disability with severe sensory motor impairments. The aim of this study was to evaluate the respiratory morbidity in children PIMD investigate possible risk factors. Methods In 10 specialized facilities for daily care patients PIMD, underwent standardized clinical assessement evaluating function. Additional medical information obtained. Results One hundred twenty seven aged 2–21 years...